| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,957,351 | G→A | 47.5% | H100Y (CAC→TAC) | dmlR_13 ← | HTH‑type transcriptional regulator DmlR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,957,351 | 0 | G | A | 47.5% | 3.9 / 83.6 | 59 | H100Y (CAC→TAC) | dmlR_13 | HTH‑type transcriptional regulator DmlR |
| Reads supporting (aligned to +/- strand): ref base G (12/19); new base A (18/10); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-01 | |||||||||||
CGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGC > KT2440/3957219‑3957485 | cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg < 5:235327/135‑1 (MQ=255)cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTACGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACTGCCCCATGCCGTGCg < 5:157204/135‑1 (MQ=255) gTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACgg > 2:88099/1‑134 (MQ=255) gATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCa > 4:240790/1‑135 (MQ=255) ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc > 6:284567/1‑134 (MQ=255) ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc > 3:161804/1‑134 (MQ=255) acGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCaaa > 2:268685/1‑135 (MQ=255) tCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc < 4:233894/135‑1 (MQ=255) aTGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAAc > 5:82103/1‑131 (MQ=255) aTGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAAc > 3:237917/1‑131 (MQ=255) aTGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAc > 3:233620/1‑131 (MQ=255) cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc < 8:51232/135‑1 (MQ=255) cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc < 1:27339/135‑1 (MQ=255) cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAGGCAGATGGc < 2:297791/135‑1 (MQ=255) cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc < 3:168252/135‑1 (MQ=255) ttCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGcc < 8:117704/135‑1 (MQ=255) ggCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTACCGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCATCAACAGACCCATGCCGTGCGGTCAGTGCAAACGCAGAAggacc > 8:137045/1‑130 (MQ=255) gCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGa > 2:173837/1‑135 (MQ=255) gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGa > 6:214407/1‑135 (MQ=255) ccAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCcgcgcg > 3:124310/1‑133 (MQ=255) caggTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGt < 4:93931/133‑1 (MQ=255) ggcGGGGGTGCGCGGCGTAATCGGCATCTCGATAACCAAGTCCGGGTAAAGCCTGTTGTATCCGGCCAACAGCGGGATCAGCAACATCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAg < 8:231435/133‑1 (MQ=255) cAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCCGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGCGGCGGAAGGTGCGCCCCGCGCGCCTc > 6:196396/1‑135 (MQ=255) cAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTc > 8:296093/1‑135 (MQ=255) cAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTc > 4:240782/1‑135 (MQ=255) cAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCAc > 4:242073/1‑135 (MQ=255) gCTCGATCACCACTTCCTGGTAAAGCATGCTGTAGCCGGCCACCAGCGGCAGCAGCAACAGCCCCATGCCGGGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGGGCGCCCCTCGCGCCTCGCCCACgg < 1:95444/135‑1 (MQ=255) aTCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGcttct < 5:31696/135‑1 (MQ=255) caccacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCttcttc < 2:16323/135‑1 (MQ=255) accacTTCCGGGTAAAGCATGTTGTAGCAGGCCACCAGCGGCATCAGCAACAGACCCATGCCGTGCGGGCAGTGCAAAAGCAGATGGCCGCGACGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCGtcgtcg > 1:234220/1‑135 (MQ=255) cacTTCCGGGTTAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt < 2:142272/135‑1 (MQ=255) cacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt < 1:268685/135‑1 (MQ=255) ttCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGTGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCATCTTCGGTca > 4:114259/1‑134 (MQ=255) cGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTcagc < 4:161804/133‑1 (MQ=255) cGGGTAAAGCATGTTGAAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCTTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTcagc < 8:166118/133‑1 (MQ=255) ggTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAgcagca > 6:280721/1‑135 (MQ=255) gTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCAt < 3:35231/135‑1 (MQ=255) aTGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCCCGGCGTCTTCGGTCAGCAGCATGATCTGc > 6:193595/1‑135 (MQ=255) aTGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCCCGGCGTCTTCGGTCAGCAGCATGATCTGc > 1:168151/1‑135 (MQ=255) aTGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAAAGCAGATGGCCGCGAGGCGTAAGGTGCGCCACTCGCGCCTCGCCCACCCCGTCTTCGGTCAGCAGCATGATCTga > 5:102142/1‑134 (MQ=255) gttgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGAGGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcg < 5:196396/135‑1 (MQ=255) ctgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgc < 6:68877/134‑1 (MQ=255) tgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg < 6:254608/135‑1 (MQ=255) gtccccGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGGGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCATCGGTCACCAGCATGATCTGCCgcgc < 6:168222/131‑1 (MQ=255) gtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCgcgc > 8:280130/1‑135 (MQ=255) gtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCGTCTTCGGTCAGCAGCATGATCTGCCgcgc > 3:26373/1‑135 (MQ=255) tAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc < 8:300029/57‑1 (MQ=255) tAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc > 7:300029/1‑57 (MQ=255) tAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCa > 8:221946/1‑135 (MQ=255) gCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCg < 5:147319/105‑1 (MQ=255) gCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCg > 6:147319/1‑105 (MQ=255) gCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCCCGCt > 8:110125/1‑133 (MQ=255) caGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGaaa < 4:237917/135‑1 (MQ=255) ggCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGACCACGGCTTCTTAGGTCAGCAGCATGATCTGCCGCGCAGGCTCCAGAAACCGc < 7:137045/135‑1 (MQ=255) ggCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGc < 6:15851/135‑1 (MQ=255) gCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCg < 7:224583/135‑1 (MQ=255) gCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGc > 4:78950/1‑135 (MQ=255) cATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCAt < 5:284567/135‑1 (MQ=255) cGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCa > 3:241528/1‑135 (MQ=255) gCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGgc < 1:185814/135‑1 (MQ=255) | CGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAGGC > KT2440/3957219‑3957485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |