Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,957,251 G→A 45.5% P133L (CCG→CTG)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,957,2510GA45.5% 16.4 / 91.0 69P133L (CCG→CTG) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (21/16);  new base A (17/14);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

CGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAG  >  KT2440/3957123‑3957380
                                                                                                                                |                                                                                                                                 
cGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCgg                                                                                                                             <  5:222488/135‑1 (MQ=255)
cGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGGCATGCCCTTCGGCCAGCAGGTCgg                                                                                                                             <  7:32173/135‑1 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGt                                                                                                                          >  5:77758/1‑135 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGt                                                                                                                          >  6:246937/1‑135 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCgg                                                                                                                             >  5:226449/1‑132 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCgg                                                                                                                             <  6:226449/132‑1 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGt                                                                                                                          >  5:239597/1‑135 (MQ=255)
   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGCCCAGCAGGTCGGGGt                                                                                                                          >  7:207583/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCg                                                                                                                                   >  1:104626/1‑124 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCg                                                                                                                                   <  2:104626/124‑1 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTg                                                                                                                        >  2:27339/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                        >  6:157204/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                        >  3:233894/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                        >  3:221312/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                        >  1:99600/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCAGTCGGCCAGCAGCTCGGGGATg                                                                                                                        >  2:170735/1‑135 (MQ=255)
     gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGATGAAAATATTGCCCAGCGGCACAGCGCTCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCCGCGGGTCGGGGTTg                                                                                                                        >  8:128265/1‑135 (MQ=255)
       gtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACAGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTgcg                                                                                                                      >  7:231435/1‑135 (MQ=255)
           aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTg                                                                                                                   <  1:23463/134‑1 (MQ=255)
           aGGGAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAAGCCGGCAGCGCACCGTCCACGGTGATCACCACGGCATGCCCTTCGGCCAGCGGGTCGGGGTAGCGCTg                                                                                                                   <  6:25824/134‑1 (MQ=255)
                            gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                                 <  8:49146/135‑1 (MQ=255)
                              aGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGCTCCCCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATca                                                                                               >  4:15492/1‑135 (MQ=255)
                                       tGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGAGCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCg                                                                                      <  6:42899/135‑1 (MQ=255)
                                        gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                                     >  7:51232/1‑135 (MQ=255)
                                        gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                                     >  5:146981/1‑135 (MQ=255)
                                        gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                                     >  2:185814/1‑135 (MQ=255)
                                             tatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaaa                                                                                >  8:224583/1‑135 (MQ=255)
                                             tatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCCCTTCCGGGTaaa                                                                                >  2:175331/1‑135 (MQ=255)
                                                  cccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtg                                                                           >  4:168252/1‑135 (MQ=255)
                                                              gCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGCCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGcc                                                               <  8:207583/135‑1 (MQ=255)
                                                                  tCAGTTGCGAATCCGGCAGCTCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCTGGTCGGGGTTGCGCTGCGACAGCGGCAGCGCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccacca                                                           <  8:190153/135‑1 (MQ=255)
                                                                    aGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGc                                                         >  6:31696/1‑135 (MQ=255)
                                                                       tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                      <  7:67592/135‑1 (MQ=255)
                                                                       tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                      <  8:163518/135‑1 (MQ=255)
                                                                       tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                      <  2:99600/135‑1 (MQ=255)
                                                                         cGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCCCCAGCGGCa                                                     >  6:81968/1‑134 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                 <  6:108212/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                 <  5:138522/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                 <  6:262229/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                 <  7:282123/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                 <  4:221312/135‑1 (MQ=255)
                                                                            aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                 <  7:195599/135‑1 (MQ=255)
                                                                             tCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGGTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                 <  7:27575/134‑1 (MQ=255)
                                                                                   aGCGCCCCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATAACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCATCAACatc                                          >  5:15851/1‑133 (MQ=255)
                                                                                     cgcACCGTCCACGGTGATCACCACGTCATGCCCTTCGTCCAGCGGGTCGGGGTTGCGCTGCGACAGAGTCAGCTAGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                                        <  6:299181/135‑1 (MQ=255)
                                                                                               aCGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                                        <  5:208840/125‑1 (MQ=255)
                                                                                               aCGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                                        >  6:208840/1‑125 (MQ=255)
                                                                                                cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                             <  5:235327/135‑1 (MQ=255)
                                                                                                cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTACGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACTGCCCCATGCCGTGCg                             <  5:157204/135‑1 (MQ=255)
                                                                                                  gTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACgg                            >  2:88099/1‑134 (MQ=255)
                                                                                                    gATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCa                         >  4:240790/1‑135 (MQ=255)
                                                                                                        accacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCgg                                                       <  1:120667/101‑1 (MQ=255)
                                                                                                        accacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCgg                                                       >  2:120667/1‑101 (MQ=255)
                                                                                                         ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc                     >  6:284567/1‑134 (MQ=255)
                                                                                                         ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc                     >  3:161804/1‑134 (MQ=255)
                                                                                                           acGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCaaa                  >  2:268685/1‑135 (MQ=255)
                                                                                                              tCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc               <  4:233894/135‑1 (MQ=255)
                                                                                                                aTGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAAc                 >  5:82103/1‑131 (MQ=255)
                                                                                                                aTGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAAc                 >  3:237917/1‑131 (MQ=255)
                                                                                                                aTGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAc                 >  3:233620/1‑131 (MQ=255)
                                                                                                                     cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc        <  8:51232/135‑1 (MQ=255)
                                                                                                                     cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc        <  1:27339/135‑1 (MQ=255)
                                                                                                                     cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAGGCAGATGGc        <  2:297791/135‑1 (MQ=255)
                                                                                                                     cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc        <  3:168252/135‑1 (MQ=255)
                                                                                                                      ttCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGcc       <  8:117704/135‑1 (MQ=255)
                                                                                                                       tCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                 >  8:121918/1‑92 (MQ=255)
                                                                                                                       tCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                 <  7:121918/92‑1 (MQ=255)
                                                                                                                         ggCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTACCGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCATCAACAGACCCATGCCGTGCGGTCAGTGCAAACGCAGAAggacc      >  8:137045/1‑130 (MQ=255)
                                                                                                                          gCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGa   >  2:173837/1‑135 (MQ=255)
                                                                                                                          gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGa   >  6:214407/1‑135 (MQ=255)
                                                                                                                           ccAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCcgcgcg  >  3:124310/1‑133 (MQ=255)
                                                                                                                                |                                                                                                                                 
CGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAG  >  KT2440/3957123‑3957380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: