Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,096,610 G→A 7.5% A94V (GCC→GTC)  CJ019_03858 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,096,6100GA 7.5% 162.3 / 2.5 53A94V (GCC→GTC) CJ019_03858hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/22);  new base A (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

GAGAGGTAACCTTGACCTGGCGGAACGCCTCGCCGCCCGGGTTGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCACCGTGACTTGTCGGGTG  >  KT2440/4096485‑4096741
                                                                                                                             |                                                                                                                                   
gcgcggTAACCGTGACCGGGCGGACCGCCCCGCCGCCCGGGTTGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGgcgc                                                                                                                            <  4:21179/131‑1 (MQ=255)
                cTGGCGGAACGCCTCGCCGCCCGGGTTGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCCCATCCGGCTTGAGCACATAGGCCACGGCGCCTGTCCCGGAGCTGTc                                                                                                            >  5:265223/1‑135 (MQ=255)
                     ggAACGCCTCGCCGCCCGGGTTGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACtt                                                                                                       >  8:35661/1‑135 (MQ=255)
                      gAACGCCTCGCCGCCCGGGTTGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGCCTTg                                                                                                      >  4:119448/1‑135 (MQ=255)
                        aCGCCTCGCCGCCCGGGTTGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGt                                                                                                    <  6:52455/135‑1 (MQ=255)
                         ggCCTCGCCGGCCGGTGTGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGGCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCAGGTAGATGTCGAGTTGGTc                                                                                                   <  1:207519/134‑1 (MQ=255)
                         ccccccgccgcCCGGGGTGGGATTTTGCGCGGTTGCACGCTGGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTc                                                                                                   <  5:185009/130‑1 (MQ=255)
                            cTCGCCGCCCGGGTTGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCCCTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCAcc                                                                                                >  6:66087/1‑135 (MQ=255)
                                          tGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTgg                                                                                  <  7:27865/135‑1 (MQ=255)
                                           gggATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACgg                                                                                                                               <  6:276706/89‑1 (MQ=255)
                                           gggATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACgg                                                                                                                               >  5:276706/1‑89 (MQ=255)
                                              aTTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAg                                                                              >  5:53586/1‑135 (MQ=255)
                                                    cTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCtt                                                                         <  6:53586/134‑1 (MQ=255)
                                                     tCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGCCTTTAAGGTTGGGCAGCGCTTTg                                                                       >  6:75549/1‑135 (MQ=255)
                                                                    gTACCGGACTCTTCCGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGt                                                        <  8:294000/135‑1 (MQ=255)
                                                                           cctctTCAGTCGCATTCGGCGCCCCCCCCACTTCCGGATTGAGCACATACGCCACGGCGCGGGTCACGTAGATGTCGACTTGGGCACCGACCTTAAGGTTGGGGAGCGCTTTGGCCTTTTCGGTCAGTTGGAACg                                                 <  4:55190/134‑1 (MQ=255)
                                                                           aCTCTTCTGACGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACg                                                 <  2:300562/135‑1 (MQ=255)
                                                                           aCTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGCTTGGCAGCGCTATGGCCTTTTCGGTCACTTGGAACg                                                 <  6:155430/135‑1 (MQ=255)
                                                                           aCTCTTCAGTCGCTTGAGGCGCCCCCCCGACTTCCTGATTGAGAACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACg                                                 <  6:293770/135‑1 (MQ=255)
                                                                              ctTCAGTCGCTTTCGGCTCACCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTca                                              <  6:64859/135‑1 (MQ=255)
                                                                              ctTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCtttt                                                                >  6:57096/1‑117 (MQ=255)
                                                                              ctTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCtttt                                                                <  5:57096/117‑1 (MQ=255)
                                                                              ctTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTc                                               >  1:156128/1‑134 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGCGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTGTAAGGTTGGGCCGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATc                                          >  5:43861/1‑135 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCAGGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATc                                          >  8:220051/1‑135 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTTGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGtt                                                       <  1:34661/122‑1 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGtt                                                       >  2:34661/1‑122 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATc                                          >  8:43286/1‑135 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATc                                          >  3:120802/1‑135 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGAcct                                                                                                                                  <  6:245095/47‑2 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGAcct                                                                                                                                  >  5:245095/1‑46 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGAcct                                                                                                                                  >  3:132827/1‑46 (MQ=255)
                                                                                  aGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGAcct                                                                                                                                  <  4:132827/47‑2 (MQ=255)
                                                                                  aGTCGCTTTCGGCGACCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTTGTCACCGACTTTAAGGATGGGCAGCGCTTTGGACTTTTAGGTCAGTTGGAACGTCACAt                                           >  5:64859/1‑134 (MQ=255)
                                                                                    tCGCTTTCGGCGCCCCCCCCACTTCCGGATTGCGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCct                                        >  4:236008/1‑135 (MQ=255)
                                                                                      gCTTTCTGCTCCACCCCAACTTCCGGATTGTGCACATAGGCCACGTCGAGTGTCACGTAGACGTCGACTTGGTCCCGTAATGTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCcttc                                      <  7:169907/135‑1 (MQ=255)
                                                                                       cTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTc                                                               <  8:297734/109‑1 (MQ=255)
                                                                                       cTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTc                                                               >  7:297734/1‑109 (MQ=255)
                                                                                          tCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTtcttct                                  >  4:194531/1‑135 (MQ=255)
                                                                                          tCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCCTTTCGGTCAGTTGGAACGTCACATCCTtcttct                                  >  5:58370/1‑135 (MQ=255)
                                                                                            ggCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCCCGGCGCGGGTCACGTAGATGTCGACTTGGTCACCGACGTTAAGGTTGGGCAGCGCTTTGGCCGTTTCGGTCAGTTGGAACGTCACCTCCTTCGTCTCg                                >  5:231172/1‑135 (MQ=255)
                                                                                                    ccccACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGcc                        <  5:109508/135‑1 (MQ=255)
                                                                                                              ggATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCACCgag              >  7:33733/1‑133 (MQ=255)
                                                                                                              ggATTGAGCACATAGGCCACGGCGCGTGTCACGGAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCACCGt               <  8:130586/134‑1 (MQ=255)
                                                                                                                aTTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCCCCGTgcc            >  2:131560/1‑133 (MQ=255)
                                                                                                                aTTGAGCACATAGGCCACCGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGTTTGGGCAGCGCTATGGCCTTTTCGGTCAGTTGGAACGTCACAACCTTCTTCTCGTCAGGGCCTTTCCCCGTGAc            >  5:190475/1‑135 (MQ=255)
                                                                                                                 ttGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCACCGTGACt           >  7:143777/1‑135 (MQ=255)
                                                                                                                       acaTAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTgg                                                                                  <  7:195265/58‑1 (MQ=255)
                                                                                                                       acaTAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTgg                                                                                  >  8:195265/1‑58 (MQ=255)
                                                                                                                       acaTAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCACCGTGACTTGTCgg     >  3:183920/1‑135 (MQ=255)
                                                                                                                       acaTAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCACCGTGACTTGTCgg     >  6:31814/1‑135 (MQ=255)
                                                                                                                         aTAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCACCGTGACTTGTCGGGt   <  8:226633/135‑1 (MQ=255)
                                                                                                                          tAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCCTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCACCGTGACTTGTCGGGTg  <  6:58370/135‑1 (MQ=255)
                                                                                                                            ggCCACGGCGCGTGTCACGTAGATGTCGACTTGGTc                                                                                                   >  3:240517/1‑36 (MQ=255)
                                                                                                                            ggCCACGGCGCGTGTCACGTAGATGTCGACTTGGTc                                                                                                   <  4:240517/36‑1 (MQ=255)
                                                                                                                             gCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACg                                                 >  7:185921/1‑85 (MQ=255)
                                                                                                                             gCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACg                                                 <  8:185921/85‑1 (MQ=255)
                                                                                                                             |                                                                                                                                   
GAGAGGTAACCTTGACCTGGCGGAACGCCTCGCCGCCCGGGTTGGGATTTTGCTCGGTTGCACGGATGGTACCGGACTCTTCAGTCGCTTTCGGCGCCCCCCCCACTTCCGGATTGAGCACATAGGCCACGGCGCGTGTCACGTAGATGTCGACTTGGTCACCGACTTTAAGGTTGGGCAGCGCTTTGGCCTTTTCGGTCAGTTGGAACGTCACATCCTTCTTCTCGTCAGGGCCTTTCACCGTGACTTGTCGGGTG  >  KT2440/4096485‑4096741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑