Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,092,063 A→C 21.5% V91G (GTC→GGC)  CJ019_03853 ← 2‑haloacrylate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,092,0630AC21.5% 90.0 / 3.4 72V91G (GTC→GGC) CJ019_038532‑haloacrylate reductase
Reads supporting (aligned to +/- strand):  ref base A (22/32);  new base C (8/10);  total (30/42)
Fisher's exact test for biased strand distribution p-value = 7.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCTTGCGCTG  >  KT2440/4091934‑4092196
                                                                                                                                  |                                                                                                                                     
gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCACCTGCGGCGGCAGCGGCACCAGCCGCTCGGCCGGGGCCCGCCGGCCCC‑GGGCGTAGGCCCCGCCCGGc                                                                                                                                  >  5:164175/1‑135 (MQ=255)
gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCCGCGGAACCAGCCGCTCGGCCGGGCACAGCCCGCCCC‑TGGCATAGGCACCGCCCCGc                                                                                                                                  >  1:133406/1‑135 (MQ=255)
gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGc                                                                                                                                  >  4:130659/1‑135 (MQ=255)
gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGAGACGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGc                                                                                                                                  >  2:123662/1‑135 (MQ=255)
   gtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGCACAGCCGGCCAC‑TGGCATAGGCACCGACCGGcc                                                                                                                                 >  6:119768/1‑133 (MQ=255)
       tCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATcg                                                                                                                           >  1:91388/1‑135 (MQ=255)
        cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGGACACCCCGCCAC‑TGGCATAGGCACCGACCCGCCCCAAcgc                                                                                                                          >  5:41336/1‑135 (MQ=255)
        cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCCCCGCCCGGCCCCAAcgc                                                                                                                          >  5:89940/1‑135 (MQ=255)
        cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATcgc                                                                                                                          >  4:46251/1‑135 (MQ=255)
         aaCATATATTGCGCGGTTTTTCTCTTGAACAGCACCGCGGCGGCGGCGTGGTCCTCCAACTGCGGCGGCAGCGTAACCAGCCGCTCGGCGGGTAACAGCGGGCCAC‑TGGAATAGGCACCGACGGGCCCAATcgc                                                                                                                          <  6:90324/134‑1 (MQ=255)
             gATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAg                                                                                                                     >  1:260350/1‑135 (MQ=255)
                 ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGAc                                                                                                                 >  1:17251/1‑135 (MQ=255)
                 ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGAc                                                                                                                 >  7:227756/1‑135 (MQ=255)
                   gcgcCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGcccc                                                                                                               >  6:226220/1‑135 (MQ=255)
                     gcCGTGATTCCCTTGACCAGCACCGCTGCGGCGGCCTCGGCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTACGCGACCCgg                                                                                                             <  6:112011/135‑1 (MQ=255)
                     gcCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGCACCGCCCGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCgg                                                                                                             >  7:112893/1‑135 (MQ=255)
                     gcCGTGATACCCTTGAACCGCCCCGCGGCGGCGGCCTCGCCCCCCAACGGCGGCGGCCGCGGACCCAGCCGCGCGGCCGGGAACAGCCGGCCAC‑TGGCTTAGCCACCGACCGCCCCCAGCGCGTAAGCGACCCgg                                                                                                             <  6:231559/135‑1 (MQ=255)
                      ccGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGt                                                                                                            >  1:234733/1‑135 (MQ=255)
                      ccGGGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCGCGGCCGGTAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGt                                                                                                            <  1:202499/135‑1 (MQ=255)
                       ggTGATTCCCTGGAACCGCCCCGCGGCGGCGGCCGCGCCCACCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGCCACCGACCGGCCCCATCGCGTAAGCGACCCGGTc                                                                                                           <  5:25580/134‑1 (MQ=255)
                       cGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCCGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTc                                                                                                           <  5:116962/135‑1 (MQ=255)
                        gTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCCGGAACAGCCCGCCCC‑AGGCATAGGCACCGCCCGGCCCCAACGCGTAAGCGAccccggcg                                                                                                          >  1:300148/1‑130 (MQ=255)
                         tGATTCCCTTGAACAGCACCGCTGCGGCGGCCACGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGGCCC‑AGGCATAGGCACCGACCGGCCCCCTCGCGGAAGCGCCCCGGgcgc                                                                                                         >  7:257363/1‑135 (MQ=255)
                            ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCCCCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCa                                                                                                      >  6:16205/1‑135 (MQ=255)
                            ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGCCCCGCCCCATCGCGTAAGCGACCCGGTCGCCCa                                                                                                      >  6:20091/1‑135 (MQ=255)
                               cctgcaaCCGCCCCGCGGCGGCGGCCGCGGCCCCCACGTGCGGCGGCAGCGGAACCAGCCGCGCGGCCGGTAACAGCCGGCCAC‑TGGCATCGGCACCGCCCGGCCCCATCGCGAAAGCGACCCGGTCGCCCACCt                                                                                                   <  4:46443/130‑1 (MQ=255)
                                 ttGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGCACAGCCCGCCAC‑AGGCATAGGCACCGACCGGCCCCAACGCGTAAGCGCCCCCGTCGCCCCCCAGc                                                                                                 >  5:279569/1‑135 (MQ=255)
                                 ttGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGc                                                                                                 <  4:271889/135‑1 (MQ=255)
                                 tagAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGc                                                                                                 <  7:89964/133‑1 (MQ=255)
                                      cAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCCGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGtt                                                                                            <  4:33344/135‑1 (MQ=255)
                                      cAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGCAACCAGCCGCGCGGCCGGGAACAGCCGGCCAC‑TGGCATAGCCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGtt                                                                                            <  6:58227/135‑1 (MQ=255)
                                            cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGCACAGCCGGCCAC‑TGGCCTAGGCACCCCCCGGCCCCAACGCGGAAGCCACCCCGTCGCCCCCCAGCCCGGGGTGCAc                                                                                      >  1:289140/1‑135 (MQ=255)
                                            cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGCCCCGGTCGCCCCCCTGCACGGTGTGCAc                                                                                      >  8:296428/1‑135 (MQ=255)
                                            cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑AGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCAGCACCTTGTGccc                                                                                      >  7:159235/1‑133 (MQ=255)
                                             gctcgggcggcCTCGTCCGCCACCGGCGGCGGCAGCGTAACCAGCCGCTCGGCGGGGAACCCCCGGCAAC‑GGGCATAGGCACCCCCCGGCCCCACCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCAcc                                                                                     <  4:279108/130‑1 (MQ=255)
                                                 cggcggCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGGCAC‑TGGCATAGGCCCCGACCGGCCCCCTCGCCTAAGCGACCCGGTCGCCCACCTGCCCGTTGGGGACCCCag                                                                                 >  5:297542/1‑134 (MQ=255)
                                                 cggcggCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCCTCGCGTAAGCGACCCGGTCGCCCACCTGCCCGTTGTGCAccccct                                                                                 >  2:206200/1‑133 (MQ=255)
                                                 cggcggCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGCCCCGGTCGCCCCCCGGCCCGTTGTGCAccccct                                                                                 >  5:21321/1‑133 (MQ=255)
                                                   ggggcCGCGCCCCCCAACTGCGGCGGCCGGGGAACCAGCCGCGCGGCCGGGAACAGCCGCCCAG‑GGCCATAGGCACCCACCGGCCCCATCGGGAAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                               <  1:113652/133‑1 (MQ=255)
                                                   gcggcCTCGTCCTCCACCTGCGGCGGCAGCGGAACCAGCCGCGCGGCCGGGACCAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                               <  7:296428/135‑1 (MQ=255)
                                                   gcggcCTCGTCCGCCACCGGCGGCGGCAGCGGAACCCGCCGCGCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                               <  1:188486/135‑1 (MQ=255)
                                                   gcgccCTCGGCCGCCATCGGGGGCGCCTGCGTAACCCGCCGCGCGGCGGGCAAC‑GCCGGCCACGGGGAATTGCCACCCCCGGGCCCCAGCGCGACAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                               <  6:182321/135‑1 (MQ=255)
                                                   ccggccgcggccgCCACCTGCGGCGGCCGCGCACCCCGCCGCGCGGCCGGTAACAGCCGGGCAC‑GGGCATCGCCACCCCCCGCCCCCAGCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                               <  5:25073/134‑1 (MQ=255)
                                                    cggcCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                               >  8:280757/1‑134 (MQ=255)
                                                    cggcCTCGTCCGCCAACTGCGGCGCCTGCGGAACACGCCGCGCGGCCGGCAACAGCCGGCACG‑TGGCATAGGCACCGACCGGCCCCATCGCTTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGc                                                                              <  8:286375/135‑1 (MQ=255)
                                                    cggcCGCGCCCGCCACCTGCGCCGGCTGGGGAACCCGCGGCTGGGCCGGTAACTGCCGGCCAC‑TGGCATTGGCACCGCCCGGACCCAGCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGc                                                                              <  6:4885/135‑1 (MQ=255)
                                                         tCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGCACAGCCCGCCAC‑TGGCATCCGCACCGCCCGGCCCCCTAGCGTAAGCGACCCCCTCCCCCCCCTGCCCGGAGTGCACCCCCACCCCAATg                                                                         >  7:226983/1‑135 (MQ=255)
                                                              cgccACCTGCGCGGGAAGCGGAACCCGCCGCGCGGCCGGTAACAGCCGCCAAC‑TGGCATAGGCACCGACCGCCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCga                                                                    <  2:105053/133‑1 (MQ=255)
                                                                     tGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTg                                                             <  7:96095/135‑1 (MQ=255)
                                                                      gcggcgGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCCCCCCATCGCCAATGGCCGAGACCCTg                                                             >  7:254972/1‑134 (MQ=255)
                                                                        ggcggcAGCGGAACCCGCCGCGCGGCCGGCAACCGCCGGCCAC‑TGGCATAGCCACCGCCCGCCCCCAACGCGAAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                          <  4:280023/135‑1 (MQ=255)
                                                                        gccggcAGCGCAACCAGCCGCGCGGCCGGCACCAGCCGGCAAC‑TGGCATCGCCCCCGACCGCCCCCAGCGCTTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                          <  6:28702/133‑1 (MQ=255)
                                                                              aGCGCAACCCCCCGCTCGGCCGGGAACAGCCGGCCAG‑TGCCATAGGCACCGACCGGCCCCAGCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCt                                                    <  6:248309/135‑1 (MQ=255)
                                                                                   aaCCCGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAg                                               <  2:67187/135‑1 (MQ=255)
                                                                                     ccAGCGGCTCGGCGGGGACCAGCCGGCCAC‑TGGCATAGGCACCCACGGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTc                                             <  7:4395/135‑1 (MQ=255)
                                                                                     ccAGCCGCTGGGCCGGGAACAGCCGCCCAC‑GGGAATCGGCACCGACCGGCCCCACCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTc                                             <  2:274080/135‑1 (MQ=255)
                                                                                     ccAGCCGCTCGGCCGGGAACCGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCTTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATAGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTc                                             <  6:73580/135‑1 (MQ=255)
                                                                                           gCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGc                                       <  7:80344/135‑1 (MQ=255)
                                                                                           gCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGc                                       <  8:227756/135‑1 (MQ=255)
                                                                                                gcgggtaaCACCCGGCCAG‑TGGCATCGCCACCCCCCGGCCCCTGGGCGACCGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGaa                                  <  2:289140/129‑1 (MQ=255)
                                                                                                gcggggAACACCCGGCAAC‑TGGCATAGGCACCGACCGGCCCCATCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGTCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGaa                                  <  6:89940/132‑1 (MQ=255)
                                                                                                gCCGGTAACAGCCGGCCAC‑TCCCTTAGGCACCGACCGGCCCCATCGCGAAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGaa                                  <  1:208411/135‑1 (MQ=255)
                                                                                                     gAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCaa                             <  4:89310/135‑1 (MQ=255)
                                                                                                          gcgggCCAT‑TGGAATCGGCACGCACCGCCCCCCGCGCTAAAGCGACCCGGTCGCCGACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCa                        <  6:193585/132‑1 (MQ=255)
                                                                                                              gCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAgcgc                    >  6:95139/1‑135 (MQ=255)
                                                                                                                cAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCgctg                  <  6:43251/135‑1 (MQ=255)
                                                                                                                cAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCgctg                  <  2:240933/135‑1 (MQ=255)
                                                                                                                cAC‑GGGCATAGGCACCGCCCGGCCCCCTCGCGGACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCgctg                  <  2:234733/135‑1 (MQ=255)
                                                                                                                      gattAGGCCCCCCCCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTccc             <  6:197414/132‑1 (MQ=255)
                                                                                                                      gCATCGGCACCGACCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTccc             <  1:193097/135‑1 (MQ=255)
                                                                                                                       cATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGGCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTccc             <  8:650/134‑1 (MQ=255)
                                                                                                                            gCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCtt        <  2:17251/134‑1 (MQ=255)
                                                                                                                               ccgaccgGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCTTgcgc    <  4:24542/135‑1 (MQ=255)
                                                                                                                                 caccgGCCCCATCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCTTGCGCTg  <  6:41336/134‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
GTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCAC‑TGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCTTGCGCTG  >  KT2440/4091934‑4092196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑