Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,229,020	C→G	6.2%	D116H (GAC→CAC)	CJ019_03968 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,229,020	0	C	G	6.2%	207.4 / 5.3	64	D116H (GAC→CAC)	CJ019_03968	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/34);  new base G (2/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

CCAGGAAGTTGAGCAAGTCCGGCTCATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCTGCGCTCGACCAGCACGAA  >  KT2440/4228891‑4229152                                                                                                                                  |                                                                                                                                     ccAGCAAGTTGAGCCAGTCCGGCTCATCCCCCAGGGCGATCGCCTGCGCCGGCCGCGGCAGGCGCGCCAGCACGGTATTGCAGACAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCt                                                                                                                                 <  3:20435/135‑1 (MQ=255)               aaGTCCGGCTCATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCCCCCTCGCCCCCCAGCAAc                                                                                                                   >  3:15990/1‑135 (MQ=255)               aaGTCCGGCTCATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAAc                                                                                                                   >  8:190793/1‑135 (MQ=255)                 gTCCGGCTCATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCACCGCAGCAGCCAGTCACCCTCGCCACCCAGCAACt                                                                                                                  >  5:124712/1‑134 (MQ=255)                 gTCCGGCTCATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACt                                                                                                                  >  4:83212/1‑134 (MQ=255)                   ccGGCTCATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCa                                                                                                               >  7:270163/1‑135 (MQ=255)                    cGGCTCATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGCTCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCCGCACCTGCaa                                                                                                              >  8:290662/1‑135 (MQ=255)                    cGGCTCATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCaa                                                                                                              <  2:41610/135‑1 (MQ=255)                         cATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCCCCCTCGCCCCCCCGCAACTGCACCGCCa                                                                                                         >  6:92252/1‑135 (MQ=255)                           tCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCAcc                                                                                                       <  6:220871/135‑1 (MQ=255)                              cccAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCACCGCCACCGCt                                                                                                    >  4:221553/1‑135 (MQ=255)                              cccAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCt                                                                                                    >  8:23631/1‑135 (MQ=255)                              cccAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCt                                                                                                    >  4:709/1‑135 (MQ=255)                               ccAGGGCGATAGCCTGCGCCGGCTGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTc                                                                                                   >  2:184609/1‑135 (MQ=255)                                 aGGGCGATAGCCTGCGCCGGCCGGGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCGAGTCACCCTCGGCCCCCAGGAACTGCAACGCCACCGCGCgg                                                                                                 <  8:242147/135‑1 (MQ=255)                                    gCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCCCGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCCGCAACTGCAACGCCACCGCTCGGCCg                                                                                              >  5:160659/1‑135 (MQ=255)                                    gCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCg                                                                                              >  3:115544/1‑135 (MQ=255)                                      gATAGCCGGCGCCGGCCGGGGCAGGCGCGCCAGCACGGCTTTGCAGAACGCCACGGCGGGCAGATCCGGTGCGTAGCAACGCAGCAGCCAGGCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGAt                                                                                            <  3:270664/135‑1 (MQ=255)                                             tGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTgg                                                                                     <  2:220473/135‑1 (MQ=255)                                             tGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTgg                                                                                     <  5:112898/135‑1 (MQ=255)                                                  cggccgCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACg                                                                                >  4:213361/1‑135 (MQ=255)                                                  cggccgCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACg                                                                                >  8:232910/1‑135 (MQ=255)                                                   ggccgCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACgg                                                                               <  5:203592/135‑1 (MQ=255)                                                   ggccgCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACgg                                                                               <  8:24258/135‑1 (MQ=255)                                                   ggccgCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGAGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACgg                                                                               <  8:28944/135‑1 (MQ=255)                                                       gcgGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTAct                                                                           <  8:171286/135‑1 (MQ=255)                                                       gcgGCAGGCTAGCCCGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCCACGCAGCAGCCAGTCACCCTCGACCCCCAGCAACTGCAACGCCCCCGCTCGGCCGAAGCCCTGGCTCCGGttcg                                                                           >  2:235129/1‑132 (MQ=255)                                                          gCAGGCTCGCCAGCACGGCATTGCAGAACGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTActtct                                                                        <  4:164435/135‑1 (MQ=255)                                                                   ccAGCACGTCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCAGCCTCGCCCCCCTGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGAt                                                                 <  5:56211/133‑1 (MQ=255)                                                                    cAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGaa                                                              <  3:172748/135‑1 (MQ=255)                                                                        aCGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAgg                                                          <  7:190793/135‑1 (MQ=255)                                                                           gCATTGCAGAAAGCCACGGCGTGCAGATCCTGGTCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCg                                                       <  7:102889/135‑1 (MQ=255)                                                                           gCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCg                                                       <  4:91839/135‑1 (MQ=255)                                                                           gCATTGCACAACGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGGCACCCGCGCCCCCCACCAACGGCAACGCCACCGCTCGGCCGATGCCCTGACGACGGTACTTCTTCAAGATGAACAGGTCg                                                       <  1:41803/135‑1 (MQ=255)                                                                             aTTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCACCTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGc                                                     <  5:45204/135‑1 (MQ=255)                                                                               tcccccaaGCCCCGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCCCCCGCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCaa                                                   <  5:15242/129‑1 (MQ=255)                                                                                    aaaGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCTCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTcc                                              >  5:31908/1‑135 (MQ=255)                                                                                        ccACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTgcg                                          <  1:184609/135‑1 (MQ=255)                                                                                        ccACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTgcg                                          <  7:40725/135‑1 (MQ=255)                                                                                        ccACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTgcg                                          <  4:171388/135‑1 (MQ=255)                                                                                        ccACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGAGGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTgcg                                          <  1:19318/135‑1 (MQ=255)                                                                                        ccACGGCGGGCAGAGCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCGCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTgcg                                          <  3:191661/135‑1 (MQ=255)                                                                                         cACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATg                                                                >  5:3563/1‑112 (MQ=255)                                                                                         cACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATg                                                                <  6:3563/112‑1 (MQ=255)                                                                                         cACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTgcg                                          >  7:28080/1‑134 (MQ=255)                                                                                         cACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTgcg                                          >  7:34860/1‑134 (MQ=255)                                                                                         cACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTgcg                                          >  7:148001/1‑134 (MQ=255)                                                                                         cACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTACTTCAAGATGAACAGGTCGGCAAGCTCCTgcg                                          >  5:8817/1‑134 (MQ=255)                                                                                                         tgtgCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGc                         >  3:160243/1‑135 (MQ=255)                                                                                                            gCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCt                        <  7:232910/133‑1 (MQ=255)                                                                                                              gTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCt                    <  4:115544/135‑1 (MQ=255)                                                                                                              gTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCt                    >  1:250464/1‑135 (MQ=255)                                                                                                                  cAACGCAGCAGCCAGTGACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAAc                                                             >  6:109954/1‑90 (MQ=255)                                                                                                                  cAACGCAGCAGCCAGTGACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAAc                                                             <  5:109954/90‑1 (MQ=255)                                                                                                                  cAACGCAGCAGCCAGTGACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAAc                                                             <  5:104822/90‑1 (MQ=255)                                                                                                                  cAACGCAGCAGCCAGTGACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAAc                                                             >  6:104822/1‑90 (MQ=255)                                                                                                                   aaCGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACa                                                            >  1:116209/1‑90 (MQ=255)                                                                                                                   aaCGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACa                                                            <  2:116209/90‑1 (MQ=255)                                                                                                                    aCGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCTCCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGCTGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCTGCGCTc              <  1:59408/135‑1 (MQ=255)                                                                                                                    aCGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCTGCGCTc              <  4:160243/135‑1 (MQ=255)                                                                                                                        agcagcCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCTGCGCTCGa            >  1:26939/1‑133 (MQ=255)                                                                                                                          cagcCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCTGCGCTCGACCAg        <  6:255955/135‑1 (MQ=255)                                                                                                                                gTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCTGCGCTCGACCAGCACGaa  <  8:193242/135‑1 (MQ=255)                                                                                                                                gTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCTGCGCTCGACCAGCACGaa  <  7:144605/135‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CCAGGAAGTTGAGCAAGTCCGGCTCATCCCCCAGGGCGATAGCCTGCGCCGGCCGCGGCAGGCTCGCCAGCACGGCATTGCAGAAAGCCACGGCGGGCAGATCCTGTGCGTAGCAACGCAGCAGCCAGTCACCCTCGCCCCCCAGCAACTGCAACGCCACCGCTCGGCCGATGCCCTGGCGACGGTACTTCTTCAAGATGAACAGGTCGGCAAGCTCCTGCGCAACGATGCCAGGCAGCTCGCTGCGCTCGACCAGCACGAA  >  KT2440/4228891‑4229152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑