Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,667,362 T→C 8.9% H84R (CAT→CGT)  pyrR_1 ← Bifunctional protein PyrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,667,3620TC 8.9% 134.9 / 2.6 45H84R (CAT→CGT) pyrR_1Bifunctional protein PyrR
Reads supporting (aligned to +/- strand):  ref base T (20/21);  new base C (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTTCGGCGAGGTAGGCGCAGGTGCGTAGCAGAGAGTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATG  >  KT2440/3667229‑3667490
                                                                                                                                     |                                                                                                                                
cAGTTCGGCGAGGTAGGCGCAGGTGCGTAGCAGAGAGTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCTAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATg                                                                                                                                 <  8:65730/135‑1 (MQ=255)
  gTTCGGCGAGGTAGGCGCAGGTGCGTAGCAGAGAGTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCa                                                                                                                               <  6:251352/135‑1 (MQ=255)
  gTTCGGCGAGGTAGGCGCAGGTGCGTAGCAGAGAGTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCa                                                                                                                               <  5:277041/135‑1 (MQ=255)
                gcgcAGGTGCGTAGCAGAGAGTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGgtcg                                                                                                                 >  2:133642/1‑135 (MQ=255)
                   cAGGTGCGTAGCAGAGAGTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCt                                                                                                              <  4:112569/135‑1 (MQ=255)
                          gTAGCAGAGAGTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAgc                                                                                                       >  7:63228/1‑135 (MQ=255)
                          gTAGCAGAGAGTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAg                                                                                                        <  4:80028/134‑1 (MQ=255)
                              cAGAGAGTGCCCTTCGTAGAGCACGTCATTGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGAGCtt                                                                                                   >  3:41266/1‑135 (MQ=255)
                                    gTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCt                                                                                              <  5:175952/134‑1 (MQ=255)
                                        ccTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATc                                                                                         >  7:233779/1‑135 (MQ=255)
                                         cTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGAGCACGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCg                                                                                        >  4:262326/1‑135 (MQ=255)
                                             gTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCgg                                                                                       >  4:145128/1‑132 (MQ=255)
                                             gTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTGTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCGCAGGTCGTCTGCATAGCGCTTGACCTTGATCggg                                                                                      <  6:157677/133‑1 (MQ=255)
                                                   cACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATCCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCgg                                                                              <  3:19797/135‑1 (MQ=255)
                                                   cACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCGTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCgg                                                                              <  5:300445/135‑1 (MQ=255)
                                                   cACGTCAGCGACCACCAGCAAGGTGGTTCCGGCACGGTCGAGCTTGGCGAGTTCCGGGTGTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCgg                                                                              <  1:264901/135‑1 (MQ=255)
                                                    aCGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCgg                                                                              >  8:289681/1‑134 (MQ=255)
                                                     cGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGTGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCa                                                                            >  7:288630/1‑135 (MQ=255)
                                                      gTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATg                                                                                                                                 >  5:197554/1‑81 (MQ=255)
                                                      gTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATg                                                                                                                                 <  6:197554/81‑1 (MQ=255)
                                                         aTCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATgg                                                                        >  4:51195/1‑135 (MQ=255)
                                                             accaccAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCgca                                                                    <  8:128851/135‑1 (MQ=255)
                                                                accaGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCgcagca                                                                 >  7:13524/1‑135 (MQ=255)
                                                                  caGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCAcc                                                               <  4:284984/135‑1 (MQ=255)
                                                                     cAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGt                                                             >  3:249272/1‑134 (MQ=255)
                                                                     cAAGGTGGTTCCGGAAAGGTCGAGCTTGGAGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGAATGCAAGAGGCTCAGGTAGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGTCGCAGCACCGt                                                             >  3:50700/1‑134 (MQ=255)
                                                                           ggtTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATg                                                                                                                                 <  8:270182/60‑1 (MQ=255)
                                                                           ggtTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATg                                                                                                                                 >  7:270182/1‑60 (MQ=255)
                                                                             ttCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGGGAGTTGAGTATGCGCACGCAAGAGGCTCAGGTCGTCTTCATAGCGCGTGACCTTGATCGTGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCt                                                     <  3:262326/134‑1 (MQ=255)
                                                                               ccGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCtt                                                    >  4:73861/1‑133 (MQ=255)
                                                                               ccGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCtt                                                    >  3:207429/1‑133 (MQ=255)
                                                                               ccGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCACGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCgg                                                                              >  5:276659/1‑107 (MQ=255)
                                                                               ccGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCACGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCgg                                                                              <  6:276659/107‑1 (MQ=255)
                                                                                 ggCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAg                                                <  8:233809/135‑1 (MQ=255)
                                                                                 ggCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAg                                                <  6:36550/135‑1 (MQ=255)
                                                                                   cAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGt                                              <  8:13524/135‑1 (MQ=255)
                                                                                     aGGTCGAGCTTGGCGAGTTCCTGGGTTTCGGTGAGTTGAGTATGCTCATGCAAGAGGCTCAGGTCTTCTTAATAGAGCTTGACCTGGTTCGGGTATAGCGGCAATTGCGCAGCACCTTTCATTGCTTGCATGt                                              >  5:211748/1‑133 (MQ=255)
                                                                                         cGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGtt                                        <  7:291626/135‑1 (MQ=255)
                                                                                          gAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATATCGCTTGACCTTGATCGGGTTGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGtt                                        >  2:126842/1‑134 (MQ=255)
                                                                                          gAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGAACGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGtt                                        >  2:130957/1‑134 (MQ=255)
                                                                                                     gTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCt                                                                                                              <  7:57259/53‑1 (MQ=255)
                                                                                                     gTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCt                                                                                                              >  8:57259/1‑53 (MQ=255)
                                                                                                                       gttgagtCTGCGCATGCAAGAGGCTCAGGGCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGCGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCAcgcc          <  3:132326/135‑1 (MQ=255)
                                                                                                                       gttgagtATGCGCATGCAAGAGGCTCAGGGCGGCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCAcgcc          <  3:137280/135‑1 (MQ=255)
                                                                                                                               tGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCGTGATCGGGTAGAGCGGCAATGGCGCCGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCCGCGGCTCACCACCCCGCTGCATg  >  2:100533/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                
CAGTTCGGCGAGGTAGGCGCAGGTGCGTAGCAGAGAGTGCCCTTCGTAGAGCACGTCATCGACCACCAGCAAGGTGGTTCCGGCAAGGTCGAGCTTGGCGAGTTCCGGGTTTTCGGTGAGTTGAGTATGCGCATGCAAGAGGCTCAGGTCGTCTGCATAGCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATG  >  KT2440/3667229‑3667490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑