Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,761,329 A→C 30.5% E96A (GAG→GCG)  CJ019_03574 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,761,3290AC30.5% 36.1 / 3.5 44E96A (GAG→GCG) CJ019_03574hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (15/14);  new base C (12/2);  total (28/16)
Fisher's exact test for biased strand distribution p-value = 4.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCC  >  KT2440/3761201‑3761455
                                                                                                                                 |                                                                                                                              
cATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGGTCCCCCGCGCGCCgcg                                                                                                                          >  5:23943/1‑133 (MQ=255)
cATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCGTTTTCGGCCTGCTGGCCC‑AGCGCGCCGGCGGGCCgcg                                                                                                                          >  1:292100/1‑133 (MQ=255)
cATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCGTTTTCGGCCTGCTGACCC‑AGCTCGCCGGCGCGCCggg                                                                                                                          >  3:172121/1‑135 (MQ=255)
  tGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCGTCcgcgccggcgcgccggcc                                                                                                                         >  1:123868/1‑133 (MQ=255)
      ccAAGGAGAGCAAGACTTCAGCGATCCGACCCTGTCGGCCGGCGGTGACTGCGTGTTCGCCAGCCTGTTCAGCGCCACACTGCGGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGccc                                                                                                                    <  7:231534/135‑1 (MQ=255)
       cAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGCGCCGGGCAGcccc                                                                                                                   >  8:265201/1‑135 (MQ=255)
                 aaGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCgg                                                                                                                           >  3:198993/1‑117 (MQ=255)
                 aaGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCgg                                                                                                                           <  4:198993/117‑1 (MQ=255)
                 aaGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCGTACccaac                                                                                                          >  8:27807/1‑134 (MQ=255)
                  aGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCCGCCCCTTACcccacca                                                                                                        >  8:191545/1‑135 (MQ=255)
                           cGATCCGCCCCTGGCGGCCGGCGGTGCCTGCGTGATCGCCAGCCTGTGCCCCGCCACACTGCGGGCCGTCTTTTGCGGCCTGCTGACCC‑TGCGCGCCGGCGCGCCGGGCAGCCCCTTACCCAACCAACCTCCGcc                                                                                               <  7:27807/135‑1 (MQ=255)
                           cGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGcc                                                                                               <  3:174127/135‑1 (MQ=255)
                           cGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCGTTTTCGGCCTGCTGACCC‑AGCTCGCCGGCGCCCCGGGCCCCCCCGTACCCCACCCACCACcccc                                                                                               >  1:219492/1‑135 (MQ=255)
                             aTCCGACCCTGTGTGCCGGCGGTGACGGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCAc                                                                                             <  8:181676/135‑1 (MQ=255)
                                     cggTCTGCCGGCGGGGACGGCGTGAGCGCCAGCCTGTCCCGCGCCACACTGCGGGCCGTCTTTTGCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGccc                                                                                     <  7:273143/133‑1 (MQ=255)
                                     cTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGccc                                                                                     <  4:70643/135‑1 (MQ=255)
                                                cggTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCGTTTTAGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCCGCCCCGTACCCCCCCCCCCGCCGCCCCGCCGGCCCCAGCAGCGCTg                                                                          >  2:231714/1‑135 (MQ=255)
                                                cggTGACTGCGTGATCGACAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGGCGGGCAGCCCCCTACCCCACCACACGCCCCCCCGCCGGCCCCAACAgcgggg                                                                          >  4:270711/1‑132 (MQ=255)
                                                 ggggACGGCGCGATCGCCAGCCTGTGCCGCGCCCCACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCGCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTgg                                                                         <  7:285330/132‑1 (MQ=255)
                                                 ggTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTgg                                                                         <  7:265201/135‑1 (MQ=255)
                                                   tGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGCGCCGGGCAGTCCCTTACCCCACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGcc                                                                       >  4:168053/1‑135 (MQ=255)
                                                     aCTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCGCGCCGGCGCGCCGGGCCGCCCCGTACCCCCCCCACCTCCGCCCCGCCGGCCCCAGCCGCGCGGGcccc                                                                     >  3:118412/1‑133 (MQ=255)
                                                       tGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCGAGCCCGCGAGCCCGGCAGCCCCGTACCCAACCCCCCACCCCCCCCCCGGCCCAACCACCGCTGGCCACTg                                                                   >  3:62809/1‑135 (MQ=255)
                                                        gCGTGACCGCCCGCCTGGTCCGCGCCACACGGCGGGCCGTCTTTTGCGGCCTGCTGACCC‑GGCGCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTgg                                                                  <  1:145182/135‑1 (MQ=255)
                                                            gATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCCACCTCCGCCACCCCGGCCCAAGCCGCGCGGGCCACGGGTCaa                                                              >  7:85573/1‑135 (MQ=255)
                                                            gATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACAC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCAGCCCAAGCATCGCTGGCCACTGGTCaa                                                              >  8:133586/1‑135 (MQ=255)
                                                                  cAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGCCCC‑TGCTCGCCGGCGCGCCGGGCCGCCCCGTGCCCCCCCCCCCCCCGCCCCGCCGGCCCCACCCGCCCTGGGCACGGGGCAATCCCcg                                                        >  6:223608/1‑135 (MQ=255)
                                                                  cAGCCTGTGCCGCGCCACACTGCTGGCCGTCTTTTGCGGCCGGCTGACCC‑TGCGCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCcg                                                        <  8:186325/135‑1 (MQ=255)
                                                                      cTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGCGCCGGGCAGACCCTTACCCAACCAACCACCGCCCCGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGcc                                                    >  8:17181/1‑135 (MQ=255)
                                                                            aGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCGTACCCCACCAACCTCCGCCCCGCCTGCCCAAGCAGCGCGGGCCCCGGGTCAATCCCCGCGCCTCTccc                                              >  2:79710/1‑135 (MQ=255)
                                                                            aGCCCCACCCTGCGGGCGGTATTTTGCGGCCGGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTccc                                              <  3:103273/135‑1 (MQ=255)
                                                                                ccaccgtgctgGCCGTCTTTTTCGGCGGGCGGACCG‑GGCGCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgctt                                          <  7:200531/129‑1 (MQ=255)
                                                                                      ggctgGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCgcttcgcttc                                    <  4:182154/134‑1 (MQ=255)
                                                                                           gCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGCGCCGGGGAGCCCCTTACCCCACCACCCTCCCCCCCGCCGGCCCCAGCAGCGCGGGGCACTGGTCAATCCCCGCGCCTCACCCGCATCGCGTcgcgcg                               >  8:258101/1‑135 (MQ=255)
                                                                                           gCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGCGCCGGGCAGCCCCGTACCCAACCAACCTCCGCCACGCCTGCCCAAGCCGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTcgcg                               >  7:253355/1‑135 (MQ=255)
                                                                                           gCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGGCGCCCCGGACCCCACCACCCTCCGCCACGCCTGCCCAATCAGCGCTGGCCCCTGGTCCATCCCCGCGCCGCGCCCGCGTCGCGTcgcgcg                               >  4:280192/1‑135 (MQ=255)
                                                                                              gTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGCGCCGGGCCGCCCCTTACCCAACCCACCACCGCCCCGCCAGCCCCAGCAGCGCTGGCCCCTGGGCCATCCCCGCGCCTCTCCCGCGTCGCTTcgcgcgggg                            >  2:112834/1‑132 (MQ=255)
                                                                                                   tttCGGCCTGCTGACCC‑TGCTCGCCGGCGCCCCCGGCCGCCCCGTACCCCCCCCACCGCCCCCCCGCCGGCCCACCCAGCCCGGGCCACCGGTCAATCCCCCCGCCGCCCCCCCGTCGCCTCGCGCGGTCTGGCa                       >  4:106896/1‑135 (MQ=255)
                                                                                                   tttCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCCATCCCCGCCCCTCTACCGCTTCGCGTCTCGCGGTCTGACa                       >  1:243969/1‑135 (MQ=255)
                                                                                                                  cc‑TGCGCGCCGGCGCGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGt        <  1:231714/135‑1 (MQ=255)
                                                                                                                      gCTCGCCGGCGAGCCGGGCAGCCCCTTACCCCACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCGCGCGTGCTGCCATCGCCCCGTCCCCGGgcc     >  3:181545/1‑135 (MQ=255)
                                                                                                                         cGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCCCGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTGCGCTTGTCGCGGTCGGCCATCGCCACGTCCCCCGgccgcc  >  7:262984/1‑135 (MQ=255)
                                                                                                                          gCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTgccgcc  >  8:216762/1‑134 (MQ=255)
                                                                                                                           ccGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgcttcgctt                                     <  8:23565/98‑1 (MQ=255)
                                                                                                                           ccGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgcttcgctt                                     >  7:23565/1‑98 (MQ=255)
                                                                                                                                 |                                                                                                                              
CATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCC‑TGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCC  >  KT2440/3761201‑3761455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑