Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,135,592 A→C 17.0% N27T (AAC→ACC)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,135,5920AC17.0% 107.8 / 4.1 65N27T (AAC→ACC) tgnC(Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (33/19);  new base C (3/10);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAA  >  KT2440/4135458‑4135724
                                                                                                                                      |                                                                                                                                    
tgGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGCCGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACCGCCTCaa                                                                                                                                      >  8:124534/1‑135 (MQ=255)
tgGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCaa                                                                                                                                      >  1:85858/1‑135 (MQ=255)
tgGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCaa                                                                                                                                      <  8:244797/135‑1 (MQ=255)
  gACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAAcc                                                                                                                                    >  7:49282/1‑135 (MQ=255)
     ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg                                                                                                                                 <  8:108232/135‑1 (MQ=255)
     ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg                                                                                                                                 <  4:159514/135‑1 (MQ=255)
     ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg                                                                                                                                 <  8:160494/135‑1 (MQ=255)
     ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg                                                                                                                                 <  3:285843/135‑1 (MQ=255)
     ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg                                                                                                                                 <  3:267672/135‑1 (MQ=255)
       ccTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGcc                                                                                                                               >  5:115783/1‑135 (MQ=255)
          cACCCCCGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGCCGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGCCAGCCTCAACCCGGCCAcc                                                                                                                            >  7:163946/1‑135 (MQ=255)
          cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCAcc                                                                                                                            >  3:84917/1‑135 (MQ=255)
          cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc                                                                                                                            >  1:123386/1‑135 (MQ=255)
          cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc                                                                                                                            >  7:292662/1‑135 (MQ=255)
          cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc                                                                                                                            >  4:68498/1‑135 (MQ=255)
          cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc                                                                                                                            >  8:130963/1‑135 (MQ=255)
          cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCATCGACAGCCTCAACCCGGCCAcc                                                                                                                            >  3:30123/1‑135 (MQ=255)
            cccAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc                                                                                                                            <  2:20109/133‑1 (MQ=255)
                 gCCCGCACCCTCACCGAATCCGCCAAGGGGATGGTGGCATGCCCCTCGTGCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAgg                                                                                                                     <  1:75863/135‑1 (MQ=255)
                    cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGccg                                                                                                                  >  1:4614/1‑134 (MQ=255)
                    agCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATTGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCCACCCGGCCACCCCCCCGCCCt                                                                                                                  >  4:208855/2‑135 (MQ=255)
                          cTCCCCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGgcgc                                                                                                            >  1:113673/1‑135 (MQ=255)
                                gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCCGCCTGGGCGCCACGGc                                                                                                      >  3:202923/1‑135 (MQ=255)
                                gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGACGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCGGCCTGGGCGCAACTGc                                                                                                      >  8:118891/1‑135 (MQ=255)
                                      gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCCCCCAGGCCTGGGCGCACCTGCCCGAcc                                                                                                >  2:16626/1‑134 (MQ=255)
                                      gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGCCCTGGGCGCAACTGCCCCACg                                                                                                >  1:56871/1‑135 (MQ=255)
                                            gggATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCAGCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCGTGGGCGCAACTGCCCGACGCCGATg                                                                                          <  1:54872/135‑1 (MQ=255)
                                             gttttgttgCATGACCCTCGTTCGTTTCCATATGGGCATCGCCGGCCACGGGCGCGATGCCCAGAGCGGCAACACCTGCGACAGCCTCAACCCGGCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa                                                                                         <  4:132115/132‑1 (MQ=255)
                                             ggtttgttgCTTGACCCTCGTTGGTTGCCATATGTGCATCGACGGCCAATGGCGCGAGGCCCAGAGCGCCAAGACCTACGACCGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa                                                                                         <  2:134656/132‑1 (MQ=255)
                                             ggATTGTTGCATGACCCTCGTTCTTTTCCTGATGGGCATCGCCGGCCAATGGCGCGATGCCCAGAGCGCCAATACCTTCGACAGCCTCAACCCGGCCACCGCCCTGGCCTGGGCGCAACTGCCCGACGCCGATGa                                                                                         <  4:94575/135‑1 (MQ=255)
                                             ggATTGTTGCATGACCCGGGTTCGTTTCCAGTTGTGCTTCGACGGCCAAGGGCGCGAGGCCCAGAGCGGCAAGACGTTCGACAGCCTCACCCGGGCCACGGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa                                                                                         <  4:84917/135‑1 (MQ=255)
                                                   ttgCATGACCCTCGTGCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCg                                                                                    <  8:250846/134‑1 (MQ=255)
                                                    tgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGAt                                                                                  >  6:240007/1‑135 (MQ=255)
                                                    tgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCACGGCGCCACAGCCCCACGCCGCAGACGCCGaa                                                                                  >  1:194854/1‑134 (MQ=255)
                                                                  tcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACAGCCCGCCGCCCAAGAAGCCGATGTGGGGCTGGCCGt                                                                    >  6:104943/1‑135 (MQ=255)
                                                                  tcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCAACTGCCCGACGCCGCAGAAGCCGATGTGGAGCTGGCCg                                                                     >  8:132314/1‑134 (MQ=255)
                                                                   cgttCCCAGCTGCGCCGCGCCGCCCAAGGGCGCGAGGCCCCGCGCGCCAACCCCTGCGACCCCCTCACCCCGGCCACCCCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGtt                                                                   <  7:165472/135‑1 (MQ=255)
                                                                         cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGGGCCACTGCCCGCCGCCGCAGACGCCGCGGGTGAGCTGGCCGTGCCGgcc                                                             >  5:35874/1‑135 (MQ=255)
                                                                         cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCCGGGCGCCACAGCCCCACGCCCCGGCACCCGATGTGGCGCGTGCCGGTCACgcc                                                             >  2:16213/1‑135 (MQ=255)
                                                                          aGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCCCCCCGGCGCCACAGCCCGCCCCCCATGACGCCCAGGTGGAGCGGGCCGATCAGtccg                                                            >  7:180290/1‑135 (MQ=255)
                                                                                  aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCACTGCCCGCCGCCGCTGAAGCCGCTGTGGCGCGGGCCGGTCAGGCCGccccccgg                                                    >  6:287829/1‑130 (MQ=255)
                                                                                    cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAAccca                                                                                                                                  >  4:183429/1‑54 (MQ=255)
                                                                                    cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAAccca                                                                                                                                  <  3:183429/55‑2 (MQ=255)
                                                                                       cGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGACTCAACCCGGCCACCGCCCAGGCCTGGGCGCGACTTCCCGCCGCAGATGAAGCCGATGTGGCGCTGGCCGTTCAGGCCGCCCCGCGTGccgt                                               >  7:297011/1‑133 (MQ=255)
                                                                                       cGGCCAAGGGCGCGAGGCCCAGCGCGGCTAGCCCTGCGCCAGCCGCACCCCGGCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCtt                                               <  7:32580/135‑1 (MQ=255)
                                                                                            aagggCGCGATGCCCAGCGCGCCAAGACCTTCGACAGCCTCACCCCGGCCCCCCCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa                                          <  8:162995/132‑1 (MQ=255)
                                                                                            aaggccgcgAGGCCCAGCCCGCCACCACCTTCGACCCCCGCACCCCGCCCCCCGCCCCGGCCGGGGCGGAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa                                          <  2:60265/130‑1 (MQ=255)
                                                                                            aaTGGCGCGATGCCCAGAGCGGCAACACCTGCGACCCCCCCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa                                          <  1:178985/135‑1 (MQ=255)
                                                                                            aaTGGCGCGAGGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa                                          <  8:292662/135‑1 (MQ=255)
                                                                                                    gATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCGCTGTGGCGCTGGCCGTTCAGGCCGCCCCCCGTGCCGTCGCCCCCAAGGCg                                  >  2:281720/1‑135 (MQ=255)
                                                                                                      tGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGCCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg                                <  8:3838/135‑1 (MQ=255)
                                                                                                      ggCCCAGAGCGGCAAGCCCTGCGACAGCCTCACCCCGGCCACCCCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg                                <  2:169040/134‑1 (MQ=255)
                                                                                                         ccAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCCCAGCCCGCCGCCGCGGAAGCCGCTGTGGGGCTGGCCGGTCAGGCCGCCCCGCGGGCCGTCGACAGCCACGCGTGgcg                             >  1:264018/1‑135 (MQ=255)
                                                                                                         ccAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGATGCAGCCGATGTGGCGCTGGCCGTTCAGGCCGCCCCCCGTGCCTTCGCCCGCAAGGCGGGgcg                             >  6:235756/1‑135 (MQ=255)
                                                                                                            gcgcgcCAAGCCCTTCGACCCCCCCACCCCGCCCCCCCCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag                          <  8:44759/133‑1 (MQ=255)
                                                                                                            gagCGGCACGCCCTGCCACCGCCTCAACCCCGCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag                          <  4:128892/135‑1 (MQ=255)
                                                                                                            gagCGGCAAGACCTTCGACAGCCGCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag                          <  2:42986/135‑1 (MQ=255)
                                                                                                               cGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCAGGGCGCAACAGCCCGCCGCCGCAGAAGCCGCTGTGGAGCTGGCCGTTCAGGCCGCCCCGCGGGGCTTGGCCCGCAAGGCGTGGCGCAGCAt                       >  2:217936/1‑135 (MQ=255)
                                                                                                                ggCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACAGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGCCGTTCAGGCCGCCCCGCGTGCCGTCGACAGCAAGGCGTGGCGCAGCATCa                     >  1:49947/1‑135 (MQ=255)
                                                                                                                     gACCTTCGACAGCCTCACCCCGGCCACCGCCCCGGCCGGGGCGCCACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGGTCAGGCCGCCCCGCGTGCCGTCGCCAGCAAGGCGGTGCGCCGCCTCCCCGc                 >  6:3224/1‑135 (MQ=255)
                                                                                                                     gACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCCACTGCCCCCCGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCCCCCACCCTGCCGTAGCCCGCAAGGCGTGGCGCAGCATCACCGc                 >  2:293950/1‑135 (MQ=255)
                                                                                                                     gACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCAGGGCGCCACAGCCCGCCGCCGCTGAAGCCGCTGTGGAGCTGGCCGTTCAGGCCGCCCACCGGGCCGTCGCCAGCCAGGCGTGGGGCAGCATCAccc                  >  3:103043/1‑133 (MQ=255)
                                                                                                                     gACCTTCGACAGCCTCAACCCGGCCACCCCCCCGGCCTGGGCGCAACTGCCCGCCGCCGCAGGAGCCGATGTGGAGCTGGCCGTTCAGGCCCCCCAGCGGGCCGTAGTCAGCAAGGCGGGGCGCCGCAACAACGc                 >  8:190187/1‑135 (MQ=255)
                                                                                                                        cttccccAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAc              <  4:202923/129‑1 (MQ=255)
                                                                                                                         ttCGACAGCCTCAACCCGCCCACCGCCCAGCCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc             <  4:152933/135‑1 (MQ=255)
                                                                                                                                gCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg      <  7:300433/135‑1 (MQ=255)
                                                                                                                                gCCGCAACCCGGCCACCGCCCAGCCCTGGGCGCACCTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg      <  6:249964/135‑1 (MQ=255)
                                                                                                                                    cAACCCGGCCACCGCCCCCGCCGGGGCGCCACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGGTGAGGCCGCCCCGCGTGGCGTCGCCAGCCAGGCGTGGCGCCGCCTCACCCCTACCCCCCGCGGCaa  >  2:282135/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TGGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAA  >  KT2440/4135458‑4135724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: