Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,362,995 T→A 5.8% L74Q (CTG→CAG)  CJ019_03161 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,362,9950TA 5.8% 224.5 / 3.6 69L74Q (CTG→CAG) CJ019_03161hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (39/26);  new base A (2/2);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

CCTCCCAGCTTCTGCGACTGGTTGACCAATTCGATGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGACCA  >  KT2440/3362866‑3363127
                                                                                                                                 |                                                                                                                                    
ccTCCCAGCTTCTGCGACTGGTTGACCAATTCGATGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACa                                                                                                                                  >  2:8381/1‑134 (MQ=255)
    ccAGCTTCTGCGACTGGTTGACCAATTCGATGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTAc                                                                                                                              >  1:236451/1‑134 (MQ=255)
              cGACTGGTTGACCAATTCGATGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAAt                                                                                                                   >  6:123210/1‑135 (MQ=255)
               gACTGGTTGACCAATTCGATGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAAt                                                                                                                   <  8:208272/134‑1 (MQ=255)
               gACTGGTTGACCAATTCGATGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAAt                                                                                                                   <  3:149581/134‑1 (MQ=255)
                 cTGGTTGACCAATTCGATGCCACTCTGGCTGAGTTAGGTGGAGTAGTGGCTGTCCGCGATGAGATGCTGAAGCTTCATGCGATGGCCCTAACCCTAGTAGAGGGTGTGCCACTGACAGTACCTACAGACAATGcc                                                                                                                >  4:22897/1‑135 (MQ=255)
                         ccAATTCGATGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCCCTGACAGTACCTACTGAGAATGCCTGCATCt                                                                                                         >  6:203882/1‑134 (MQ=255)
                                 aTGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAgg                                                                                                >  5:167592/1‑135 (MQ=255)
                                 aTGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAgg                                                                                                >  7:80152/1‑135 (MQ=255)
                                     cTGTCTGGCTGAGTTAGTTGTAGTTGTGTCTGTACGAGATGAGCTGCTGAATCTTCATGCGATGTACCTAACCATTTTAGAGGGTGTTCAACTGACAGTACCTACTGAGAATGCCTGCATATGGACGGAGGCTGa                                                                                            >  8:197019/1‑135 (MQ=255)
                                     cTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTg                                                                                             <  2:265205/134‑1 (MQ=255)
                                                 gTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAAc                                                                                 <  3:238349/134‑1 (MQ=255)
                                                   tAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGa                                                                              >  8:88142/1‑135 (MQ=255)
                                                      gTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAAt                                                                            <  4:107985/134‑1 (MQ=255)
                                                             tgtgGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGAc                                                                    <  5:203882/135‑1 (MQ=255)
                                                             tgtgGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGAc                                                                    >  6:145536/1‑135 (MQ=255)
                                                                    gTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACTGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCt                                                             >  6:59045/1‑135 (MQ=255)
                                                                      ccGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCt                                                             <  5:123210/133‑1 (MQ=255)
                                                                       cGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGACTCTCGAGACCCTCGCtggg                                                          >  5:18144/1‑135 (MQ=255)
                                                                       cGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCt                                                             >  5:6256/1‑132 (MQ=255)
                                                                       cGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCt                                                             <  6:6256/132‑1 (MQ=255)
                                                                         agaTGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCtgggtg                                                        <  7:88142/135‑1 (MQ=255)
                                                                              aGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTgc                                                   <  2:90721/135‑1 (MQ=255)
                                                                               gctgctGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTgc                                                   <  7:170213/134‑1 (MQ=255)
                                                                               gctgctGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTgc                                                   <  1:8381/134‑1 (MQ=255)
                                                                                  gctgAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGctc                                               >  2:178778/1‑135 (MQ=255)
                                                                                    tgAAGCTTCATGCGATGGCCCTAAGCCTTGTAGAGGGTGTGCCTCTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGctct                                              <  3:22897/134‑1 (MQ=255)
                                                                                    tgAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGctct                                              <  7:101531/134‑1 (MQ=255)
                                                                                          ttCATGCGATGGCCCTAACCATTTTAGAGGGTGTGCCACTGACAGTACCTACTGAGATTGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAAGAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAg                                       <  6:2271/135‑1 (MQ=255)
                                                                                              tGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTg                                    >  6:191964/1‑134 (MQ=255)
                                                                                              tGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTg                                    >  6:195803/1‑134 (MQ=255)
                                                                                                cGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGa                                  >  2:20745/1‑134 (MQ=255)
                                                                                                cGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGa                                  >  7:65607/1‑134 (MQ=255)
                                                                                                      cccTAACCCTTGTAGAGGTTGTGCCACTGACAGTACCTACTGAGTATGCCTGCATCTGGCCGTAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGTGTGGGTGCGCTCTGCTCAAGCTGGAATAATc                            <  6:68344/134‑1 (MQ=255)
                                                                                                            cccTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCtt                       <  5:195803/133‑1 (MQ=255)
                                                                                                            cccTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCtt                       <  2:218466/133‑1 (MQ=255)
                                                                                                            cccTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCtt                       <  5:191964/133‑1 (MQ=255)
                                                                                                            cccTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGGGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTg                      <  5:103413/134‑1 (MQ=255)
                                                                                                              cTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGt                    >  4:172201/1‑134 (MQ=255)
                                                                                                                   agagGGTGTGCCACTGACAGTACCTACTGAGAATGc                                                                                                                 <  8:208984/36‑1 (MQ=255)
                                                                                                                   agagGGTGTGCCACTGACAGTACCTACTGAGAATGc                                                                                                                 >  7:208984/1‑36 (MQ=255)
                                                                                                                   agagGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGTAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAAt                >  2:189056/1‑133 (MQ=255)
                                                                                                                   agagGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAAt                >  5:170044/1‑133 (MQ=255)
                                                                                                                   agagGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAAt                >  6:129633/1‑133 (MQ=255)
                                                                                                                   agagGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAAt                >  6:58262/1‑133 (MQ=255)
                                                                                                                   agagGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACACACCCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAAt                >  5:29572/1‑133 (MQ=255)
                                                                                                                    gagGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCACCTTGGTCAATCTa             >  6:232120/1‑135 (MQ=255)
                                                                                                                       ggTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAgcg          >  4:231761/1‑135 (MQ=255)
                                                                                                                        gtgtGCCACTGAGAGTACCTACTGAGAATGACTGCATCTGGAAGGAGGCGGAGTAGCTGCAACAGAATCTCGAGACGCTCGCTGGGTGGGTGCGGTCTGCTCAAGCTCGTATAATCCCCTTGGTCAATCTAgcgc         <  2:125041/135‑1 (MQ=255)
                                                                                                                          gtgCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCg                                                                        >  3:202153/1‑70 (MQ=255)
                                                                                                                          gtgCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCg                                                                        >  3:198427/1‑70 (MQ=255)
                                                                                                                          gtgCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCg                                                                        <  4:198427/70‑1 (MQ=255)
                                                                                                                          gtgCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCg                                                                        <  4:202153/70‑1 (MQ=255)
                                                                                                                          gtgCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGccc       >  5:220822/1‑135 (MQ=255)
                                                                                                                          gtgCCACAGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCa                                         >  2:216753/1‑101 (MQ=255)
                                                                                                                          gtgCCACAGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCa                                         >  2:212838/1‑101 (MQ=255)
                                                                                                                          gtgCCACAGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCa                                         <  1:212838/101‑1 (MQ=255)
                                                                                                                          gtgCCACAGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCa                                         <  1:216753/101‑1 (MQ=255)
                                                                                                                            gCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGa     >  4:137454/1‑135 (MQ=255)
                                                                                                                            gCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGa     >  4:154619/1‑135 (MQ=255)
                                                                                                                            gCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGa     >  7:17179/1‑135 (MQ=255)
                                                                                                                            gCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGa     >  6:218336/1‑135 (MQ=255)
                                                                                                                              cACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAAc                                                                                 <  6:259584/57‑1 (MQ=255)
                                                                                                                              cACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAAc                                                                                 >  5:259584/1‑57 (MQ=255)
                                                                                                                              cACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGAcc   >  1:124056/1‑135 (MQ=255)
                                                                                                                              cACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGAcc   >  5:212113/1‑135 (MQ=255)
                                                                                                                              cACTGACAGTACCTACTGAGAATACCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGAcc   >  7:74626/1‑135 (MQ=255)
                                                                                                                               aCTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGCCCa  >  6:177378/1‑135 (MQ=255)
                                                                                                                               aCTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGACCa  <  6:18144/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                    
CCTCCCAGCTTCTGCGACTGGTTGACCAATTCGATGCCTGTCTGGCTGAGTTAGGTGGAGTTGTGGCTGTCCGAGATGAGCTGCTGAAGCTTCATGCGATGGCCCTAACCCTTGTAGAGGGTGTGCCACTGACAGTACCTACTGAGAATGCCTGCATCTGGACGGAGGCTGAGTCGCTGCAACAGAATCTCGAGACCCTCGCTGGGTGGGTGCGCTCTGCTCAAGCTGGAATAATCCCCTTGGTCAATCTAGCGCCCGACCA  >  KT2440/3362866‑3363127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑