Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,957,060 A→T 11.1% Y197N (TAC→AAC)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,957,0600AT11.1% 144.3 / 6.5 61Y197N (TAC→AAC) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base A (26/28);  new base T (5/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCAGGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCA  >  KT2440/3956931‑3957191
                                                                                                                                 |                                                                                                                                   
gcagcaGGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCg                                                                                                                                <  5:215332/135‑1 (MQ=255)
gcagcaGGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCg                                                                                                                                <  3:198710/135‑1 (MQ=255)
    cagGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGATGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCGTCGGACAACCGGTa                                                                                                                            >  6:138630/1‑135 (MQ=255)
              cccatgcccaGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCGTCGTACCTCGGGTAAGCCCTgc                                                                                                                    >  6:95695/1‑133 (MQ=255)
                   gcccaGCTCGCGGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCgcaggca                                                                                                             <  7:121761/135‑1 (MQ=255)
                    cccaGCTCGCTGGCCACCATCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACCCCCAGTCGCCGTAGTACATAGGGTAAGACATGCgcaggcag                                                                                                            >  1:189546/1‑135 (MQ=255)
                    cccaGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCgcaggcag                                                                                                            >  2:78046/1‑135 (MQ=255)
                    cccaGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCgcaggcag                                                                                                            >  6:48956/1‑135 (MQ=255)
                      caGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCGTCGTACATCGGGTAAGCCATGCGCAGGCAGCg                                                                                                          >  7:163518/1‑135 (MQ=255)
                            gCTGGCCACCAGCATGGCGTCGGCGCCGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCACCCCCTTGCGGGAACACCCAGTCGCCGTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGcc                                                                                                    >  8:186489/1‑135 (MQ=255)
                            gCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCGTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGcc                                                                                                    >  3:93931/1‑135 (MQ=255)
                              tGGCCACCCGCATGGCGTCGGCGACGGTGGTAGAGCAACTGTCGTTTGGCGAGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGGCTCCTTCGTACATCGGGTAATCCATGCGCAGGCAGCGATGGCCGt                                                                                                  <  8:22473/135‑1 (MQ=255)
                              tGGACACCAGGATGGCGTCGGCGACGTTGGTAGAGAAACTGTAGTTTGGAGCGATCACGCTCTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGt                                                                                                  <  8:140474/135‑1 (MQ=255)
                                 ccaccaGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCCGCGATGGCCGTg                                                                                                 >  8:195599/1‑133 (MQ=255)
                                      aGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCt                                                                                          <  8:67426/135‑1 (MQ=255)
                                      aGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCGTCGGTCATCGGGTAAGCCCTGCGCCGGCAGCGATGGCCGTGCAAGTcg                                                                                          >  5:262229/1‑134 (MQ=255)
                                         aTGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCg                                                                                       <  1:78046/135‑1 (MQ=255)
                                         aTGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCg                                                                                       <  4:26693/135‑1 (MQ=255)
                                           ggCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                     <  5:115263/135‑1 (MQ=255)
                                           ggCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                     <  8:286474/135‑1 (MQ=255)
                                           ggCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                     <  5:95695/135‑1 (MQ=255)
                                           ggCGTCGGCGACGGTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                     <  7:27482/135‑1 (MQ=255)
                                              gtcgtcGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGgcg                                                                                  <  5:8182/135‑1 (MQ=255)
                                                 ggCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTc                                                                               <  7:140298/135‑1 (MQ=255)
                                                 ggCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGGCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTc                                                                               <  8:110915/135‑1 (MQ=255)
                                                  gCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTcc                                                                              >  7:49146/1‑135 (MQ=255)
                                                   cGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCg                                                                             <  5:65977/135‑1 (MQ=255)
                                                    gACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCCCGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCCTGCGCAGGCAGCGATGGCCGTGCAAATCTTCGGGGCGCTccc                                                                             >  6:58794/1‑133 (MQ=255)
                                                     aCGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGGTCATCGGGTAAGCCCTGCCCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGt                                                                           >  5:299181/1‑135 (MQ=255)
                                                      cGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTa                                                                          <  5:153807/135‑1 (MQ=255)
                                                         tGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTacac                                                                       <  8:4620/135‑1 (MQ=255)
                                                                   aCTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCAGGTACACCGTGGgtgt                                                              >  7:166118/1‑134 (MQ=255)
                                                                          tttGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTa                                                       <  6:103425/134‑1 (MQ=255)
                                                                              gcgcGATCACGCACTGGGCAACCCCTTGCGTGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCg                                                   <  5:145706/134‑1 (MQ=255)
                                                                               cgcgATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCgg                                                  <  6:232504/134‑1 (MQ=255)
                                                                                  gATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGc                                              >  7:235774/1‑135 (MQ=255)
                                                                                    tCACGCACTGGTCAACCGCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCcg                                            <  7:36048/135‑1 (MQ=255)
                                                                                                cAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGc                                <  5:48956/135‑1 (MQ=255)
                                                                                                cAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGc                                <  5:58794/135‑1 (MQ=255)
                                                                                                 aaCCCCTTGCGGGAACACCCAGTCGCGTTCGTTCATCGGGTAAGCCCTGCGCCGGCAGCGCTGGCCGTGCAAGTCTTCGGGGCGCGCCGGTACCCCGGGGGTGTGCAGGTAGCCGGGGGCCGCGCAGGGGATggg                               >  8:67592/1‑133 (MQ=255)
                                                                                                 aaCCCCTTGCGGGAACACCCAGTCGCGGTCGTACATCGGGTAAGCCCTGCGCCGGCAGCGCTGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACCCCGTGGGGGGCCAGGTAGCCGGGGGCCGCGCCGGGGGTGCt                               >  4:53491/1‑135 (MQ=255)
                                                                                                 aaCCCCTTGCGGGAACACCCAGTCGCCTTGGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCt                               >  8:32173/1‑135 (MQ=255)
                                                                                                 aaCCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTCAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCt                               >  1:16323/1‑135 (MQ=255)
                                                                                                 aaCCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGc                                >  8:240060/1‑134 (MQ=255)
                                                                                                 aaCCCCATGCGGGAACACCCAGTCGCCGTACTACAAACGGTAACACAAGCCCAGGCCGCGAATGCCCTGCAAATATTACGGGCGCTACCGTAAACCCTGGGTGTCCACGTATCCCGGGGGCCCGCCCGGGAAGCt                               >  6:138522/1‑135 (MQ=255)
                                                                                                     ccTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGaaa                           <  7:208439/135‑1 (MQ=255)
                                                                                                      cTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGaaa                           >  5:42899/1‑134 (MQ=255)
                                                                                                         gCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAg                                                                                             <  7:298069/65‑1 (MQ=255)
                                                                                                         gCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAg                                                                                             >  8:298069/1‑65 (MQ=255)
                                                                                                         gCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGGGTCCAGGTAGCCGGGGGCCGCGGAGGGGATGCTGAAAatat                       >  8:282123/1‑135 (MQ=255)
                                                                                                         gCGGGAACACCCAGTCGCCTTCGTAAATCGTGTACGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGGTGAAAatat                       >  8:27575/1‑135 (MQ=255)
                                                                                                          cGGGACCACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAata                        <  5:268564/133‑1 (MQ=255)
                                                                                                           gggAACACCCAGTCGCGGTCGTTCATCGGGTTAGCCATGCGCAGGCAGCGCTGGCCGTGCAAGTCGTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTTGCCGGGGGCCGCGCAGGGGATGCTGAAAATAttt                     >  5:68877/1‑134 (MQ=255)
                                                                                                           gggAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTAGGGGCGCTCCGGTACAACGTGGGTGTCCAGGTATCCCGGGGCCGCGCAGGGGATGCTGAAAAAAttt                     >  4:242135/1‑134 (MQ=255)
                                                                                                                cacCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCgg                                                  <  6:296823/101‑1 (MQ=255)
                                                                                                                cacCCAGTCGCCTTCCTACATACGGTAATCCAAGCGCAGGCACCGAAGGCCGTGCAAGTGTTCGGGGCGCTCCCGTACACCGTGGGTGTCCAGGTATCAGGGGGCCGCGCAGGGCATGCTGAAAATATAGACCAg                >  2:144792/1‑135 (MQ=255)
                                                                                                                cacCCAGTCGCCGTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCGTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCgg                                                  >  5:296823/1‑101 (MQ=255)
                                                                                                                    cAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGc            <  3:47828/135‑1 (MQ=255)
                                                                                                                    cAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGGGCAAGTCTTCGGGGCGCTCCGCAACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATAATGCCCAGCGGc            >  7:190153/1‑135 (MQ=255)
                                                                                                                        cgcggtCGTTCAACGGGTAAGCCAAGCGCAGGCAGCGATGGCCCGGGAAGTCTTCGGGGCGCTCCGGTTCACCGTGGGTGTCCAGGTAGCCCGGGGCCGCGCAGGGGTTGCTGAAAATTTTTACCAGCGGCACAg        >  4:47828/6‑135 (MQ=255)
                                                                                                                               gTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCa  <  7:186489/134‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                   
GCAGCAGGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCA  >  KT2440/3956931‑3957191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑