Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,986,622	T→G	35.0%	D31A (GAT→GCT)	CJ019_03763 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,986,622	0	T	G	35.0%	28.1 / 2.4	43	D31A (GAT→GCT)	CJ019_03763	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (13/13);  new base G (6/8);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGCCATCAGCACCTGAGCCAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTGGGTTAAGGGT  >  KT2440/3986496‑3986752                                                                                                                               |                                                                                                                                   gACGCCATCAGCACCTGAGCCAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCGCCGGcgcg                                                                                                                            >  1:47521/1‑135 (MQ=255)   ggCCATCAGCCCCTGTCCCACCAAGTGTTCTGGGTACAAGCCATTGGGGGCCAGCTTCGGGTCCTGTTGCTCAGGGGAGAACACCGGGTCCTTGGCTCCGAGGGGGGCCGCGGACGGCGCCGGATCCGGCGCgg                                                                                                                           <  2:268308/133‑1 (MQ=255)      cATCAGCACCTGAGCCAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCGCGGGcgc                                                                                                                       >  2:147394/1‑133 (MQ=255)      cATCAGCACCTGAGCCAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCGCGGGccc                                                                                                                       >  2:104082/1‑133 (MQ=255)      cATCAGCACCTGAGCCAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCCGCGACGGCGCGGGccc                                                                                                                       >  2:184974/1‑133 (MQ=255)       aTCAGCCCCTGAGCCAGCAACTGATCCGGGTACACGGCAAGGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACg                                                                                                                      <  2:235727/135‑1 (MQ=255)            cACCTGAGCCAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATACGGCGCGGGCCCGTCTGc                                                                                                                 >  6:230457/1‑135 (MQ=255)                    ccAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCCCGTCTGCCGCCTGCa                                                                                                         >  4:1120/1‑135 (MQ=255)                        cAACTGAGCAGGGGACACGGCAAGGGGGGCCAGCAGCTGGGCCAGTGGCGCCGGGGAGAACCCCGGGGCCTGGGCTCCGAGCGGGGCCGCGGAAGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCAccag                                                                                                     <  8:46440/135‑1 (MQ=255)                                      aCAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGCAGGCGGCCGATACGGCGCGGGGACGTCTGCGGGCTGCCCCAGCGCAGGGGCCgcgc                                                                                       >  2:46244/1‑135 (MQ=255)                                      aCAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGGCGGCGCCGGCGCGGGCCCGTCTGCGGCCGGCCCCAGGCCCGGGGgcgcgc                                                                                       >  3:84741/1‑135 (MQ=255)                                                gggggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCGCGGGGACGTCTGCGGCCTGCCCCAGGCCAGGGGCCGCGCGCAGGCcaa                                                                              >  6:297225/1‑134 (MQ=255)                                                gggggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCGCGGGCCCCGCTGCGGCCTGCCCCCCGCCCGGGGCCGCGCGCCGCCAAAg                                                                             >  8:163539/1‑135 (MQ=255)                                                gggggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACCTCTGCGGCCTGCACCACGCCAGGGGGATCGCGCAgcgaaa                                                                              >  2:91414/1‑129 (MQ=255)                                                gggggCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGAGGCGGCGCGGGCGCGTCGTCGGCCGGCCCCAGCCCCGGGGGGGCGCGCCGGCcaa                                                                              >  3:18230/1‑134 (MQ=255)                                                      cAGCATCTGGTCCAGTGGCTCCTGGGAGAACACCGGGGCCTTGGCTACGAGCGTGGCAGCGGACGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGc                                                                       <  2:208800/135‑1 (MQ=255)                                                        gCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCCCGGGCACGTCGGCGGCCGGCCCCAGGCCCCGGGCCGCGCGCCGGCAAAGCCGTGCCg                                                                     >  3:23080/1‑135 (MQ=255)                                                         cATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCTCCGGCGCGGGCACGGCTGCGGCCTGCCCCAGGCCACGGGCAGCGCTCAGCCAAACCAGGGccca                                                                    >  4:29416/1‑133 (MQ=255)                                                                        cTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGGTCCGGCGCGGGCACGTCGGCGGCCGGCACCAGGCCCGGGGGCGCCCGCAGGCCAAGCAGTGGCGCCAGGTAAGCAAACaa                                                     >  6:283144/1‑135 (MQ=255)                                                                        cTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCCCCCGCGCCGGCGCCGGGCCCTCGGCGGCCGGCGCCCCGCCCGGGGGCCCGCCCCGGGCAAGCACTGCGCCCCCGAAAGCAAACa                                                      >  4:270419/1‑134 (MQ=255)                                                                              ggAGAACACCGGGTCCTTGGCTACGAGCGGGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCAt                                               <  6:220065/135‑1 (MQ=255)                                                                                agacccccGGGGCCTGGGCTACGAGCGGGGCAGCGGACGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATgc                                             <  3:42356/129‑1 (MQ=255)                                                                                agaCCACCGGGTCCTTGGCTCCGCGCGTGGCAGCGGACGGCGCCGGAGCCGCCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATgc                                             <  5:251638/135‑1 (MQ=255)                                                                                agaACCCCGGGTCGTTGGCTACGCGCGTGGCTGCGGATGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATgc                                             <  8:93174/135‑1 (MQ=255)                                                                                agaACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGGACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATgc                                             <  8:284768/135‑1 (MQ=255)                                                                                agaACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATgc                                             <  7:29130/135‑1 (MQ=255)                                                                                    cacGGGGTCCTTGGCGACGCGCGGGGCTGCGGACGGCCGGGGCTCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCAt                                         <  5:93233/135‑1 (MQ=255)                                                                                     acCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGCGCCGGCGCGGGCCCGGCTGCGGGCGGCCCCCCGCCCCGGGCCGCGCGCCCGCCAACCAGGGCCGCCCGGCAAGCCAACAATGGCATgcgccac                                        >  4:151581/1‑132 (MQ=255)                                                                                         ggTCCTTGGCGACGCGGGGGGCCGCGGACGGCGCCGGACCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCtt                                    <  7:91494/135‑1 (MQ=255)                                                                                            ccTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCCGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGCAAGCAAAAAAAGGCATGCGCATCGCTTATc                                 >  8:240972/1‑135 (MQ=255)                                                                                             gtgggCTCCGCGCGTGGCAGCGGCCGGCGCCGGCGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATcc                                <  1:147394/132‑1 (MQ=255)                                                                                             cTTGGCTAGGAGCGTGGCAGCGTAGGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATcc                                <  2:273526/135‑1 (MQ=255)                                                                                               tGGCTACGAGGGTGGGAGCGGAAGGCGCGGGATCGGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATcccc                              <  4:292789/135‑1 (MQ=255)                                                                                                       cgCGGGGCCGCGGACGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTc                      <  7:141940/134‑1 (MQ=255)                                                                                                            ggCCGCGGCAGGCGCGGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGAcc                 <  1:12462/135‑1 (MQ=255)                                                                                                            ggCAGCGTACGGCGCGGGATCCGGCGCGGGCACGTGTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGAcc                 <  4:18230/135‑1 (MQ=255)                                                                                                            ggCAGCGGCCGGCGCCGGACCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGAcc                 <  1:104082/135‑1 (MQ=255)                                                                                                            ggCAGCGGCAGGCGCCGGATCCGGCGCGGGCACGGCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGAcc                 <  6:103/135‑1 (MQ=255)                                                                                                                 cGGAAGGCGCCGGTCCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTg            <  2:212966/135‑1 (MQ=255)                                                                                                                  gtaaGGCGCCGGAGCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTgg           <  5:140853/133‑1 (MQ=255)                                                                                                                  ggACGGCGCCGGATCGGGCGCGGGCACGTCTGCGGCCTGCACCCGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTgg           <  6:183367/135‑1 (MQ=255)                                                                                                                    aaGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGCCAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGCCCGCGTggg          >  8:230377/1‑134 (MQ=255)                                                                                                                           cGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTGGGTTAAGGGt  <  2:89946/135‑1 (MQ=255)                                                                                                                               |                                                                                                                                   GACGCCATCAGCACCTGAGCCAGCAACTGATCAGGGTACAAGGCAATGGGGGCCAGCATCTGGTCCAGTTGCTCCTGGGAGAACACCGGGTCCTTGGCTACGAGCGTGGCAGCGGAAGGCGCCGGATCCGGCGCGGGCACGTCTGCGGCCTGCACCAGGCCAGGGGCAGCGCTCAGGCAAAGCAGTGCCGACAGGAAAGCAAACAATGGCATGCGCATCGCTTATCCCCATGAGGTCAGACCGCGTGGGTTAAGGGT  >  KT2440/3986496‑3986752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑