Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,791,678 T→G 34.3% L182R (CTG→CGG)  lpxB → Lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,791,6780TG34.3% 22.3 / 3.0 30L182R (CTG→CGG) lpxBLipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  ref base T (15/2);  new base G (4/6);  total (22/8)
Fisher's exact test for biased strand distribution p-value = 2.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGC  >  KT2440/1791558‑1791800
                                                                                                                        |                                                                                                                          
cGCTGCGGCCGGGCGCGGCGCGGTTCTACCAAGCGCAGGGGGGGCCAGGGCGTTTTGGCGGCCACCCGCGGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGAgg                                                                                                              <  4:102302/135‑1 (MQ=255)
  ctgctgCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGGCGCGCTGGGCCTGGGCGAgggg                                                                                                            >  2:244405/1‑135 (MQ=255)
      tgcCGTTCGCGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGGCCCCCGCGGGCAGCCCCCCTACCGCGTGCCGCCGACCGCCCCGCCGCGGCTGCCGCGCGGGGGCGGGGGGCGGGGCCgg                                                                                                        >  2:133768/1‑135 (MQ=255)
                  cgcgGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGGGGTGGCGTTGa                                                                                            >  6:129880/1‑135 (MQ=255)
                        tCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCCCAGGGCCTGGGGCCGGGGCCCGGGGTGGGGGTGATGcccg                                                                                      >  3:227595/1‑133 (MQ=255)
                                 cgCAGGGGGGGCCAGGGCGTTTTGGCGGCCACCCGCTGGCAGACACCATCCCGCTTGACGCCGCCCGCCCGGCCGCGCGTGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCggg                                                                             <  6:70585/134‑1 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                          >  6:281296/1‑135 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGGGGTGGCGGTGATGCCGGGCCGCCgggggg                                                                          >  5:70585/1‑133 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCCGCCGGGGTg                                                                          >  3:116578/1‑135 (MQ=255)
                                    aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                          >  6:126837/1‑135 (MQ=255)
                                              aGTGCGTTTTGTCGGCCACCCGCTGGCAGACCCCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGCCGCGCCGGGCCTGGGGGCGGGGCCGGTGGTGGCGGTGCTGCCGGGCCGCCGGGGTGGCGTaggagg                                                                >  4:6884/1‑135 (MQ=255)
                                              aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGGTGCTGGCGGGCCGCCGGGGTGGCGAAGTAgg                                                                >  3:21911/1‑135 (MQ=255)
                                                   gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCt                                                           >  4:34447/1‑135 (MQ=255)
                                                     tttGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCAGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCCGCCGGGGTGGCGCAGTAGGGCCCTTg                                                         >  4:78395/1‑135 (MQ=255)
                                                         tCGCCCACCCGCTGGCAGACACCATACCGCTTGACCCCGACGGCCCGGCCGCGCGTGCGGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                      <  4:227595/134‑1 (MQ=255)
                                                                        cAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGa                                        <  4:146781/133‑1 (MQ=255)
                                                                              ccATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCAGGGCGAGGGGCCGGTGGTGGGGGTGGTGGCGGGCCGCCGGGGGGGCGCAGTAGGGCGCTTGGGCGCGCGGTTGCGCGCCGcccccg                                >  5:254363/1‑135 (MQ=255)
                                                                                aTACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAg                              >  2:86335/1‑135 (MQ=255)
                                                                                    cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGcc                          >  6:128476/1‑135 (MQ=255)
                                                                                         gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGCCGCCGCCGAGCGCCTCAGc                     >  1:252875/1‑135 (MQ=255)
                                                                                         gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                     >  4:218918/1‑135 (MQ=255)
                                                                                         gAAGCCGACCGCCCGGCCGCGCCTGCCCCCCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTTATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCGAGACGCCGCCGAGCGCCTCAGc                     >  5:163000/1‑135 (MQ=255)
                                                                                            gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCCGccca                  >  7:273553/1‑133 (MQ=255)
                                                                                                   gCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGGTGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCGTGGGCGCGCGGGTGCTCGCCGCCGCCGAGCGCCTCCGCCACCCAGTGc           >  6:4183/1‑135 (MQ=255)
                                                                                                   gCCCGGCCGCGCGTGCCGCGCGGGGCCGGGGCGCGGGGCCGGTGGTGGCGGTGGTGCCGGGCCGCCGGGGGGGCGGAGTAGGGCGCGTGGGCGCGCGGTTCCGCGGCGCCGCCGCCCGCCGCCGCCCACAACTGc           >  4:191878/1‑135 (MQ=255)
                                                                                                            cgcgTGCCGGGGGGGGCGGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGTGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCTGGGTGTgc  <  1:156966/135‑1 (MQ=255)
                                                                                                            cgcgTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGCAGTAGGGCGCGTGGGCGCGCGGTTCCTCGACGCCGCCGAGCGCCGCAGCCAACAAGTGCCGGGTGTgc  >  1:253203/1‑135 (MQ=255)
                                                                                                            cgcgTGCCGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc  <  7:6405/135‑1 (MQ=255)
                                                                                                            cgcgGGCGGCGCGGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc  <  7:215313/135‑1 (MQ=255)
                                                                                                            cgcgGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc  <  5:129880/135‑1 (MQ=255)
                                                                                                                        |                                                                                                                          
CGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGC  >  KT2440/1791558‑1791800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑