Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,718,463	T→G	32.0%	Q65P (CAG→CCG)	CJ019_01614 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,718,463	0	T	G	32.0%	48.6 / 9.6	61	Q65P (CAG→CCG)	CJ019_01614	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (9/30);  new base G (8/13);  total (18/43)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTG  >  KT2440/1718333‑1718591                                                                                                                                   |                                                                                                                                 aTTGATTAACAGCAACTTTCCATTCAGCTCAAAGCGCGCACATACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGTAAGGCAGCGGCACGTTTCCATTGACCGGGCCGGCCAGgcg                                                                                                                              >  8:294034/1‑135 (MQ=255)   tgatgaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                             <  7:27285/134‑1 (MQ=255)   tgatgaACAGCAACTTCCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                             <  6:195669/134‑1 (MQ=255)   tgatcaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGGGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                             <  6:191826/134‑1 (MQ=255)       gaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTCCCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAg                                                                                                                        <  8:102188/135‑1 (MQ=255)         aCAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGACCGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAggg                                                                                                                      <  8:140864/135‑1 (MQ=255)             cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGGGCCAggggggc                                                                                                                  >  1:11232/1‑131 (MQ=255)             cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCACGGGCAc                                                                                                                  >  6:178408/1‑135 (MQ=255)             cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATAGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCAc                                                                                                                  >  2:14490/1‑135 (MQ=255)             cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCAc                                                                                                                  <  3:201391/135‑1 (MQ=255)             cAACTTGCCATTCAGATCAATGCGCGCACTTACAGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCTGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGGCGGGGTcca                                                                                                                  >  5:273228/1‑133 (MQ=255)                     cactccgCTCAATCCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGCCCGGCGCGGGGTCATAGGTGAGAGGGACCGGCAGCGGCACGTTGCCTTGGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAgggg                                                                                                          <  5:75158/129‑1 (MQ=255)                     cATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGGCATCGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAgggg                                                                                                          <  5:156454/135‑1 (MQ=255)                          aGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTc                                                                                                      <  4:47614/134‑1 (MQ=255)                                   cgcccACTTCCCGATTCGGGGGGGCCCGGCTTGCCGTGGGGGGGGCCATAGGGCAGATGGACGGGCTGGGGCACGTTGCCTTTGCCCGGGCCGGCCGGGCGCGCCTGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                            <  6:186860/135‑1 (MQ=255)                                    gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCCGGGGCGGCCCTCAGCGaca                                                                                           >  6:142734/1‑135 (MQ=255)                                    gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                            <  1:232176/134‑1 (MQ=255)                                              cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                             >  7:134738/1‑91 (MQ=255)                                              cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGgcgc                                                                                                                             <  8:134738/91‑1 (MQ=255)                                              cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCACGGCAGGGGCGTGCATCAGCGCCACAACCTGccc                                                                                 >  8:239167/1‑133 (MQ=255)                                              cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAg                                                                                 <  5:139768/135‑1 (MQ=255)                                              cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGGCACGGCCGGGGCGGGCCTCAGCGCCCCCGCCTGCAg                                                                                 >  3:117816/1‑135 (MQ=255)                                               gCATAGGGGTGGCCAGGCTTGACCGGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGt                                                                                <  6:59570/135‑1 (MQ=255)                                                    gggggggCCAGGCTTGCCGTGCGGGGGGTCATCGGTGCCAGGCACCGGCCGCGGCCGGTTGCGTTTCCCCGGGCCGGCGGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                           <  1:156103/130‑1 (MQ=255)                                                     ggggggCCTGGCTTGCCCTGCGCGGGGTCATTGGGGCGATGCAACGGCAGCGGCACGTTGCCTTTCACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                           <  7:294034/130‑1 (MQ=255)                                                     ggggggCCTGGCTGGAGGTGGGGGGGGGCATTGGGGAGAGGGACCGGCCGCGGCACGGGGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                           <  6:197649/130‑1 (MQ=255)                                                     cGGTGGCCAGGCTTGCCCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGGTGCGTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                           <  7:48374/134‑1 (MQ=255)                                                     cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGACCGGCAGCGGCACGTTGCCTTTGACCGGGCGGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                           <  6:123198/134‑1 (MQ=255)                                                     cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                           <  4:135686/134‑1 (MQ=255)                                                     cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACg                                                                          >  8:221841/1‑135 (MQ=255)                                                                 ttGACCGGCGCGGGGTCATCGGGCCGAGGCACCGCCCGCGCCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                              <  5:201221/135‑1 (MQ=255)                                                                  tGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGGCCCAGGGGAACGGCCGGGGCGTCCATGCGCGCCACCACCGTGAGTTACACGCGGAGGGTggggg                                                             >  2:257773/1‑135 (MQ=255)                                                                      cTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                         <  1:177790/135‑1 (MQ=255)                                                                      cTGCGCGGGGGCATAGGTGCGATGGAACGGCAGCGGCACGGTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                         <  2:40729/135‑1 (MQ=255)                                                                       tGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCCCGGCAGGGGCGGCCATCAGCGCCACATCCGGCAGGTCCACGCGCAGGGTggcggccggc                                                        >  7:66167/1‑135 (MQ=255)                                                                           cgGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcaggcggca                                                    <  8:53951/135‑1 (MQ=255)                                                                            ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGGGCCTCAGCGCCCCAACCTGCAGGTCCACGCGCAGGGTggcggccggcggccc                                                   >  4:37767/1‑133 (MQ=255)                                                                                  atcggTGAGCTGCAACGGCAGCGCCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAAt                                             <  3:269279/132‑1 (MQ=255)                                                                                      gTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                         <  4:83333/135‑1 (MQ=255)                                                                                       tGAGATGGAACGGCTGCGGCACTTTGCCTTTGACCTGGCCTGCCTGGCTCGCCAGGTTCACTTCAGGGGAGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGTAAATgctc                                         <  6:273228/134‑3 (MQ=255)                                                                                       tGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                         <  6:97826/134‑1 (MQ=255)                                                                                         agaTGCAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGc                                      <  6:291283/135‑1 (MQ=255)                                                                                           agggcccGGCCGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAg                                    <  1:263689/129‑1 (MQ=255)                                                                                            tGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGGCCATCCGCGCCACATACTGCAGTTGCCCGCTCAGGGTGGGGGCAGGCGGCAGGGCAATGCGCTGCAgg                                   >  8:136634/1‑135 (MQ=255)                                                                                             gtaaCGGCTGCGGCACGTGGCCTTTGACCGGGCGGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGGTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGt                                  <  4:278846/133‑1 (MQ=255)                                                                                             ggAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCGGGGGGCGCGACCGTCCCGCGAGGGGCGGCCATGCCAGCCACAAACGCCCTTTCCCCGCTCACCGCGGCGGCAGGCCGCGGCGCAAGTCGCTGGAGGt                                  >  4:121214/1‑135 (MQ=255)                                                                                             ggAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCACGGCAGGGGCGGCCCTCAGCGCCCCCTCCTGCAGTTCCACGCGCAGGGTGGCGGCCGGCGGCAGGGCAATGCGCTGCAGGt                                  >  8:123762/1‑135 (MQ=255)                                                                                             ggAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGCCACATCCTGCAGTTCCCCGCGCAGGGTGGCGGGAGGCGGGAGGGCAATGCGCTGCAg                                    >  6:87852/1‑133 (MQ=255)                                                                                              gAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCCCGGTCACCGCCGGGGCGGCCAGCAGCCACACATCCTGCCGTTACACCCCCATGGGGGCGGCCGGCGGCAGGGCCATTCGCTGCAg                                    >  6:208881/1‑132 (MQ=255)                                                                                                cctgctgCGGCACGTGGCCTTTGCCCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTaga                               <  2:267871/129‑1 (MQ=255)                                                                                                     aGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                          <  4:132917/135‑1 (MQ=255)                                                                                                         gCAGGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCGGCAGGTAGAAGACTTCAc                      <  7:221764/135‑1 (MQ=255)                                                                                                         gCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCAc                      <  7:136634/135‑1 (MQ=255)                                                                                                              ttGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTcc                 <  5:292263/135‑1 (MQ=255)                                                                                                                      gACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTgg          <  7:248021/134‑1 (MQ=255)                                                                                                                      gACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTgg          <  7:243938/134‑1 (MQ=255)                                                                                                                       aCCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGcc        <  4:71296/135‑1 (MQ=255)                                                                                                                            gccggccTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGtt   <  5:208881/135‑1 (MQ=255)                                                                                                                             ccggctTGGGGCGCGAGGGGCACGGCAGGGGCGTCCATCAGCGACTCATCCTGCAGTTCCACGCTCAGGGTGGCGGAAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTtg  <  8:114168/135‑1 (MQ=255)                                                                                                                             ccggccTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTtg  <  4:174085/135‑1 (MQ=255)                                                                                                                             ccggccTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTtg  <  7:239167/135‑1 (MQ=255)                                                                                                                                   |                                                                                                                                 ATTGATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTG  >  KT2440/1718333‑1718591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑