Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,945,826	C→A	8.4%	K123N (AAG→AAT)	CJ019_03731 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,945,826	0	C	A	8.4%	135.7 / 4.6	48	K123N (AAG→AAT)	CJ019_03731	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/22);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01

GTGCTGGCTGAGGTCGGCGTTGAAGCTGCCCAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGACACCACCGGGCATACCGTGGTGCAGCTGGTGT  >  KT2440/3945700‑3945959                                                                                                                               |                                                                                                                                      gTGCTGGCTGAGGTCGGCGTTGAAGCTGCCCAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGcgtc                                                                                                                               >  4:214278/1‑135 (MQ=255)       gctgAGGTCGGCGTTGAAGCTGCCCAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACg                                                                                                                         <  8:30118/135‑1 (MQ=255)        ctgAGGTCGGCGTTGAAGCTGCCCAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACg                                                                                                                         <  8:50899/134‑1 (MQ=255)        ctgAGGTCGGCGTTGAAGCTGCCCAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACg                                                                                                                         <  4:112511/134‑1 (MQ=255)               cGGCGTTGAAGCTGCCCAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCGCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGt                                                                                                                 <  5:169648/135‑1 (MQ=255)                               cAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGCCACTGATCGaca                                                                                                  >  4:50376/1‑134 (MQ=255)                               cAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGaca                                                                                                  >  2:165957/1‑134 (MQ=255)                               cAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACGGATCGaccc                                                                                                 >  7:4132/1‑133 (MQ=255)                               cAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCAGAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGacac                                                                                                 >  3:158574/1‑135 (MQ=255)                                    ccTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGCCACTGATCGACACCAGGc                                                                                            >  6:37336/1‑135 (MQ=255)                                          gggTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAAcct                                                                                      >  6:228031/1‑135 (MQ=255)                                                  gTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTTCAGGCGCTTGGCGTCGTCACGCAGAGGGTCGACACTGATCGACACCAGGCGAAACTCCTCGCCt                                                                              >  3:243331/1‑135 (MQ=255)                                                    gATGGCGGACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCGTGGCATAGTTTTGCAGGCGCGTGGCGGCGGCACGCTGAGGGTCGACACTGCGCGACACCAGGCGAACCTCCTCGCCTAc                                                                            <  5:17159/135‑1 (MQ=255)                                                          gTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGgcc                                                                      >  8:179138/1‑135 (MQ=255)                                                              ggggTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGccc                                                                  <  8:138516/135‑1 (MQ=255)                                                              ggggTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGccc                                                                  <  3:214278/135‑1 (MQ=255)                                                                ggatccGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCAAGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAg                                                                <  6:291133/131‑1 (MQ=255)                                                                     cGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAgctgct                                                           <  5:263672/135‑1 (MQ=255)                                                                     cGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAgctgct                                                           <  3:43509/135‑1 (MQ=255)                                                                             cagcTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCCGCGGCTGCTGGccc                                                   >  5:141107/1‑135 (MQ=255)                                                                             cagcTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGccc                                                   >  6:156472/1‑135 (MQ=255)                                                                               gcTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCAGCTGGACCtt                                                 >  2:185810/1‑135 (MQ=255)                                                                               gcTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGATTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCtt                                                 >  6:152123/1‑135 (MQ=255)                                                                               gcTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGATTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCtt                                                 >  6:147470/1‑135 (MQ=255)                                                                                         ggCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGAt                                        >  7:86708/1‑134 (MQ=255)                                                                                         ggCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATc                                       <  4:297356/135‑1 (MQ=255)                                                                                           cccTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGATTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATcga                                     <  5:147470/135‑1 (MQ=255)                                                                                           cccTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGATTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATcga                                     <  5:152123/135‑1 (MQ=255)                                                                                             cTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAAc                                                                                        <  1:203736/82‑1 (MQ=255)                                                                                             cTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAAc                                                                                        >  2:203736/1‑82 (MQ=255)                                                                                             cTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCACCTGCTGCTGGACCTTGCCCAAGATagacc                                   >  4:175090/1‑134 (MQ=255)                                                                                                  tGGAATGCCTTTGCATAGTTTTGCAGGCGCTTGGCGTCTTCAGGCGGAGGGTCTACACTGATCTAGACCAGGGGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGacac                               <  2:148045/134‑1 (MQ=255)                                                                                                  tGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGacac                               <  8:86708/134‑1 (MQ=255)                                                                                                  tGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGacac                               <  1:104585/134‑1 (MQ=255)                                                                                                  tGGAAGGCCTTGGCATTGTTTTGCAGGCGCTGGGCGTCGTCAGGCTGAGGGGCGACACTGAGCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGacac                               <  6:185344/134‑1 (MQ=255)                                                                                                     aaggCCTTGGCATAGTTTTGCAGGCGCTTGGCGGCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGAcacc                              <  8:201690/129‑1 (MQ=255)                                                                                                     aaTGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGGCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGAcaccac                            <  2:34264/134‑1 (MQ=255)                                                                                                         ccTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCGTCTGGACCATGCCCAAGATCGACGACACCACCggg                        >  4:253394/1‑134 (MQ=255)                                                                                                          cTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGCTCGACGCCACCCCCGGGcc                      >  8:67867/1‑134 (MQ=255)                                                                                                          cTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGACACCACCGGGc                       <  3:118429/134‑1 (MQ=255)                                                                                                             ggCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGACACCCCCGGGCATAc                   >  7:219195/1‑135 (MQ=255)                                                                                                                 tAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCGGCTGGGCCGTGCCCCTGCTCGCCGCCCCCACCGGGCAGACCggg               >  7:279234/1‑133 (MQ=255)                                                                                                                      ttGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCCGCAGCTGCAGGACCATGCCCCTGATCGCCGACACCACCCGGCATACCcgggtggc          >  3:157764/1‑133 (MQ=255)                                                                                                                      ttGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCACCTTCAGCTGGACCTTGCCCATGATCGACCACACCACCGGGCATACCGTGGTGCa          >  8:230661/1‑135 (MQ=255)                                                                                                                      ttGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCACCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGACACCACCGGGCATACCGTGGTGc           >  3:165281/1‑134 (MQ=255)                                                                                                                      ttGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCACCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGACACCACCGGGCATACCGTGGTGc           <  4:165281/134‑1 (MQ=255)                                                                                                                      ttGCAGGCGCTGGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCAACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGACACCACCGGGCATACCGTGGTGc           <  5:5469/134‑1 (MQ=255)                                                                                                                          aGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGACACCACCGGGCATACCGTGGTGCAGCTg      <  4:118776/135‑1 (MQ=255)                                                                                                                              gcTGGGCGGCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGACACCACCGGGCATACCGTGGTGCAGCTGgtgt  <  3:175090/135‑1 (MQ=255)                                                                                                                               |                                                                                                                                      GTGCTGGCTGAGGTCGGCGTTGAAGCTGCCCAGCCCCTTCAGGGTTTCGGTGATGGCGTACGGGGTACCGGTCAGCCAGCTCCAGCCCGGCCCTTTCTGGAATGCCTTGGCATAGTTTTGCAGGCGCTTGGCGTCGTCACGCTGAGGGTCGACACTGATCGACACCAGGCGAACCTCCTCGCCTACCCGGCCGCCCAGCTGCTGCTGGACCTTGCCCATGATCGACGACACCACCGGGCATACCGTGGTGCAGCTGGTGT  >  KT2440/3945700‑3945959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑