Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,061,793	G→A	100%	S592N (AGC→AAC)	qedA_2 →	Quinoprotein alcohol dehydrogenase (cytochromec)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,061,793	0	G	A	100.0%	89.6 / NA	30	S592N (AGC→AAC)	qedA_2	Quinoprotein alcohol dehydrogenase (cytochromec)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/11);  total (19/11)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGT  >  KT2440/3061661‑3061924                                                                                                                                     |                                                                                                                                    tttCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCGGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                   <  6:229981/135‑1 (MQ=255) tttCGGTAGTCTCCGGCGGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGGCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                   <  5:82944/135‑1 (MQ=255)      gTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAg                                                                                                                              >  5:278212/1‑135 (MQ=255)       tAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAg                                                                                                                              >  7:284770/1‑134 (MQ=255)         gTCTCCGGCTGGGGCGTCGCGGTGGCGCTGTGGGTCGGCGAAGTGGCCAAACGGGTCATCGACTTCAACCAGGGCGTCATGCTCTGGACCTTCCAGTTCCCCATGCAGTTGCAGCAAACGTCAAACGTCACGCCa                                                                                                                           <  4:66179/135‑1 (MQ=255)          tctaCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCa                                                                                                                           >  8:54374/1‑134 (MQ=255)               ggCGGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACg                                                                                                                      <  8:120821/134‑1 (MQ=255)                                   gCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtgctg                                                                                                 <  1:171067/135‑1 (MQ=255)                                        ggggCGGCGAAGTGGCCAAACGGGGCAAGGACTTCAACCAGGGCGGCATGCGCTGGACCTTCCAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                            <  8:76760/135‑1 (MQ=255)                                            cggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATc                                                                                        >  5:136098/1‑135 (MQ=255)                                                  aGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                        <  1:283202/97‑1 (MQ=255)                                                  aGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                        >  2:283202/1‑97 (MQ=255)                                                       ccAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCg                                                                             >  4:208887/1‑135 (MQ=255)                                                         aaaCGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGc                                                                           >  8:248285/1‑135 (MQ=255)                                                              gttCAAGTACTTCACCCCGGGCGGCTTGGTCTGGACCTTAAAGTTGCCCAAGCAGTTGCAGAAAACGGAAAACGTCAAGCAAAACCCCCTCCATACTGCCTGTTGCTGCCGGTGAGGGGTGGAAAGCGGCCctac                                                                      <  8:38069/133‑1 (MQ=255)                                                                 cAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCctacta                                                                    >  5:284316/1‑134 (MQ=255)                                                                   aGGACTTCAACCCGGGCGGCATGGTTTGTACCTTCAATTTCCCCAAGCCTTTGCCCAAAACCGCAAACGTCAAGCCAAAACCCCGGCCTACTGCCGGTTGGGGCCGGTTTTGGGGGGCAAGCGGCCCTACTACCa                                                                 <  2:30345/135‑1 (MQ=255)                                                                            aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACg                                                          >  8:209750/1‑133 (MQ=255)                                                                            aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACg                                                          >  7:255786/1‑133 (MQ=255)                                                                             ccAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACgaga                                                       >  8:76008/1‑135 (MQ=255)                                                                                  gcggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGcccc                                                  >  5:102344/1‑135 (MQ=255)                                                                                   cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCGGATCGGTGGCAAGCCGCCCAACTACCAAATGACAAGACCCCCg                                                 >  5:241038/1‑135 (MQ=255)                                                                                    ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCAGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGc                                                >  1:65119/1‑135 (MQ=255)                                                                                       aTGCTCGGGACCTTCACGTGCCCCACGCTGTTGCACCAACCGCCAAACGTCACGCCATAACGCCTGCCTACTGCCGCTTGCGGCCGCTGAGCGGGGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGc                                             <  2:3565/135‑1 (MQ=255)                                                                                                       aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcc                             >  4:190154/1‑135 (MQ=255)                                                                                                       aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCCGTGGCAACCCGCCCGACAACCAAAAGACCAGAGCCCCCCTGCCAACGAGGCATTAGcc                             >  5:269382/1‑135 (MQ=255)                                                                                                        gTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCGGCCAACGATGCATTCGccc                            >  4:81036/1‑135 (MQ=255)                                                                                                              cacgCAGTTGAACCAACCGCAAAACGTAAACCCATACCGCCTGCCTCCTGCCGCTTGCGGCGGCTGATCGGTGGAAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcg                      <  4:290017/132‑1 (MQ=255)                                                                                                              cacccAGTTCCAGAAAACGGCACACGACACCCCAAACCGCCTCCCTACGCCCGCTGGCGGCCGCTTCTCGGGGCCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcg                      <  6:74341/131‑1 (MQ=255)                                                                                                                agaaGTTCCAGCAACCGGCAAAGGCCAAGCCATAACGCCTGCCTACGGCCGCTGGCGGCCGCTGATCGGTGGAAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgcg                    <  4:290554/132‑1 (MQ=255)                                                                                                                         agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGCTCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGGTGCATTCGCCCGGCACGCGGGGcttctt           >  8:118570/1‑135 (MQ=255)                                                                                                                          gcaaacggcaaacgTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAACGGTGGCAAGCGGCCCTACTACCAAATGACGAGCGCCCCCCTGCCAACGCTGCATTCGCCCGGCCCGCGGGGcgtggtt          >  1:127421/1‑129 (MQ=255)                                                                                                                                  cAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGt  >  7:146837/1‑135 (MQ=255)                                                                                                                                     |                                                                                                                                    TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGT  >  KT2440/3061661‑3061924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑