Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,970,221 C→T 7.8% D98N (GAC→AAC)  CJ019_03750 ← putative protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,970,2210CT 7.8% 148.7 / 2.9 51D98N (GAC→AAC) CJ019_03750putative protein
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base T (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGCGGGCGCTGTGCCGGTGCGAGATACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGAACGGGCCTTCGCCAAGC  >  KT2440/3970087‑3970351
                                                                                                                                      |                                                                                                                                  
gAAGCGGGCGCTGTGCCGGTGCGAGATACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTc                                                                                                                                    >  2:278052/1‑135 (MQ=255)
     gggCGCTGTGCCGGTGCGAGATACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGccg                                                                                                                               >  6:220297/1‑134 (MQ=255)
     gggCGCTGTGCCGGTGCGAGATACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCt                                                                                                                               >  2:147908/1‑135 (MQ=255)
          cTGTGCCGGTGCGAGATACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTTGGCCTggg                                                                                                                            >  3:56950/1‑133 (MQ=255)
          cTGTGCCGGTGCGAGATACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTggg                                                                                                                            >  6:223447/1‑133 (MQ=255)
              gCCGGTGCGAGATACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCa                                                                                                                      <  7:280121/135‑1 (MQ=255)
              gCCGGGGCGAGATACCGTCACGCTCGATGCGGTTGGTGCCGCTGGCAGCCACGGCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCa                                                                                                                      <  6:268357/135‑1 (MQ=255)
                          tACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGaaa                                                                                                          >  3:144842/1‑135 (MQ=255)
                          tACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGCGaaa                                                                                                          >  5:15173/1‑135 (MQ=255)
                          tACCGTCACGCGCGTTCCGGTTTGTGCCGCTGGCAGCCAGGTCAAGCAAGAGATCGACCACGGTTTCGGGCAGCTGGTTGACCTCATCGACTTACAGCTCACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGaaa                                                                                                          <  4:101047/135‑1 (MQ=255)
                              gTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCACGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTg                                                                                                      <  6:86821/135‑1 (MQ=255)
                                    cTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCtt                                                                                                <  7:97544/135‑1 (MQ=255)
                                    cTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCtt                                                                                                <  5:223447/135‑1 (MQ=255)
                                    cTCGAGGCGGTGTGTGCCGCTGGCAGCCACGTCAAGCAACAGCTCGACCACGGTGTCGGGCAGCAGGTGGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCtt                                                                                                <  8:141464/135‑1 (MQ=255)
                                     tCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTtg                                                                                               <  2:244009/135‑1 (MQ=255)
                                     tCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTtg                                                                                               >  2:17222/1‑135 (MQ=255)
                                     tCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTtg                                                                                               <  5:139065/135‑1 (MQ=255)
                                       gATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTTGGCCTGGGCCCGCACGCCCGGGGAAAACTGCGCCTtgcc                                                                                             >  6:35888/1‑135 (MQ=255)
                                       gATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTtgcc                                                                                             >  6:11218/1‑135 (MQ=255)
                                          gCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGAACGCCCGGGGAAACCTGCGCCTtgccctg                                                                                          >  7:193005/1‑135 (MQ=255)
                                            ggTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTtgccctgcc                                                                                        <  7:47148/135‑1 (MQ=255)
                                                tgtgCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAg                                                                                    >  1:216976/1‑135 (MQ=255)
                                                     cGCTGTCAGCCACGTCAAGCAACAGATCGACCATGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCgg                                                                               <  2:6025/135‑1 (MQ=255)
                                                      gCTGTCAGCCACGTCAAGCAACACATCTACCATGGGGTCGGGCAGAAGGTTGACCTCATGGAAATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGAGCCTTGCCCTGCCCCAGCGCGGc                                                                              <  6:15173/135‑1 (MQ=255)
                                                      gCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGc                                                                              >  3:7043/1‑135 (MQ=255)
                                                          gCAGCCACGTCAACCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTAGCCCTGCCCCAGCGCGGCAt                                                                            >  3:235770/1‑133 (MQ=255)
                                                              ccACGTCAAGCAACAGATCGACCATGGTGTCGGGCAGCAGGTGGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGt                                                                      <  4:255530/135‑1 (MQ=255)
                                                              ccACGTCAAGCAACAGATCGACCAAGGTGGCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGt                                                                      <  5:253052/135‑1 (MQ=255)
                                                                         aaCAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCg                                                           <  3:145809/135‑1 (MQ=255)
                                                                              aTCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAg                                                      >  8:161779/1‑135 (MQ=255)
                                                                                 gACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGCCCAGCCg                                                   >  4:175496/1‑135 (MQ=255)
                                                                                   ccAATGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGCCCAGCACGCCCGGGGAAAAATGCGCCTTGCCCTGCCACAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGct                                                 >  6:87683/1‑135 (MQ=255)
                                                                                             gggCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGgc                                       <  3:180299/135‑1 (MQ=255)
                                                                                             gggCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGCGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGCTGCCGACCAGCCGCGCTTCACTGgc                                       >  1:126811/1‑135 (MQ=255)
                                                                                                    agGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAAt                                <  6:120988/135‑1 (MQ=255)
                                                                                                    agGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGGGCCGACCAGCCGCTCTTCACTGGCGCCCAAt                                <  4:171056/135‑1 (MQ=255)
                                                                                                    agGGTGACCTCATCGACATACCGCACACCGCCGGCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCATGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAAt                                <  4:69888/135‑1 (MQ=255)
                                                                                                      gTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCTGCATCCAGGTCGAGGGTGCCGACCAg                                                      <  5:206855/111‑1 (MQ=255)
                                                                                                      gTTGACCTCATCGACATACAGCACACCGCAGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAg                                                      >  6:206855/1‑111 (MQ=255)
                                                                                                           ccTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAg                          <  7:161779/134‑1 (MQ=255)
                                                                                                             tCATCGACATACAGCACACCGCCGTCGGCCTCGGCCAGCACGCCCGGGGAAAACTGCGCATTGCCCTGCCCCAGCGCCTCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAAAGGCAAGGTg                       >  2:251615/1‑135 (MQ=255)
                                                                                                                tCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACg                    <  4:7043/135‑1 (MQ=255)
                                                                                                                  gACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGaa                  >  7:207974/1‑135 (MQ=255)
                                                                                                                    cATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGAACg                <  5:11218/135‑1 (MQ=255)
                                                                                                                    cATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGAACg                <  1:39757/135‑1 (MQ=255)
                                                                                                                    cATACAGCACACCGCCGGTGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGTCGCCCAATGGCAAGGTGACGAACg                <  5:35888/135‑1 (MQ=255)
                                                                                                                              acCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCCAGGTGACGAACGGGCCGTCGcc      >  5:37761/1‑135 (MQ=255)
                                                                                                                              acCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGAACGGGCCTTCGc       >  6:65422/1‑134 (MQ=255)
                                                                                                                                 gccgTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGAACGGGCCTTCGCCAAg   >  3:58359/1‑135 (MQ=255)
                                                                                                                                 gccgTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGAACGGGCCTTCGCCAAg   >  3:155481/1‑135 (MQ=255)
                                                                                                                                 gccgTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGAACGGGCCTTCGCCAAg   >  2:68560/1‑135 (MQ=255)
                                                                                                                                  ccgTTGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGAACGGGCCTTCGCCAAGc  <  4:56950/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GAAGCGGGCGCTGTGCCGGTGCGAGATACCGTCACGCTCGATGCGGTTTGTGCCGCTGGCAGCCACGTCAAGCAACAGATCGACCAAGGTGTCGGGCAGCAGGTTGACCTCATCGACATACAGCACACCGCCGTCGGCCTGGGCCAGCACGCCCGGGGAAAACTGCGCCTTGCCCTGCCCCAGCGCGGCATCCAGGTCGAGGGTGCCGACCAGCCGCTCTTCACTGGCGCCCAATGGCAAGGTGACGAACGGGCCTTCGCCAAGC  >  KT2440/3970087‑3970351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑