Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 96,466 T→G 27.5% intergenic (+107/‑86) cynT → / → CJ019_00104 Carbonic anhydrase 1/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT244096,4660TG27.5% 49.2 / 2.3 40intergenic (+107/‑86)cynT/CJ019_00104Carbonic anhydrase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/10);  new base G (3/8);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 7.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCACTCCGAGAGGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGT  >  KT2440/96336‑96600
                                                                                                                                  |                                                                                                                                      
gcCACTCCGAGAGGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTggcg                                                                                                                                    <  4:56534/135‑1 (MQ=255)
       cGAGAGGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCcgcg                                                                                                                             >  4:80556/1‑135 (MQ=255)
            ggCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGgc                                                                                                                        >  8:253345/1‑135 (MQ=255)
            ggCAGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgc                                                                                                                        <  2:261159/135‑1 (MQ=255)
               cGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTGGGGGGCCGTGAATTCCCTGCCAGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCCgggcg                                                                                                                     >  2:251568/1‑135 (MQ=255)
                 ctactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGGCGTCGGCTGCCGTGAATTCCCAGACAGCCCAGCCCGCCTGGACGCTGGCGGCCCGCCCCGgggcgcg                                                                                                                   >  3:86686/1‑134 (MQ=255)
                  tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCtt                                                                                                                  <  3:92885/135‑1 (MQ=255)
                  tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTGGGGGGGCGTGTATTAGCTGGCGGGCCAGGCGGCGGGGTGGCCGGCGGGCCGCGCCGgggcgcgg                                                                                                                  >  5:213918/1‑133 (MQ=255)
                    ctaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCGTg                                                                                                                 >  8:251709/1‑134 (MQ=255)
                          gACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCTTGTCAgggg                                                                                                          >  7:88215/1‑135 (MQ=255)
                          gACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCGGCCTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGAGGCGGCGCGCGTGGCCgggg                                                                                                          >  8:18651/1‑135 (MQ=255)
                            cccGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTTGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGc                                                                                                         >  7:75773/1‑134 (MQ=255)
                            cccGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCa                                                                                                        <  4:167113/135‑1 (MQ=255)
                              cGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGCATTCCCTGTCTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCCCCTGCGCGCGTGTCCGGGGCaac                                                                                                      >  8:104193/1‑134 (MQ=255)
                               gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTAGCTGGCGGCCCGCGCCGGCGCGCGTGTCAGGGGCAAAc                                                                                                     >  4:243569/1‑135 (MQ=255)
                                 ccgccgCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGGGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGt                                                                                                   <  6:239994/135‑1 (MQ=255)
                                         acaaCCTTGATCGCCGGCGGCACCCTTCAGGGGTGGGGGGCGGCCTTCGGCTGCCTTGAATGCCCTGCCTGCCCGGCGGGCGGGCGCGCGGGCGGCCCGCGCCGGCGGGCTTGTCCGGGGCAAACGTGCCCACg                                                                                            <  8:259814/132‑1 (MQ=255)
                                                    aGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGAGCCCCAGGCCCGGGGGATg                                                                                >  8:89274/1‑135 (MQ=255)
                                                    aGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTACCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCGTGTCAGGGGGAAAAGTGCCCACGGCCAGGGGAATg                                                                                >  3:104753/1‑135 (MQ=255)
                                                    aGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTACCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCCCGTGTCAGGGGCAAACGTGCCCACGGCCAGGGGaaag                                                                                >  8:93317/1‑133 (MQ=255)
                                                     gCCGGCTGCACCCTTGCGGGGGGGGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCGGCGCGCTTGTCCGGGGCAAACGTCCCCACGGCCAGGGGAATgg                                                                               <  1:251568/135‑1 (MQ=255)
                                                            gCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCg                                                                        >  7:231963/1‑135 (MQ=255)
                                                                     aGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCGGTCTGCCCTGCCGGCGTGCGAGCTGGCGGCCCGCGCCGGCGCGCGTGGGCGGGGCCAACGTGCCCACCGCCCGGGGGATGGCCGGGCGGCGAGAGaa                                                               >  4:115576/1‑135 (MQ=255)
                                                                              gcggGGCTTGCGGCTGCCTTTAATTCCCTGCCTGCCCTGCCGGGGTGCGCGCGGGGGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAAGGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAac                                                      <  2:269226/135‑1 (MQ=255)
                                                                                ggggCGTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCGGGCGTGCTCGCTGGCGGCCCGCGCGTGCGCGCTTGTCAGGGGAAAACATGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                    <  7:143005/133‑1 (MQ=255)
                                                                                gcgGCCTTGGGCTGCCTTTAATTCCCTGACTGCCCGGCCGGCGTGCGCGGGGGCGGCCCGCGCCTGGGCGGTTGTCCGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                    <  5:291498/135‑1 (MQ=255)
                                                                                gcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                    <  8:231963/135‑1 (MQ=255)
                                                                                gcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                    <  6:50571/135‑1 (MQ=255)
                                                                                gcgGCCTGCGGGTGCCTTTAATCCCCTGACGGCCCTGCGGGCGTGCTCGCGGGGGGCCCGCGCCGGCGCGCTTGTCTGGGGCAAACGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                    <  3:110599/135‑1 (MQ=255)
                                                                                gcgGCAGTCGGTGGCCTTTAATTCCCTGCCTGCCCTGCGGGGGTGGTGGCGGGCGGCCGGCGCGTGGGCGTTTGTCAGGGTAAAACGGCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                    <  2:235108/135‑1 (MQ=255)
                                                                                      ttCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCCCCCCTGCGCGCGTGGAAGGGGCAAACCTGCCCACCGCCCCGGGGATTGCCGTGCGACGAGAGAAAAGAGAAACACAGTGa                                               >  4:259081/1‑134 (MQ=255)
                                                                                      gtCGGGTGCCTTGAATTACCTGACTGCCCTGCCCGCGTGGGCGCGGGCCGCCCGCGGCAGCGCGCTTGTCAGGGGCAAAACAGACCACGGCCAAGGGTAATGCCGGTCGCCCTGAGAAAAGACAAACACCGTGaa                                              >  8:206955/2‑135 (MQ=255)
                                                                                          gctgctTTTAATTCCCTGACTGCCCTGCCGGCGTGCGCGGTGGCGGCCGGCGCCGGCGGTGTTGTCAGGGTAAAACGTCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt                                          <  1:7590/135‑1 (MQ=255)
                                                                                                 tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                    <  7:198496/118‑1 (MQ=255)
                                                                                                 tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                    >  8:198496/1‑118 (MQ=255)
                                                                                                 tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGt                                   >  2:192977/1‑135 (MQ=255)
                                                                                                        ccTGACGGCCCTGCCGGCGTGCTCGGGGGCGGCCCGCGCCGGGGCGCTTGTCCGGGGCAAACGGCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGc                            <  8:75773/135‑1 (MQ=255)
                                                                                                                ccctgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGGATGGCCGTGCGACGAGAGCAAGGAGAAACACGGTGCACATTACACAGTTGAAAGCGGCCCTGc                    >  2:162888/1‑135 (MQ=255)
                                                                                                                ccctgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACCGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGcccggg                    >  7:149655/1‑132 (MQ=255)
                                                                                                                               cGCTGGCGGCCGGCGCCTGCGGGGTTGGCTGGGGAAAACGTGCCCACGGCCAGGGGAATGGCGGTGCTAATAGAGAACGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTgg     <  4:73503/135‑1 (MQ=255)
                                                                                                                                  tGGCGGCCCGCGCCTGCGCGCTTGGCAGGGGCAAACGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGt  <  2:31109/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                      
GCCACTCCGAGAGGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGT  >  KT2440/96336‑96600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑