Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,989,732 T→C 6.1% L31P (CTG→CCG)  CJ019_03768 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,989,7320TC 6.1% 215.4 / 4.2 66L31P (CTG→CCG) CJ019_03768hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/35);  new base C (2/2);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

AAAGCCCGGCATGGAGGCCTTTAACCAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGTCCGCGATTACATCCATGA  >  KT2440/3989601‑3989864
                                                                                                                                   |                                                                                                                                    
aaaGCCCGGCATGGAGGCCTTTAACCAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCg                                                                                                                                   <  5:131469/135‑1 (MQ=255)
  aGCCCGGCATGGAGGCCTTTAACCAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCgg                                                                                                                                  >  6:72499/1‑134 (MQ=255)
  aGCCCGGCATGGAGGCCTTTAACCAAGAAGGAAAACCAAGGAGCAAATTTGATTTTCAGCTGGCCTACACCATCACACCGCACAACCCTGCGTACGATGAACCCGATGCGGCACAGGCACGTCTGCCTCTGCgg                                                                                                                                  >  6:172869/1‑134 (MQ=255)
     cctgCATGGAGGCCTTTAACCAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGaaa                                                                                                                              <  4:125159/132‑1 (MQ=255)
       ggCATGGAGGCCTTTAACCAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAg                                                                                                                            <  3:261058/135‑1 (MQ=255)
          aTGGAGGCCTTTAACCAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTc                                                                                                                         >  7:124675/1‑135 (MQ=255)
            ggAGGCCTTTAACCAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCgg                                                                                                                       >  1:109112/1‑135 (MQ=255)
                 ccTTTAACCAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTgg                                                                                                                  >  2:111085/1‑135 (MQ=255)
                        ccAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGt                                                                                                           >  6:105698/1‑135 (MQ=255)
                         cAAGCACGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTc                                                                                                          <  3:41038/135‑1 (MQ=255)
                         cAAGAAGGAACACCAAGGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACACCCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTc                                                                                                          <  7:237403/135‑1 (MQ=255)
                         cAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAACCCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGTCCTGGACACCGTc                                                                                                          <  1:185351/135‑1 (MQ=255)
                         cAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTc                                                                                                          <  7:266162/135‑1 (MQ=255)
                         cAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTc                                                                                                          <  7:60640/135‑1 (MQ=255)
                                        aTGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAAcc                                                                                           <  7:284276/135‑1 (MQ=255)
                                           aGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCAc                                                                                         >  7:3592/1‑134 (MQ=255)
                                            gCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCTGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACgc                                                                                       <  8:27612/135‑1 (MQ=255)
                                             cAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGaaa                                                                                                                              >  4:256/1‑95 (MQ=255)
                                             cAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGaaa                                                                                                                              <  3:256/95‑1 (MQ=255)
                                                  ttGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTTCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTg                                                                                 <  7:30715/135‑1 (MQ=255)
                                                          cAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATc                                                                         >  5:224570/1‑135 (MQ=255)
                                                           aGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATc                                                                         <  5:22104/134‑1 (MQ=255)
                                                                 ccTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACt                                                                    <  3:4459/133‑1 (MQ=255)
                                                                   tACACCATCAAACCGCACAACCCTGCGTACGATGAACCCGATGCGGCACAGGCACGGCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAAt                                                                <  3:163393/135‑1 (MQ=255)
                                                                       ccATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGca                                                            <  6:52276/135‑1 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTc                                                                                                                         <  2:54952/68‑1 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTc                                                                                                                         >  1:54952/1‑68 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAAcc                                                                                           >  2:173505/1‑98 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAAcc                                                                                           <  1:173505/98‑1 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCAccc                                                      >  6:97586/1‑135 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCAccc                                                      >  4:87689/1‑135 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCAccc                                                      >  4:53908/1‑135 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCAccc                                                      >  4:126585/1‑135 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCAccc                                                      >  2:141264/1‑135 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCAccc                                                      >  3:197220/1‑135 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCCGCGGGAAAAGCTc                                                                                                                         <  8:269214/68‑1 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCCGCGGGAAAAGCTc                                                                                                                         <  8:264975/68‑1 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCCGCGGGAAAAGCTc                                                                                                                         >  7:269214/1‑68 (MQ=255)
                                                                             aaCCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCCGCGGGAAAAGCTc                                                                                                                         >  7:264975/1‑68 (MQ=255)
                                                                               ccGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTc                                                    <  8:17378/135‑1 (MQ=255)
                                                                                     aaCCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGAc                                              >  2:249039/1‑135 (MQ=255)
                                                                                     aaCCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGAc                                              >  4:154092/1‑135 (MQ=255)
                                                                                          tGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCaa                                         <  5:105698/135‑1 (MQ=255)
                                                                                             gTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCg                                      >  7:253461/1‑135 (MQ=255)
                                                                                               aCGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGa                                                                           <  4:84109/96‑1 (MQ=255)
                                                                                               aCGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGa                                                                           >  3:84109/1‑96 (MQ=255)
                                                                                                 gATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGa                                   <  3:87689/134‑1 (MQ=255)
                                                                                                    gAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGc                                >  4:76101/1‑134 (MQ=255)
                                                                                                       aCCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGaa                            <  8:253461/135‑1 (MQ=255)
                                                                                                             gCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTg                      <  1:249039/135‑1 (MQ=255)
                                                                                                              cGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTTGACACCGTCGAGCAGATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCCAGCGGGAGGCTGAAAAGCTgg                     <  3:224878/135‑1 (MQ=255)
                                                                                                               ggCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTgg                     <  3:289372/134‑1 (MQ=255)
                                                                                                               ggCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGt                    <  1:141264/135‑1 (MQ=255)
                                                                                                               ggCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGt                    <  3:53908/135‑1 (MQ=255)
                                                                                                                     ggCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGTCCGCGa              <  6:126601/135‑1 (MQ=255)
                                                                                                                     ggCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGTCCGCGa              <  2:20772/135‑1 (MQ=255)
                                                                                                                         cGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGTCCGCGATTAc          >  5:110384/1‑135 (MQ=255)
                                                                                                                              gCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGa                                                                           <  1:81506/65‑1 (MQ=255)
                                                                                                                              gCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGa                                                                           >  2:81506/1‑65 (MQ=255)
                                                                                                                              gCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACt                                                                    >  1:163888/1‑72 (MQ=255)
                                                                                                                              gCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACt                                                                    <  2:163888/72‑1 (MQ=255)
                                                                                                                              gCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGTCCGCGATTACATCCa     >  2:140912/1‑135 (MQ=255)
                                                                                                                              gCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGTCCGCGATTACATCCa     >  6:29795/1‑135 (MQ=255)
                                                                                                                                 tCTGCGGGAAAAGCTCGGCCTGGACACCGTCGATCACATTGAAACCACGCTGCTTGGCATGATCAGACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGTCCGCGATTACATCCATGa  <  5:227549/135‑1 (MQ=255)
                                                                                                                                 tCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCTCCACCCTCGCTGACCGCAAGCGGGAGGTTGAAAAGCTGGTCCGCGATTACATCCATGa  <  5:28351/135‑1 (MQ=255)
                                                                                                                                 tCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGTCCGCGATTACATCCATGa  <  2:109112/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
AAAGCCCGGCATGGAGGCCTTTAACCAAGAAGGAAAACCAATGAGCAAATTTGATTTTCAGCTGGCCTACACCATCAAACCGCACAACCCTGCGTACGATGAAACCGATGCGGCACAGGCACGTCTGCATCTGCGGGAAAAGCTCGGCCTGGACACCGTCGAGCACATTGAAACCACGCTGCTTGGCATGATCACACTCAATGGCACCACCCTCGCTGACCGCAAGCGGGAGGCTGAAAAGCTGGTCCGCGATTACATCCATGA  >  KT2440/3989601‑3989864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑