Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,830,632	G→A	7.3%	P65P (CCG→CCA)	CJ019_01746 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,830,632	0	G	A	7.3%	164.8 / 3.0	55	P65P (CCG→CCA)	CJ019_01746	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base A (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

AGGCTTTCCTGCCGCAGCCGCGCACGCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTGGGCAAGCTCAAGAGCG  >  KT2440/1830502‑1830753                                                                                                                                   |                                                                                                                          aGGCTTTCCTGCCGCAGCCGCGCACGCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGc                                                                                                                         <  7:209042/133‑1 (MQ=255)   gCTTTCCTGCCGCAGCCGCGCACGCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTc                                                                                                                     <  4:138992/135‑1 (MQ=255)    cTTTCCTGCCGCAGCCGCGCACGCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCg                                                                                                                    <  1:75951/135‑1 (MQ=255)      ttCCTGCCGCAGCCGCGCACGCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGa                                                                                                                   >  6:240616/1‑134 (MQ=255)           gCCGCAGCCGCGCACGCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGgt                                                                                                             >  8:117892/1‑135 (MQ=255)              gCAGCCGCGCACGCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGtt                                                                                                          <  2:211929/135‑1 (MQ=255)                     cgcACGCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGc                                                                                                   <  5:152045/135‑1 (MQ=255)                          gCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGa                                                                                              <  5:79657/135‑1 (MQ=255)                             ggCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGAccc                                                                                           <  2:274519/135‑1 (MQ=255)                             ggCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGAccc                                                                                           <  2:278089/135‑1 (MQ=255)                                  aaCTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGgag                                                                                       >  4:290678/1‑134 (MQ=255)                                  aaCTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGgag                                                                                       >  7:39410/1‑134 (MQ=255)                                       tgcgACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAgtg                                                                                 <  1:237999/134‑1 (MQ=255)                                            aaTCAGTGACGACCGCTACCTGTCGACATTGTCCCTGCGCGTGTTCAGCGCAGCGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCTTTCTCGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGc                                                                             >  8:6286/2‑134 (MQ=255)                                                    acgacCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGgcg                                                                    <  8:235980/135‑1 (MQ=255)                                                     cgacCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGgcgc                                                                   <  8:280518/135‑1 (MQ=255)                                                     cgacCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGgcg                                                                    <  4:149967/134‑1 (MQ=255)                                                          gCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGc                                                              >  8:176844/1‑135 (MQ=255)                                                          gCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGc                                                              >  8:258362/1‑135 (MQ=255)                                                              ccTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCt                                                          >  2:49475/1‑135 (MQ=255)                                                               cTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCt                                                          <  2:19091/134‑1 (MQ=255)                                                                  tCGACATTGGCCCTGCGCGTGGTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAg                                                      <  1:146159/135‑1 (MQ=255)                                                                     aCATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCg                                                    <  2:28248/134‑1 (MQ=255)                                                                      cATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGt                                                  >  7:153276/1‑135 (MQ=255)                                                                        ttGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTg                                                <  5:110149/135‑1 (MQ=255)                                                                          ggCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTg                                                <  4:77533/133‑1 (MQ=255)                                                                              cTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAg                                          <  8:224067/135‑1 (MQ=255)                                                                               tGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAgg                                         >  3:135923/1‑135 (MQ=255)                                                                                   cgTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGa                                     >  6:266169/1‑135 (MQ=255)                                                                                   cgTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGa                                     >  5:224569/1‑135 (MQ=255)                                                                                   cgTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGAGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGAGCCGAGCGTCTGGAGCGGTTGATGCAGGATGa                                     >  7:257388/1‑135 (MQ=255)                                                                                    gtgtTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAg                                    >  7:52630/1‑135 (MQ=255)                                                                                        tCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCa                                >  8:6184/1‑135 (MQ=255)                                                                                         ccGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCa                                >  6:132736/1‑134 (MQ=255)                                                                                              cgGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATcc                          >  5:199381/1‑135 (MQ=255)                                                                                              cgGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATcc                          >  6:296160/1‑135 (MQ=255)                                                                                              cgGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATcc                          >  5:14578/1‑135 (MQ=255)                                                                                                      aaGCACAGCCTGGTGGCTGCCTAGTGGCCGGCGTTAGAGCAGGTGTTCTTTGGCTTCGAGCCGGAGACTGTGGTGCTGATGGGGGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGACACCTg                  <  6:137271/135‑1 (MQ=255)                                                                                                       aGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  <  2:58229/134‑1 (MQ=255)                                                                                                       aGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  <  6:224569/134‑1 (MQ=255)                                                                                                       aGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  <  6:59262/134‑1 (MQ=255)                                                                                                       aGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  <  5:146291/134‑1 (MQ=255)                                                                                                       aGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  <  5:150974/134‑1 (MQ=255)                                                                                                       aGCACAGCCTGGGGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  <  7:39596/134‑1 (MQ=255)                                                                                                       aGCACAGCCTGCTGGATGCCAAGTGGCCGGCGTTCGAGCAGGGGGTGTTTTGATTCGACCGGGAGAAAGTGTTGCTGATGGGCGCCGAGCAGCTGTAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  <  6:245615/134‑1 (MQ=255)                                                                                                         cacaGCCTGGTGGATGGCTAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTGGGc               <  1:220716/135‑1 (MQ=255)                                                                                                              ccTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTGGGCAAGc           >  7:88326/1‑134 (MQ=255)                                                                                                              ccTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTGCGCAAGc           >  3:105656/1‑134 (MQ=255)                                                                                                              ccTGGTGGATGCCAAGTGGCCAGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  <  5:187064/127‑1 (MQ=255)                                                                                                              ccTGGTGGATGCCAAGTGGCCAGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  <  5:190807/127‑1 (MQ=255)                                                                                                              ccTGGTGGATGCCAAGTGGCCAGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  >  6:190807/1‑127 (MQ=255)                                                                                                              ccTGGTGGATGCCAAGTGGCCAGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTg                  >  6:187064/1‑127 (MQ=255)                                                                                                                tggtggATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTGGGCCAGCt          >  6:221034/1‑133 (MQ=255)                                                                                                                      aTGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTGGGCACGCTAAAGAGCg  >  2:222586/1‑135 (MQ=255)                                                                                                                      aTGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTGGGCAAGCTCAAGAGCg  >  3:15688/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                          AGGCTTTCCTGCCGCAGCCGCGCACGCCGGCGCAACTGCGCGACATCAGTGACGACCGCTACCTGTCGACATTGGCCCTGCGCGTGTTCCGCGCGGGGCTCAAGCACAGCCTGGTGGATGCCAAGTGGCCGGCGTTCGAGCAGGTGTTCTTTGGCTTCGACCCGGAGAAAGTGGTGCTGATGGGCGCCGAGCATCTGGAGCGGTTGATGCAGGATGAGCGCATTATCCGCCACCTGGGCAAGCTCAAGAGCG  >  KT2440/1830502‑1830753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑