Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,834,908	T→G	37.1%	T38P (ACC→CCC)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,834,908	0	T	G	37.1%	37.0 / 4.9	35	T38P (ACC→CCC)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (14/7);  new base G (6/8);  total (20/15)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAA  >  KT2440/1834775‑1835037                                                                                                                                      |                                                                                                                                  aCGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGgtg                                                                                                                                  <  2:260005/135‑1 (MQ=255)               ggccgAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTTCGTCATTGATTAACGCGCAGACCACCTCAAAGCCATCTGCCAGGGAGGGGGTGGCGACGGTGAggcg                                                                                                                    >  8:100047/1‑135 (MQ=255)                   gAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGGGTTCGTGATTGTTGAAGGCGCAGACCCCCAGCAACCCCAAGGCCCGGGCGGCGGGGTGGAGGGGGAggcgggcg                                                                                                                >  5:244515/1‑135 (MQ=255)                    aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTGGCGTTCGGCATTGATGAATGCGCAGCCCCCCGCAACGACCTCGGCCCGGGCGGCGGGGTCGGGGGTCAGgcgggcgg                                                                                                               >  2:161262/1‑135 (MQ=255)                    aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGGCGAGGGTCAGgcgggcgg                                                                                                               >  8:292067/1‑135 (MQ=255)                    aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGGGCGTTGGGGATTGATGAAGGCGCCGCCCCCCGCAAAGCCCTCTGCCAGGGCGGCGGGGTGGGGGGGCAGgcgggcgg                                                                                                               >  1:228461/1‑135 (MQ=255)                       gcAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTGGTGATTGATGAAGGCGCCGACCACCGCAAAGCCATCTGGCAGGGGGGCGGTGGCGTGGGTGAGGCgggcgggcg                                                                                                            >  6:66200/1‑134 (MQ=255)                            gCGATCACCCGCGGGCCAGCGGCGGCCCGGCGCGGCAGCACCGGCGCGTCGAGTGCGGCATTGAGGAAGGCGCAGCCCACCTCAACCCCAGCGGCCAGGGCGGCGGTGTCGCGGGGCAGGCGGGCGGGCTGGaaa                                                                                                       <  7:145178/135‑1 (MQ=255)                            gCGATCAACCGCGGGCCCGGGGCGGCCAGGCGCTGCAGCACCGGCGGGGCTAGTTCGTCATTGATGACGGCGCACACCACCCCAAACCCATCTCCCCGGGCGGGGGTGTCGAGGGCCAGGCGGGCGGGCTGGaaa                                                                                                       <  2:67555/135‑1 (MQ=255)                                  aaCCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTGGGGGTCGTCCTTGATGAAGGCGCAGACCCCCGCCAAGCCCTCTGCCAGGGCGGGGGGGTCGGGGGTCAGGCGGGGGGGCGGGGAATGCAGc                                                                                                 >  2:210996/1‑135 (MQ=255)                                  aaCCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGCGGGTCCGGCGGGCGGGCTGGAAATGCAGc                                                                                                 >  4:42623/1‑135 (MQ=255)                                   aCCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGCCCCCCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCCGcg                                                                                                >  7:45029/1‑134 (MQ=255)                                      gcgGGCCAGCGGCGGCCTGGGGCTGCAGCACCGGCGCGTCGAGTGCGTCATTGTTGACGGCGCAGCCCACCACAAACCCATCTGCCCGGGCGGCGGGGTCGCGGGTCCGGGGGGGGGGCTGGAAATGCAGCGCCa                                                                                             <  6:70093/135‑1 (MQ=255)                                               cggcggCCAGGCGCTGCAGCACCGGCGCGTGGAGTTGGTCATTGATGAAGGCGCCGACCACCTCAAAGCCCTCTGCCCGGGGGGGGGGGTCGGGGGTCGGGCGGGCGGGGGGGGAATGGCGCGGCCGGGCGGTgc                                                                                    >  4:284028/1‑135 (MQ=255)                                                                agcaCCGGCGCGTCGAGTGCGTCATTGATCACGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGGGGTGGCGAGGGGCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTg                                                                   <  2:204663/135‑1 (MQ=255)                                                                          cgTCGAGTTCGTCATTGATGAAGGCGCAGCCCACCTCAAAGCCATCTGCCAGGGCGGCGGGGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCTGGCAGCCTTGGTGAAGCtttgc                                                          >  3:298874/1‑132 (MQ=255)                                                                                    gTCATTCATGACGGCCCACACCCCCTCAACCCCAGCTGCCTGGGCGGGGGGGTCGAGGGGCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGt                                                <  3:190709/134‑1 (MQ=255)                                                                                        ttGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGTGGGTCTGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgctgg                                           <  3:42623/135‑1 (MQ=255)                                                                                                    cAGACCACCGCAACGCCAGCGGCCTGGGGGGGGGGGTCGCGGGGCTGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGaacaac                               <  4:17269/135‑1 (MQ=255)                                                                                                     aGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAacaaca                              >  4:35209/1‑135 (MQ=255)                                                                                                         caccTCAACGCCACGTGCCTGGGCGGCGGTGGCGCGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgc                          <  7:292067/135‑1 (MQ=255)                                                                                                         caccTCAAAGCCATCTGCCTGGGCGGCGGGGTCGTGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgc                          <  3:129511/135‑1 (MQ=255)                                                                                                           ccTCAAAGCCAGCTCCCTGGGGGGGGGGGTCGTGGGTCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgcgc                        <  2:66438/135‑1 (MQ=255)                                                                                                               aaaGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCAt                    >  4:241705/1‑135 (MQ=255)                                                                                                               aaaGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCGGGGTGAAGCGTTCCTGATCGGAGTGCGGGCGGCGGAACAACAGGGCGCGCag                    >  5:26802/1‑134 (MQ=255)                                                                                                                 cgCCAGCTCCCAGGGCGGCGGGGTCGAGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                  <  2:228461/134‑1 (MQ=255)                                                                                                                   ccAGCTGCCCGGGCGGCGGGGTCGCGGGGCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTc                <  3:200237/135‑1 (MQ=255)                                                                                                                      gcggCCTGGGCGGCGGGGGCGTGGGTCAGGCGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGaa             <  1:68795/132‑1 (MQ=255)                                                                                                                         gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACACCCGGGCGCGCATGGTCGACCTc          >  2:276437/1‑135 (MQ=255)                                                                                                                         gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAtctc          >  6:126614/1‑135 (MQ=255)                                                                                                                         gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc          >  5:107862/1‑135 (MQ=255)                                                                                                                         gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc          >  6:36322/1‑135 (MQ=255)                                                                                                                         gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc          >  5:102702/1‑135 (MQ=255)                                                                                                                         gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGAGCGCATGGTCGAACTc          >  6:286365/1‑135 (MQ=255)                                                                                                                                 gggggTGTCGTGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCaa  <  4:136642/132‑1 (MQ=255)                                                                                                                                 ggcggTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCaa  <  2:241008/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  ACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAA  >  KT2440/1834775‑1835037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑