Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,076,973 G→A 8.7% L190L (CTG→CTA)  CJ019_01978 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,076,9730GA 8.7% 139.1 / 4.1 46L190L (CTG→CTA) CJ019_01978hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (18/24);  new base A (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

GCGGCTTGTTCGGGGTGGGTGGGGCCGTGGTAGCCACGCCGATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTC  >  KT2440/2076846‑2077105
                                                                                                                               |                                                                                                                                    
gcggcTTGTTCGGGGGGGGTGGGGCCGTGGTAGCCACGCCGATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCt                                                                                                                               <  2:30775/135‑1 (MQ=255)
      tGTTCGGGGTGGGTGGGGCCGTGGTAGCCCAGCCGATCCTGACCAGTGTGTTTGGCGCGACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGAGCCGAGTCCCTTGGTAACCCTGCTGACCTATGGCg                                                                                                                         >  6:276878/1‑135 (MQ=255)
           ggggTGGGGGGGGCCGTGGTAGCCACGCCGATGCTGACCAGTGTGTTTGGCGCTAGTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGca                                                                                                                     <  4:208287/134‑1 (MQ=255)
               tgggggggGCCGTGGTAGCCACGCCGATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACcaca                                                                                                                <  1:179958/135‑1 (MQ=255)
               gggggggggCCGTGGTAGCCACGCCGATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACcaca                                                                                                                <  3:2426/134‑1 (MQ=255)
                    ggggCCGTGGTAGCCACGCCGATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGtt                                                                                                           >  2:66579/1‑135 (MQ=255)
                            ggTAGCCACGCCGATCCTGACCAGGGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACATATTGCGTGCACCACAGCGTTGACTgggg                                                                                                   <  3:40888/135‑1 (MQ=255)
                              tAGCCACGCCGATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTgggggg                                                                                                 >  4:80358/1‑135 (MQ=255)
                               aGCCACGCCGATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGt                                                                                                >  5:260771/1‑135 (MQ=255)
                                  cACGCCGATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTgg                                                                                             <  3:151090/135‑1 (MQ=255)
                                        gATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTcc                                                                                       >  8:252489/1‑135 (MQ=255)
                                          tCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACAACAGCGTTGACTGGGGGGTTGGGATTCCCt                                                                                     <  1:50090/135‑1 (MQ=255)
                                             tGACCAGTGTGTTTGTCGCTACTCAGGTGGTGGCGCAGGGCCTGTCACTGGAGCTGGCGGCGCCGCGTACCTTGGTCACGCTGGTGACGTATGGCGTGCACCACAGCGTTGACTGGGGGGTGGGGATTACCTTgg                                                                                  >  1:164698/1‑135 (MQ=255)
                                             tGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTgg                                                                                  >  1:164705/1‑135 (MQ=255)
                                                     gtgtTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCggtgggt                                                                          <  7:252489/135‑1 (MQ=255)
                                                       gtTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTgg                                                                                             >  2:8365/1‑114 (MQ=255)
                                                       gtTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTgg                                                                                             >  6:268387/1‑114 (MQ=255)
                                                       gtTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTgg                                                                                             <  1:8365/114‑1 (MQ=255)
                                                       gtTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTgg                                                                                             <  5:268387/114‑1 (MQ=255)
                                                       gtTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGgtgggtgg                                                                        >  2:4240/1‑135 (MQ=255)
                                                       gtTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGgtgggtgg                                                                        >  3:144906/1‑135 (MQ=255)
                                                           ggCGCTACTCAGGTGGGGGCGCAGGGGCTGTCACTGGCGCGGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCtt                                                                     <  4:204268/134‑1 (MQ=255)
                                                           ggCGCTACTCAGGGGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCtt                                                                     <  4:258368/134‑1 (MQ=255)
                                                                     aGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCa                                                          >  3:59188/1‑135 (MQ=255)
                                                                      ggtggtGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAg                                                         <  8:146540/135‑1 (MQ=255)
                                                                      ggtggtGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAg                                                         <  3:80358/135‑1 (MQ=255)
                                                                          gtggCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTgg                                                     >  6:201101/1‑135 (MQ=255)
                                                                           gggCGCAGGGGCTGTCACTGGCGCGGGCGGCGCCGAGTACCTTGGTCACGCGGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTggg                                                    <  2:287810/134‑1 (MQ=255)
                                                                             gcgcAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGgt                                                  >  1:231811/1‑135 (MQ=255)
                                                                             gcgcAGCGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGAGTGGGGGGTTGGGAGTCCCGTGGCGGTGGGTGGCTTGCTCCGCATCAGCTGGGgt                                                  >  4:4659/1‑135 (MQ=255)
                                                                                cAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGtgtg                                               <  4:60758/135‑1 (MQ=255)
                                                                                cAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGtgtg                                               <  5:201101/135‑1 (MQ=255)
                                                                                            ctggcgctggcgGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCAt                                   >  3:86639/1‑135 (MQ=255)
                                                                                                  ctggcgGCGCCGAGTACCTTGGTCACGCTGGTGCCCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCGTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTg                             >  7:293703/1‑135 (MQ=255)
                                                                                                    ggcggcGCCGATTACCTGGGTCACGCTGGTGACCTATGGCGTGGACCACAGCGTTGACTGTGTGGTTGGGATTCCCTTGGCGGGGGGTGGCGTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGcc                           <  2:164698/135‑1 (MQ=255)
                                                                                                      cggcgCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGcc                           <  2:164705/133‑1 (MQ=255)
                                                                                                               gTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGGTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGGGGCTTGCTCAGCATCAGCTGGGGTGGGAAGCTGGCCCATGCGTTGCCGGAGAAGGTAt                <  5:125763/135‑1 (MQ=255)
                                                                                                                    ttGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCgg           <  7:99931/135‑1 (MQ=255)
                                                                                                                    ttGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCgg           <  1:276312/135‑1 (MQ=255)
                                                                                                                        tCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGa       >  5:105786/1‑135 (MQ=255)
                                                                                                                        tCACGCTAGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCggtgggt                                                                          <  1:228372/68‑1 (MQ=255)
                                                                                                                        tCACGCTAGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCggtgggt                                                                          <  1:232283/68‑1 (MQ=255)
                                                                                                                        tCACGCTAGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCggtgggt                                                                          >  2:228372/1‑68 (MQ=255)
                                                                                                                        tCACGCTAGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCggtgggt                                                                          >  2:232283/1‑68 (MQ=255)
                                                                                                                         cACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGAt      <  1:4240/135‑1 (MQ=255)
                                                                                                                             cTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTc  <  7:32138/135‑1 (MQ=255)
                                                                                                                               |                                                                                                                                    
GCGGCTTGTTCGGGGTGGGTGGGGCCGTGGTAGCCACGCCGATCCTGACCAGTGTGTTTGGCGCTACTCAGGTGGTGGCGCAGGGGCTGTCACTGGCGCTGGCGGCGCCGAGTACCTTGGTCACGCTGGTGACCTATGGCGTGCACCACAGCGTTGACTGGGGGGTTGGGATTCCCTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTC  >  KT2440/2076846‑2077105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑