| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,440,348 | T→G | 53.5% | A19A (GCT→GCG) | CJ019_03266 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,440,348 | 0 | T | G | 53.5% | ‑5.2 / 5.2 | 22 | A19A (GCT→GCG) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (5/4); new base G (7/5); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > KT2440/3440217‑3440473 | cAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGggc > 2:223100/1‑135 (MQ=255) gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGCCCCCCGGGCCGCCggggg > 8:13065/1‑134 (MQ=255) gcgggaggaTCGCAAGCCCCTCTACACGCGCGCGCTCGCGGTGCTGCAGGGCGCGCGTGGGCCCCCCGCCTGGGCGCCGGGGCGCTGCGGCGGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAg < 8:203903/132‑1 (MQ=255) tGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGCCGGCGCCCCGGCCGCCGGGGGGTGGCCGGc > 5:296879/1‑135 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGGCGGGGGGTGGCCGGCCCACGccga > 7:159671/1‑132 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCAACGccga > 8:21851/1‑132 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGACGGGGTGTGGGCGGCCACCGCCgat > 3:75274/1‑134 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCAGGCGGCGCCCCGGCCGCCTGGGTGTGGCAGGCCGACGccgaa > 3:157893/1‑131 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGGCGCCGGGGTGTGGGAGGCCCCCCCCGATAAGGCGCCgcgcgcggc > 2:188349/1‑131 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCGACGCCGATAAGGCGCCGCTCGcggg > 3:190579/1‑131 (MQ=255) ttgctgcAGTGAGGGGGGGGGCCCGGCGGCGGGGGGCGGTGGCGCGGCTGCGGCCCGCCCGCGCGGGCCGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATg < 5:102367/134‑1 (MQ=255) cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGGCTGGGGGTGGCGGGCCAACGCCTATAACGAGCCGCTCGCGGCTAATCCCGATGaggtcg > 5:140890/1‑130 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGGGTGGCAGGCCACCCCCGATAAGGCGCAGCTCGCGGAGAAGGCCGATGACTTCGGCcagcag > 7:12122/1‑135 (MQ=255) cTTGGGCGCCCCGGGGGGGCGGCGTGGCGCTCCGGGGGCCCGGCAGCGCTGGCCGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt < 7:13065/135‑1 (MQ=255) ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCCCCCCCGCGGGCGGCGCCCCGGCCGCCGGGGGGGGGGGGGCCCCCCCCCATAACGCGGCGCGCGCGGCTCAGGCCGCGGTCTTCGGGAACCacctggg > 2:112140/1‑129 (MQ=255) cGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCCCGGGCGGCGCCCCGGGCGCCGGGGGGTGGGCGGGCCACCCCCATAATGCGCAGCCCGCGGGTCCGGCCGCTTCCCTCGGCAAGCAGGTGGGGGGGAAg > 8:139557/1‑135 (MQ=255) cgcgggggCGCTGCTGCGGGCGGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 7:21851/129‑1 (MQ=255) cGCCGTGGCGCGGCTGCGGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 7:188349/135‑1 (MQ=255) cGCCGGGGGGGTGCGTCTGGCCGCGTGGGGTGGCGCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATAAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 5:87182/135‑1 (MQ=255) cgggCCCCCCGCGGTGGCGGCGCCCGGGCAGCCTGGGGGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg < 3:226119/133‑1 (MQ=255) cggcccgcccgcGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg < 3:187908/131‑1 (MQ=255) cgccCGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa < 3:115349/135‑1 (MQ=255) | CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > KT2440/3440217‑3440473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |