| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,642,546 | G→A | 11.8% | A136T (GCC→ACC) | ynhG → | putative L,D‑transpeptidase YnhG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,642,546 | 0 | G | A | 11.8% | 87.2 / 4.5 | 34 | A136T (GCC→ACC) | ynhG | putative L,D‑transpeptidase YnhG |
| Reads supporting (aligned to +/- strand): ref base G (18/12); new base A (2/2); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01 | |||||||||||
GCGAAGGCATCGTCATCAACCTGGCCGAGTACCGCATGTACTACTTCCCCAAGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCGACTGTGGTGCCTGCTGGCCCGGACAACCCGCTGGGG > KT2440/2642430‑2642677 | gcgAAGGCATCGTCATCAACCTGGCCGAGTACCGCATGTACTACTTCCCCAAGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGAGCGCCAACACCAGGATTAcag > 3:219237/1‑133 (MQ=255) tcatccACCTGGCCGCGTACCGCATGTACTACTTCCCCAAGGGGGAGAGCGGGGTGTACACCTTCCCGCTGGGCATTGGTCGCGATGGGTGGGGGTCTCCGATCGCCAACACCAGGATTACCGCCAAGACGccc < 5:31433/134‑1 (MQ=255) catcaACCTGGCCGAGTACCGCATGTACTACTTCCCCAAGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCaa > 8:204404/1‑135 (MQ=255) cGAGTACCGCATGTACTACTTCCCCAAGGGGGAGAGCGTGGTGTACACCTTCCCGCGGGGCATTGGTCGCGAGGGGGGGGGGTCGCCGCTCGGCAACACCAGGTTTACCGCCCAGGCGCCCCACCCGCCCTggcc > 1:153819/1‑133 (MQ=255) actactTCCCCAAGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCCAGCCGCCCCCACCGCCCTGGCAGCCGCCCGCGTCGa > 4:154414/1‑135 (MQ=255) ctactTCCCCAAGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACCCCAGGATTACCGCCAAGCCGCCCAACCCGCCCTGGACGCCGCCCGCTTCGag > 3:197753/1‑134 (MQ=255) cccATGGGGGAGAGCGTGTGGTACCCCTTCCCGGGGGGCATTGGTCGCGTGGGGGGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCaaa < 2:102892/135‑1 (MQ=255) cccAAGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGc > 5:212194/1‑132 (MQ=255) cccAAGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCaaa > 1:195104/1‑135 (MQ=255) aGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGAc < 6:79659/91‑1 (MQ=255) aGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGAc > 5:79659/1‑91 (MQ=255) gggggAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGCCCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCa > 8:11579/1‑135 (MQ=255) gagGGTGGTTTACACCTTCCCGCGGGGCATTGGTCGGGCGGGGGGGGGGGCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGAATCGCAAAGAGCACGCCGa < 7:17307/135‑1 (MQ=255) gCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTGGCCGCTCGCCAACCCCAGGATTCCCGCCAAGTAGCCCCAACCCACCGGGCCGCCGCCCGCGTCGCATCGCAAAGCGCCCGCCGAgg > 2:295575/1‑135 (MQ=255) gtACACCGTCCCGGTGGGCTTTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCAAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGa < 3:283794/135‑1 (MQ=255) cccTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTGCCCCATCGCCAACCCCAGGATTACCGCCAAGACGCCCCACCCGACCTGGACGCCGCCCGCGGCGCTTCGCAAACACCCCGCCGCGGACGGTGCCagc > 1:99555/2‑133 (MQ=255) cTTCCCGCGGGGCATTGGGCGCTAGGGGGGGGGGGCGCCTATCGCCAACACCAGGATTACCGCAAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCt < 2:166307/135‑1 (MQ=255) ttCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCACGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTg > 3:130481/1‑135 (MQ=255) gCTGGGGATTGGTCGCTAGGGGTGGGGGTCGCCGATCGCCAACACAAGGATTACCGCCAAGACGCCCACCCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCgcc > 1:183637/1‑133 (MQ=255) gggctgtGGTCGCGCGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCGACtgt < 8:45183/129‑1 (MQ=255) aTTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCGACTgtggtg > 7:12607/1‑135 (MQ=255) cgcgAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGAcgcc > 7:159465/1‑71 (MQ=255) cgcgAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGAcgcc < 8:159465/71‑1 (MQ=255) cgAGGGGTGGGGGGCGCCTATCGCAAACACCAGGATTACCGCCAAGACCCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCGACTGTGGTGCCTGCTgg < 7:24480/135‑1 (MQ=255) aGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCGGCCGTCGGTCGTGCCTGCTGGcc > 1:280451/1‑135 (MQ=255) aGGGGTGGGGGTCGCCGATCACCAACACCAGGATTACCGCCAAGCCGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCGACTGTGGTGCCTGCTGGcc > 5:173715/1‑135 (MQ=255) ggggggggCGCCGCTCGCCAACACCAGGATTACCGCCAACACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAACGAGCACGCCGAGGACGGTGACATCCTGCCGACTGTGGTGCCTGCTGGCCCGGa < 5:130595/133‑1 (MQ=255) gTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCGACTGTGGTGCCTGCTGGCCCGGa < 7:204404/135‑1 (MQ=255) gggTCGCCGATCACCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGAcgc < 2:215081/58‑1 (MQ=255) gggTCGCCGATCACCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGAcgc > 1:215081/1‑58 (MQ=255) gCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCGACTGTGGTGCCTGCTGGCCCGGACAACCCGCt < 4:197753/135‑1 (MQ=255) gCCGATCACCAACCCCAGGATTACCGCCACCACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCGACTGTGGTGCCTGCTGGCCCGGACAACCCGCt < 6:173715/135‑1 (MQ=255) gATCGCAAACACCATTATTACCGCAAAGACTCCCAACCCCACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCCAGCCCGCTCACATCCAGCCGACTCTGGTGCATGCTGGCCCGGACAACCCGCTgcg > 8:96771/1‑133 (MQ=255) aTCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGACGACGGTGACATCCTGCCGACTGTGGTGCCTGCTGGCCCGGACAACCCGCTgggg > 5:15273/1‑135 (MQ=255) | GCGAAGGCATCGTCATCAACCTGGCCGAGTACCGCATGTACTACTTCCCCAAGGGGGAGAGCGTGGTGTACACCTTCCCGCTGGGCATTGGTCGCGAGGGGTGGGGGTCGCCGATCGCCAACACCAGGATTACCGCCAAGACGCCCAACCCGACCTGGACGCCGCCCGCTTCGATTCGCAAAGAGCACGCCGAGGACGGTGACATCCTGCCGACTGTGGTGCCTGCTGGCCCGGACAACCCGCTGGGG > KT2440/2642430‑2642677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |