| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 96,480 | T→G | 33.4% | intergenic (+121/‑72) | cynT → / → CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 96,480 | 0 | T | G | 33.4% | 31.9 / 3.4 | 39 | intergenic (+121/‑72) | cynT/CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (11/13); new base G (8/5); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGT > KT2440/96348‑96600 | ggCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGgc > 8:253345/1‑135 (MQ=255)ggCAGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgc < 2:261159/135‑1 (MQ=255) cGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTGGGGGGCCGTGAATTCCCTGCCAGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCCgggcg > 2:251568/1‑135 (MQ=255) ctactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGGCGTCGGCTGCCGTGAATTCCCAGACAGCCCAGCCCGCCTGGACGCTGGCGGCCCGCCCCGgggcgcg > 3:86686/1‑134 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCtt < 3:92885/135‑1 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTGGGGGGGCGTGTATTAGCTGGCGGGCCAGGCGGCGGGGTGGCCGGCGGGCCGCGCCGgggcgcgg > 5:213918/1‑133 (MQ=255) ctaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCGTg > 8:251709/1‑134 (MQ=255) gACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCTTGTCAgggg > 7:88215/1‑135 (MQ=255) gACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCGGCCTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGAGGCGGCGCGCGTGGCCgggg > 8:18651/1‑135 (MQ=255) cccGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTTGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGc > 7:75773/1‑134 (MQ=255) cccGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCa < 4:167113/135‑1 (MQ=255) cGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGCATTCCCTGTCTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCCCCTGCGCGCGTGTCCGGGGCaac > 8:104193/1‑134 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTAGCTGGCGGCCCGCGCCGGCGCGCGTGTCAGGGGCAAAc > 4:243569/1‑135 (MQ=255) ccgccgCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGGGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGt < 6:239994/135‑1 (MQ=255) acaaCCTTGATCGCCGGCGGCACCCTTCAGGGGTGGGGGGCGGCCTTCGGCTGCCTTGAATGCCCTGCCTGCCCGGCGGGCGGGCGCGCGGGCGGCCCGCGCCGGCGGGCTTGTCCGGGGCAAACGTGCCCACg < 8:259814/132‑1 (MQ=255) aGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGAGCCCCAGGCCCGGGGGATg > 8:89274/1‑135 (MQ=255) aGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTACCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCGTGTCAGGGGGAAAAGTGCCCACGGCCAGGGGAATg > 3:104753/1‑135 (MQ=255) aGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTACCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCCCGTGTCAGGGGCAAACGTGCCCACGGCCAGGGGaaag > 8:93317/1‑133 (MQ=255) gCCGGCTGCACCCTTGCGGGGGGGGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCGGCGCGCTTGTCCGGGGCAAACGTCCCCACGGCCAGGGGAATgg < 1:251568/135‑1 (MQ=255) gCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCg > 7:231963/1‑135 (MQ=255) aGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCGGTCTGCCCTGCCGGCGTGCGAGCTGGCGGCCCGCGCCGGCGCGCGTGGGCGGGGCCAACGTGCCCACCGCCCGGGGGATGGCCGGGCGGCGAGAGaa > 4:115576/1‑135 (MQ=255) gcggGGCTTGCGGCTGCCTTTAATTCCCTGCCTGCCCTGCCGGGGTGCGCGCGGGGGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAAGGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAac < 2:269226/135‑1 (MQ=255) ggggCGTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCGGGCGTGCTCGCTGGCGGCCCGCGCGTGCGCGCTTGTCAGGGGAAAACATGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:143005/133‑1 (MQ=255) gcgGCCTTGGGCTGCCTTTAATTCCCTGACTGCCCGGCCGGCGTGCGCGGGGGCGGCCCGCGCCTGGGCGGTTGTCCGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 5:291498/135‑1 (MQ=255) gcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 8:231963/135‑1 (MQ=255) gcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 6:50571/135‑1 (MQ=255) gcgGCCTGCGGGTGCCTTTAATCCCCTGACGGCCCTGCGGGCGTGCTCGCGGGGGGCCCGCGCCGGCGCGCTTGTCTGGGGCAAACGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 3:110599/135‑1 (MQ=255) gcgGCAGTCGGTGGCCTTTAATTCCCTGCCTGCCCTGCGGGGGTGGTGGCGGGCGGCCGGCGCGTGGGCGTTTGTCAGGGTAAAACGGCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 2:235108/135‑1 (MQ=255) ttCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCCCCCCTGCGCGCGTGGAAGGGGCAAACCTGCCCACCGCCCCGGGGATTGCCGTGCGACGAGAGAAAAGAGAAACACAGTGa > 4:259081/1‑134 (MQ=255) gtCGGGTGCCTTGAATTACCTGACTGCCCTGCCCGCGTGGGCGCGGGCCGCCCGCGGCAGCGCGCTTGTCAGGGGCAAAACAGACCACGGCCAAGGGTAATGCCGGTCGCCCTGAGAAAAGACAAACACCGTGaa > 8:206955/2‑135 (MQ=255) gctgctTTTAATTCCCTGACTGCCCTGCCGGCGTGCGCGGTGGCGGCCGGCGCCGGCGGTGTTGTCAGGGTAAAACGTCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 1:7590/135‑1 (MQ=255) tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:198496/118‑1 (MQ=255) tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac > 8:198496/1‑118 (MQ=255) tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGt > 2:192977/1‑135 (MQ=255) ccTGACGGCCCTGCCGGCGTGCTCGGGGGCGGCCCGCGCCGGGGCGCTTGTCCGGGGCAAACGGCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGc < 8:75773/135‑1 (MQ=255) ccctgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGGATGGCCGTGCGACGAGAGCAAGGAGAAACACGGTGCACATTACACAGTTGAAAGCGGCCCTGc > 2:162888/1‑135 (MQ=255) ccctgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACCGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGcccggg > 7:149655/1‑132 (MQ=255) cGCTGGCGGCCGGCGCCTGCGGGGTTGGCTGGGGAAAACGTGCCCACGGCCAGGGGAATGGCGGTGCTAATAGAGAACGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTgg < 4:73503/135‑1 (MQ=255) tGGCGGCCCGCGCCTGCGCGCTTGGCAGGGGCAAACGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGt < 2:31109/135‑1 (MQ=255) | GGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGT > KT2440/96348‑96600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |