Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,134,642 G→T 12.1% V168F (GTC→TTC)  CJ019_02952 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,134,6420GT12.1% 82.5 / 3.9 33V168F (GTC→TTC) CJ019_02952hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (9/20);  new base T (2/2);  total (11/22)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGCGTGACGTGATTCCCAACACCGTGCAGGACGCCCGCAGCCAGATCGCCGCGCTCCT  >  KT2440/3134511‑3134775
                                                                                                                                   |                                                                                                                                     
cctCGCAGTACCGCCTCAACAACCCCCCCCCGGACAACGGCGCGGCCGGCCGCGCGGTGGCCGAACAGCTAGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCt                                                                                                                                    <  4:142414/133‑1 (MQ=255)
          cAGCCTCAACAACACCACCTCGGACAACGTCTCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCg                                                                                                                          >  7:205813/1‑135 (MQ=255)
                caacaaCACCACCTCGGACAACGTCGCGGCCGGGCGCGCGTTGGCCGAAAAGCTGGTGGGCGCCAACGGCGGGAATGGCCGCATCCAGGTGTTGAACGGCTTCTACGGGGTGCCGGTCTGTTAGATACGCTaaga                                                                                                                    >  8:128608/1‑135 (MQ=255)
                   caacaCCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCCTCCTGGTGTTCAACGGCTTCTACGGGGTGCCGTTCTGTGCGATCCGCTAcgacga                                                                                                                 >  8:78460/1‑135 (MQ=255)
                   caacaCCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGTTCTGTGCGATCCGCTAcgacga                                                                                                                 >  8:82693/1‑135 (MQ=255)
                      caccccGTCGGACACGGGCGGGGCGGGCGGCGGTTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGtt                                                                                                              <  4:93386/135‑1 (MQ=255)
                            cTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGCCACCGGCGGCAAGGGCCGCCTGCTGGTGTTCAACGGCTTCGACGGGGTGCCGGTCTGTGCGCTCCGCTACGCCGAGTTgaagca                                                                                                        >  4:35299/1‑133 (MQ=255)
                            cTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGCCACCGGCGGCAAGGGCCGCCTCCTGGTGTTGAACGGCTTCTTCGGGGGGTGGGTCTGTGCGATCCGCTACGCCGATTTgaatca                                                                                                        >  6:48612/1‑132 (MQ=255)
                                            gccggccgCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcat                                                                                        <  4:84178/135‑1 (MQ=255)
                                             ccggccgCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTg                                                                                                             <  5:142976/113‑1 (MQ=255)
                                             ccggccgCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTg                                                                                                             >  6:142976/1‑113 (MQ=255)
                                                               aaCAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTcc                                                                     <  8:79348/135‑1 (MQ=255)
                                                                              acacCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtg                                                      <  6:16314/135‑1 (MQ=255)
                                                                               cacCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGAt                                                                          >  2:36768/1‑114 (MQ=255)
                                                                               cacCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGAt                                                                          <  1:36768/114‑1 (MQ=255)
                                                                                cccGGCGGCAAGGGCCTCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  3:189174/134‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGTTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  7:82693/135‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGTTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  7:78460/135‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCATTTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  6:175562/135‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  7:286291/135‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  5:182604/135‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  6:105090/135‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  3:35299/135‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  2:190963/135‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  2:167019/135‑1 (MQ=255)
                                                                                acCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGcgtgac                                                    <  1:42156/135‑1 (MQ=255)
                                                                                        cAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTTCAGATCGTCCAGCCGGAGCTGCGTGACGTGATTcc                                            <  2:268635/135‑1 (MQ=255)
                                                                                          aGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGCGTGACGTGATTCCCa                                          <  3:16098/135‑1 (MQ=255)
                                                                                                     cTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGc                                                         >  4:213406/1‑109 (MQ=255)
                                                                                                     cTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGc                                                         <  3:213406/109‑1 (MQ=255)
                                                                                                                cGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGCGTGACGTGATTCCCAACACCGTGCAGGACGCCCGCAg                    >  4:197919/1‑135 (MQ=255)
                                                                                                                        aCGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGCGTGACGAGATTCCCAACACCGTGCAGGACGCCCGCAGCCAGATcg            >  8:209923/1‑135 (MQ=255)
                                                                                                                          ggggTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCTTCCGCAATTGCAGATCGTCCAGCCGGAGCTGCGTGACGTGATTCCCAACACCGTGCAGGACGCCCGCAGCCAGATcg            <  5:32143/133‑1 (MQ=255)
                                                                                                                                   gTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGCGTGACGTGATTCCCAACACCGTGCAGGACGCCCGCAGCCAGATCGCCGCGCTCCt  >  6:149854/1‑134 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCTGCGTGACGTGATTCCCAACACCGTGCAGGACGCCCGCAGCCAGATCGCCGCGCTCCT  >  KT2440/3134511‑3134775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑