| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,839,275 | G→T | 12.9% | L170L (CTG→CTT) | pfeS_2 → | Sensor protein PfeS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,839,275 | 0 | G | T | 12.9% | 77.0 / 4.9 | 31 | L170L (CTG→CTT) | pfeS_2 | Sensor protein PfeS |
| Reads supporting (aligned to +/- strand): ref base G (17/10); new base T (2/2); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
ATACCGTTCCCGGGGGCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTGTCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCG > KT2440/1839144‑1839408 | aTACCGTTCCCGGGGGCGCCGGAGCAGGGCATGCAGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCtgttg > 5:101122/1‑135 (MQ=255) tagcggtCCCGGGGGCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGAACCGCTTGCTGTGGCTCATTGTCACCAACGGCATCATCCCGGGTCTGTTCACCTTGCtgttgt < 2:279526/129‑1 (MQ=255) tACCGTTCCCGGGGGCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCtgttgt < 1:161589/135‑1 (MQ=255) tACCGTTCCCGGGGGCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCtgttgt < 5:147062/135‑1 (MQ=255) cGTTCCCGGGGTCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTGAATGCGGGCCAGCACCGGTTGCTGTGGCGCATTGTCACCAACGGCATCATCCAGGGGCTGTTCACCTTGCTGTtgtgt < 6:237312/134‑1 (MQ=255) cGTTCCCGGGGGCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTtgtgt < 3:176719/134‑1 (MQ=255) cGTTCCCGGGGGCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTtgtgt < 4:212534/134‑1 (MQ=255) gggggCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTTTTGTGTGTa < 8:77670/130‑1 (MQ=255) gggggCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTTTTGTGTGTa < 8:73780/130‑1 (MQ=255) gggggCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTTTTGTGTGTa > 7:77670/1‑130 (MQ=255) gggggCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTTTTGTGTGTa > 7:73780/1‑130 (MQ=255) gggggCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCt > 5:120082/1‑135 (MQ=255) gggCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGt < 7:301076/135‑1 (MQ=255) gcCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTAcc > 8:117059/1‑134 (MQ=255) cGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCa > 7:139737/1‑135 (MQ=255) aTGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTg < 6:120082/135‑1 (MQ=255) tGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCCTCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCt < 5:301425/133‑1 (MQ=255) tGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCt < 7:272873/133‑1 (MQ=255) tGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCt < 7:90656/133‑1 (MQ=255) aTCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAAc > 4:33841/1‑135 (MQ=255) gAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCaa > 5:280542/1‑135 (MQ=255) tGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTc > 8:104698/1‑135 (MQ=255) cGCAGCGCTTCAATCCGGGCCAGCACCGCTTTCTGTGGCGCATTGTCACCCACGGCATCATCCCGGGGCTGTTCCCCTTGCTGGTGTGTGTAGGCCTGTACCGCATGCTGCTCGTGCCGCTGCACCACCTTcg > 5:277233/1‑133 (MQ=255) ttCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGCTCGTGCCGCTGAACCAACTTCGCGAGCAGGc > 8:157577/1‑134 (MQ=255) aGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCggg > 2:119766/1‑135 (MQ=255) gCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCCCCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCCGCt > 4:146658/1‑135 (MQ=255) gCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTa > 5:96248/1‑61 (MQ=255) gCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTa < 6:96248/61‑1 (MQ=255) cACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCCCTTCGCTGTGGGGCCGCCgggg > 6:55160/1‑134 (MQ=255) cAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGACCCAACTTCGCGAGCAGGCCAATGCCTGGCGGGGCGCGGAGCGGGCGGGCCGCCgggccgg > 1:144591/1‑131 (MQ=255) cTGTTCACATTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCAAATGCCTAGCGGGCCGAACAGCTGTAGGCCCGCCTGGACTAGCCCACCAACGCCCGGCa > 6:218944/1‑134 (MQ=255) aCCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCTTGCCGCTGAACCAAATTCGCGAGCAGGCCAATGCCTGGCGGGCCGCTCAGCAGTCCGCCCGCCTGGGCACGGGCACCAACCCCCGGGAAGACCaa > 6:43763/1‑135 (MQ=255) aCCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTcgc < 6:248923/60‑1 (MQ=255) aCCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTcgc > 5:248923/1‑60 (MQ=255) aCCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTGTCGGCCCGCCGGGACTCGCGCCCCATAGCCCGGCCTGACGaa > 8:30516/1‑135 (MQ=255) ggtggatgttggGTGTGGGCCGTTCCCCCATCCTCCTCGTGCCGCGAACCCAAGTGCGCGAGCCGCCAATTGCCTGGGGGGCCGATCAGCTGGGGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAAt < 8:235249/129‑1 (MQ=255) tgttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGCTCAGCTGTCGGCCCGCCGGGCCTCGCGCCCCAAACCCCCGCAAGACGAATTTGGCg > 1:76171/1‑135 (MQ=255) | ATACCGTTCCCGGGGGCGCCGGAGCAGGGCATGCTGGTGATCGAACTGCCGCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTGTCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCG > KT2440/1839144‑1839408 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |