Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,084,843 C→T 7.1% A371V (GCG→GTG)  deaD → ATP‑dependent RNA helicase DeaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,084,8430CT 7.1% 144.0 / 3.5 56A371V (GCG→GTG) deaDATP‑dependent RNA helicase DeaD
Reads supporting (aligned to +/- strand):  ref base C (30/22);  new base T (2/2);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01

AGTCCTACGTACACCGTATCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGTTCGACCGCCTGACCACCGACCTG  >  KT2440/2084711‑2084970
                                                                                                                                    |                                                                                                                               
aGTCCTACGTACACCGTATCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAgcgc                                                                                                                               >  7:181905/1‑135 (MQ=255)
 gTCCTACGTACACCGTATCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAgcgcg                                                                                                                              <  6:27943/135‑1 (MQ=255)
       cGTACACCGTATCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCCTGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGc                                                                                                                         >  8:202344/1‑134 (MQ=255)
        gTACACCGTATCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGc                                                                                                                         <  3:81617/133‑1 (MQ=255)
               gTATCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGccc                                                                                                                <  6:278151/135‑1 (MQ=255)
               gTATCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGc                                                                                                                  >  8:98952/1‑133 (MQ=255)
                 aTCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAg                                                                                                              >  4:291908/1‑135 (MQ=255)
                 aTCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAg                                                                                                              >  2:58845/1‑135 (MQ=255)
                 aTCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGGTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAg                                                                                                              >  1:195503/1‑135 (MQ=255)
                   cGGCCGTACTGGCCGTGCCGGTCGCGATGGGCGGGCCCGGTTGCGGGTTTCCCCGCGTGAGCGCCGCATGGTGCCGGGGATAGAGCGGGTTTCCGGGGAGGAGGTTGCCGAACCGCGCCGGCCGACTGcccccgc                                                                                                            >  2:153882/1‑131 (MQ=255)
                   cGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAgg                                                                                                             <  6:182746/134‑1 (MQ=255)
                   cGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGAGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGc                                                                                                            >  8:105678/1‑135 (MQ=255)
                    ggCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGcc                                                                                                           >  2:160285/1‑135 (MQ=255)
                    ggCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGcc                                                                                                           >  7:59041/1‑135 (MQ=255)
                    ggCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTCCCGAAGCGCGCCTGCCGAATGCCCAGGcc                                                                                                           >  4:211876/1‑135 (MQ=255)
                        gTACTGTCAGTGCCGGTCGCGATGGCCGTGCACTTTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTTCAGGTGATCGAGCGTGTTACCGGGCAGAATGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGc                                                                                                       <  2:12527/135‑1 (MQ=255)
                             ggCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGAt                                                                                                  >  3:250226/1‑135 (MQ=255)
                               ccGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGAt                                                                                                  <  8:30462/133‑1 (MQ=255)
                               ccGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGAt                                                                                                  <  3:291908/133‑1 (MQ=255)
                               ccGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGc                                                                                                <  3:119326/135‑1 (MQ=255)
                                 gTGCCGGTCGCGATGGCCGTGCCCAGTTGCGGGGGACCCCCCCGGCCCGCCGCAAGCTGGACGTGATCGAGCCTGTTACCCGGGAGAACGTTTCCGAAGCCCCCCGGCCGCAGTCCCCCGCCCGGCTGGATGccc                                                                                              >  4:6143/1‑135 (MQ=255)
                                      ggTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATc                                                                                         <  4:58887/135‑1 (MQ=255)
                                                    tGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCt                                                                            >  8:291929/1‑134 (MQ=255)
                                                       aCTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGACTGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAg                                                                        >  6:39235/1‑135 (MQ=255)
                                                            tGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTg                                                                    <  8:181905/134‑1 (MQ=255)
                                                              cTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATc                                                                                         <  6:212078/111‑1 (MQ=255)
                                                              cTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATc                                                                                         >  5:212078/1‑111 (MQ=255)
                                                                      cccGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTg                                                           >  1:54744/1‑133 (MQ=255)
                                                                      cccGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTg                                                           >  8:35039/1‑133 (MQ=255)
                                                                      cccGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTg                                                           >  5:285944/1‑133 (MQ=255)
                                                                        cgcgTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGcc                                                                                                           >  6:207495/1‑83 (MQ=255)
                                                                        cgcgTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGcc                                                                                                           <  5:207495/83‑1 (MQ=255)
                                                                        cgcgTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAg                                                       >  7:146272/1‑135 (MQ=255)
                                                                        cgcgTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAg                                                       <  7:135415/135‑1 (MQ=255)
                                                                             gAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGaa                                                  >  7:204197/1‑135 (MQ=255)
                                                                              aGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGccc                                                                                                                <  8:84874/72‑1 (MQ=255)
                                                                              aGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGccc                                                                                                                >  7:84874/1‑72 (MQ=255)
                                                                              aGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGaa                                                  >  5:4542/1‑134 (MQ=255)
                                                                                   cgcATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAgca                                            >  3:222979/1‑133 (MQ=255)
                                                                                        gctgcAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGcccc                                       >  3:54707/1‑133 (MQ=255)
                                                                                          tgcAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCTCTGGTAGCCGAAGCAGAAGccaccc                                     <  7:202344/135‑1 (MQ=255)
                                                                                             aGGTCATCGTGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGGGCGGGATGCCCGCATCAAGAAGCTGACCTCCAGCCGGGCGCCGCTGGTAGCCTAAGCAGAAGCCACCCACg                                  <  1:240298/135‑1 (MQ=255)
                                                                                                     gAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCTTCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTg                          >  7:201031/1‑135 (MQ=255)
                                                                                                       gCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGtt                        >  4:111084/1‑135 (MQ=255)
                                                                                                         gtgtTACCGGGCAGAAGGTTGCCGAAGTGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGc                                                                                                >  1:84326/1‑61 (MQ=255)
                                                                                                         gtgtTACCGGGCAGAAGGTTGCCGAAGTGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGc                                                                                                <  2:84326/61‑1 (MQ=255)
                                                                                                         gtgtTACCGGGCAGAAGGTTGCCGAAGTGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGc                                                                                                <  2:88504/61‑1 (MQ=255)
                                                                                                         gtgtTACCGGGCAGAAGGTTGCCGAAGTGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGc                                                                                                >  1:88504/1‑61 (MQ=255)
                                                                                                         gtgtTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCA‑CCACGGCGAGCTGTTCGa                     >  1:220276/1‑135 (MQ=255)
                                                                                                             aaCCGGCCAGAAGGTTGCCGACGCGCGCAGGCCTAAGCCCCAGGCCGTGCTTGATGCCCTCATAAAGAAGGTGACCTCGTGCCGGGCGCGGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGTTCGACCg                  <  2:90020/134‑1 (MQ=255)
                                                                                                                  ggCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGTTCGACCGCCTGa             <  7:21738/135‑1 (MQ=255)
                                                                                                                  ggCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGTTCGACCGCCTGa             <  8:59041/135‑1 (MQ=255)
                                                                                                                       aaGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGTTCGACCGCCTGaccacc        <  2:195503/135‑1 (MQ=255)
                                                                                                                       aaGGGTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGTTCGACCGCCTGaccacc        <  4:78486/135‑1 (MQ=255)
                                                                                                                        aGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGTTCGACCGCCTGACCACCg       >  6:235530/1‑135 (MQ=255)
                                                                                                                              ccGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCTTCAAGAAGCTGACCTCGAGCCTTGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGTTCGACCGCCTGACCACCGACCTg  <  8:201031/134‑1 (MQ=255)
                                                                                                                                gAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCaagaa                                                                                    <  1:181539/50‑1 (MQ=255)
                                                                                                                                gAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCaagaa                                                                                    >  2:181539/1‑50 (MQ=255)
                                                                                                                                    |                                                                                                                               
AGTCCTACGTACACCGTATCGGCCGTACTGGCCGTGCCGGTCGCGATGGCCGTGCACTGTTGCTGGTTACCCCGCGTGAGCGCCGCATGCTGCAGGTGATCGAGCGTGTTACCGGGCAGAAGGTTGCCGAAGCGCGCCTGCCGAATGCCCAGGCCGTGCTGGATGCCCGCATCAAGAAGCTGACCTCGAGCCTGGCGCCGCTGGTAGCCGAAGCAGAAGCCACCCACGGCGAGCTGTTCGACCGCCTGACCACCGACCTG  >  KT2440/2084711‑2084970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑