| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,491,272 | A→C | 44.4% | H33P (CAC→CCC) | iutA → | Ferric aerobactin receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,491,272 | 0 | A | C | 44.4% | 1.4 / 3.7 | 22 | H33P (CAC→CCC) | iutA | Ferric aerobactin receptor |
| Reads supporting (aligned to +/- strand): ref base A (8/4); new base C (2/8); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACC > KT2440/2491161‑2491405 | cAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGGTGTGTTGACCAAGCCGTGATGTGTTGGACTGCCGTGGCCCGGTTGGTCGGCCCCCCGCACGCCGGGGCCGCGCCCGCGGCCg > 1:248722/1‑135 (MQ=255) tCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCGTTTCACCATGCCGTGATGTCTTTCAGTGCCGTGGCCCTGTTGCTCGGCCCCCAGCACCCAGGGGCCGCGACGGCTGCCGCACCGcccccca > 6:68766/1‑132 (MQ=255) tcctGCACACGCCCTGCCCCCGGCGTCGCGTTTATTTACCCCATGCCTTGTTGTGTGGCTGGGCGGGGGCCCGGTGGCGCGCCCCCCGCCCCGCGGGGCCCGCGACGGCTGCCGAACAGACCCAGATGCAGGccc < 6:205876/132‑1 (MQ=255) tGAGGCCCAAGCCCTTCCCCCGGCGGGGGGGTTTTTTACCCCATGCCTTGATGGGTTGCTGTGCGGTGCACGTGTGGCGCGCCCCCGTGCACGCCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGccc < 6:246145/135‑1 (MQ=255) gaggCTCAAGCCGTTGCCCCGCGGGGGGGGTTCTTTTCCCCATGCCTTGTGGTCTTGCTGGGCCGGGGCCGGGTGGCGCGGCCCCCTGCCCGCAGGGGCCGCGACTGCTGCCGAACAGACCCAGATGCAGGcc < 8:139460/130‑1 (MQ=255) gCCCTTGCACCGCCGTCGCGGTTGATTTGCCCATGCCATGATGTCTTGCAGTGCCGTGGCCCAGTTTCTACGCCCCCTGCCCGCACGGGCCGCCCCGGCTGCCGCACAGACCCAGAAGCAGGCCCCCCACGtacc > 8:158254/1‑132 (MQ=255) ggggcgGTTTTTTCCACCTGCCCTTGCTGGCTGGCGGGGCGGGGCCCGTGGGGCGCGCCCCCCGCCCCGCGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAg < 5:120223/132‑1 (MQ=255) ggcggggTTCTTTTCACCATCCTTGGGTTGCTGCCGGGGCGGGGGCCCTGGTGCCGCCCCCCCCGCACCCGGGGCCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAg < 3:100161/130‑1 (MQ=255) tCACCATGCCTGGATGTCTTCCTGGGCGGGGGCCGTGTTGGTCGGCCCCCTCCACGCTGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTgg < 6:154653/134‑1 (MQ=255) gCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGgcg > 4:104044/1‑135 (MQ=255) cGTGGCCCTGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGCCCCAGATGCAGGCCCGCCAGGTCCCGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCCCTTTCGCCGCCCa > 8:161253/1‑135 (MQ=255) cccTGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGCCCACTTTCGCCGCCCCggccg > 5:269540/1‑135 (MQ=255) tgtgGCGCGCCCCCCTCCCCGCCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccggcc < 5:68766/135‑1 (MQ=255) gtGCGCGGCCCCCCGCCCGCGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTg < 3:22624/134‑1 (MQ=255) ggcgcGGCCCCCTGCCCGCCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGc < 8:245282/131‑1 (MQ=255) gCTCGGCCCCCTGCCCGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGCTGCAGGCCCGCCAGGTCCAGCTCGCCCTGCCAGCCCAACCGCTGGACCAGGCGCGGACCACTTTCTCCGCCCCCGCCCGCCGGCa > 8:158916/1‑135 (MQ=255) gCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGCCCTGCCAGCCCACCCGCGGGACCAGGCGCTGACCACTTTCGCCGCCCCGGCCGGCCTGCa > 5:75664/1‑135 (MQ=255) tCGGCCCCGGCCACGCTGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCAc < 7:158254/134‑1 (MQ=255) gCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCCACCGCTGGACCAGGCGCTGACCCCTTTCGCCGACCAGCCCGGCCTGCACctgc > 4:226822/1‑135 (MQ=255) cccccTGCCGCCAGGGCCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACctgct < 1:173711/135‑1 (MQ=255) cccccTGCCCGCGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACctgct < 3:203652/135‑1 (MQ=255) ccTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGCCCACTTTCGCCGCCCAGGCCGGCCTGCACCTGCTGTa > 5:125897/1‑135 (MQ=255) aCGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCCACCGCTGGACCAGGCGCGGCCCACTTTCGCCGACCAGGCCGGCCTGCACCTGCAGTAcacc > 4:13352/1‑134 (MQ=255) | CAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACC > KT2440/2491161‑2491405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |