Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,024,446 C→T 10.5% Y216Y (TAC→TAT mgtB → Magnesium‑transporting ATPase, P‑type 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,024,4460CT10.5% 108.0 / 3.1 38Y216Y (TAC→TATmgtBMagnesium‑transporting ATPase, P‑type 1
Reads supporting (aligned to +/- strand):  ref base C (17/17);  new base T (2/2);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGTGGCCGGCGACATCGTGCAGCTTTCTGCAGGTGACATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATGGGGACCAATGTGGTCAGTGGCCGCGCCCGCGCCGTGGT  >  KT2440/3024314‑3024574
                                                                                                                                    |                                                                                                                                
aCTGGTGGCCGGCGACATCGTGCAGCTTACTGCAGGTGACATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGCCCTGTATATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGa                                                                                                                                >  2:294118/1‑135 (MQ=255)
                aTCGTGCAGCTTTCTGCAGGTGACATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTc                                                                                                                <  7:48004/135‑1 (MQ=255)
                          tttCTGCAGGTGACATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGt                                                                                                      <  4:187640/135‑1 (MQ=255)
                            tCTGCAGGTGACATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTcc                                                                                                    >  2:269946/1‑135 (MQ=255)
                              tGCAGGTGACATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTccgc                                                                                                  >  4:72307/1‑135 (MQ=255)
                                 aGGTGACATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGCAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTccgccgc                                                                                               >  3:46681/1‑135 (MQ=255)
                                 aGGTGACATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTccgcc                                                                                                 <  3:57987/133‑1 (MQ=255)
                                    ggACAGGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGa                                                                                            <  6:169397/134‑1 (MQ=255)
                                      aCATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGac                                                                                                                               <  1:55958/98‑1 (MQ=255)
                                      aCATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGac                                                                                                                               >  2:55958/1‑98 (MQ=255)
                                      aCATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCAGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGc                                                                                          >  2:262268/1‑135 (MQ=255)
                                         tGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCTCCGCCGAGCATg                                                                                       <  6:289870/135‑1 (MQ=255)
                                         tGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATg                                                                                       <  5:142559/135‑1 (MQ=255)
                                                cgccgATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGcaca                                                                                >  5:74224/1‑135 (MQ=255)
                                                  ccgATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATc                                                                              >  1:110983/1‑135 (MQ=255)
                                                                             aCCTGTTAATCAGCCAGGCCGTGCTCACCGCCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGACCGCCGCCGAGCAAGGCGCACATCAGGACAACCTGCTCGAGTAGCCGAACa                                                   >  3:224780/1‑135 (MQ=255)
                                                                               cTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATc                                                 <  4:107458/135‑1 (MQ=255)
                                                                                    tATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCt                                             >  5:104275/1‑134 (MQ=255)
                                                                                     aTCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCtt                                            <  7:132403/134‑1 (MQ=255)
                                                                                      tCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTc                                           <  4:190349/134‑1 (MQ=255)
                                                                                         gCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCTCAGAAGTCCGCCGCCGATCATGGCGCAGATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATgg                                       <  1:262268/135‑1 (MQ=255)
                                                                                         gCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATgg                                       <  3:148592/135‑1 (MQ=255)
                                                                                           cAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATgggg                                     >  4:71012/1‑135 (MQ=255)
                                                                                             ggCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTATGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAgg                                                                           >  2:122695/1‑95 (MQ=255)
                                                                                             ggCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTATGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAgg                                                                           >  2:118954/1‑95 (MQ=255)
                                                                                             ggCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTATGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAgg                                                                           <  1:122695/95‑1 (MQ=255)
                                                                                             ggCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTATGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAgg                                                                           <  1:118954/95‑1 (MQ=255)
                                                                                             ggCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCCTGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATGGGGa                                    >  6:254478/1‑134 (MQ=255)
                                                                                             ggCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATGGGGa                                    >  7:28358/1‑134 (MQ=255)
                                                                                                     tCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATGGGGACCAATGTgg                           <  3:72307/135‑1 (MQ=255)
                                                                                                            cGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATg                                                                                       <  2:30325/68‑1 (MQ=255)
                                                                                                            cGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATg                                                                                       >  1:30325/1‑68 (MQ=255)
                                                                                                               aGCGTTGCCGGTCGAAAAGTACGACAGCTTGGGTAATGTCGCGCAGCAGTCCGCCGCCGAGCATGGCGCACATCAGGCCACCCTGCTCGAGTTGCCGAACATCTGCTTCATGGGGACCAATGTGGTCAGTGGccg                 >  8:224199/1‑135 (MQ=255)
                                                                                                               aGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATGGGGACCAATGTGGTCAGTGGccg                 >  4:213109/1‑135 (MQ=255)
                                                                                                                    tGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATGGGGACCAATGTGGTCAGTGGccgcgccc            >  4:204245/1‑135 (MQ=255)
                                                                                                                       cGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATGGGTACCAATGGGGTCAGTGGccgcgcccgc          <  6:229582/134‑1 (MQ=255)
                                                                                                                              caaGCACGACCCCTTGGTTAATGTCGCGCTGAAGTCCGCCGCCGAGCATGGCGCACAGCAGGTCAACCTGCTCGAGTTGCCGAACAGCTGCTGCATGGGGACCAAGGTGGTCAGTGGCCGCGCCCGCGCCgtggt  <  1:294118/134‑1 (MQ=255)
                                                                                                                              aaaGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATGGGGACCAATGTGGTCAGTGGCCGCGCCCGCGCCgtggt  <  1:158880/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                
ACTGGTGGCCGGCGACATCGTGCAGCTTTCTGCAGGTGACATGATTCCCGCCGATATCCGTCTGCTCGAAGCGCGCGACCTGTTTATCAGCCAGGCCGTGCTCACCGGCGAAGCGTTGCCGGTCGAAAAGTACGACACCTTGGGTAATGTCGCGCAGAAGTCCGCCGCCGAGCATGGCGCACATCAGGACAACCTGCTCGAGTTGCCGAACATCTGCTTCATGGGGACCAATGTGGTCAGTGGCCGCGCCCGCGCCGTGGT  >  KT2440/3024314‑3024574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: