| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,434,267 | A→C | 31.3% | intergenic (+590/‑259) | dmlR_17 → / → CJ019_04207 | HTH‑type transcriptional regulator DmlR/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,434,267 | 0 | A | C | 31.3% | 36.0 / 8.4 | 30 | intergenic (+590/‑259) | dmlR_17/CJ019_04207 | HTH‑type transcriptional regulator DmlR/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (17/3); new base C (2/8); total (19/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGATTCAGGTTTATGGAAAAAAAGCGGATCAGATGTGATGCGCAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTGAGTTTGCTTGGGAGGACACGATCTGAAACACGACTTGTAGGATTAT > KT2440/4434135‑4434394 | aCAGATTCAGGTTTATGGAAAAAAAGCGGATCAGATGTGATGCGCAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAaca > 2:192989/1‑135 (MQ=255) gggTTTCTGCAAAAAACGCGCTTCAGATGTGAGCCCCAGATGCCGCCGAGGTGTAGGCAGCCGCTGTGGGGGCCGCTGAGCAGGTGTGGCGCGCGGGCGGCCATTTGATACATCAAGTTTCAAAACACGCCTCCa < 8:155151/134‑1 (MQ=255) ggTTTATGGAAAAAAAGCGGATCAGATGTGATGCGCAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAACCACGCCTCCAg > 5:255932/1‑135 (MQ=255) gAAAAAAAGCTGATCAGATGTGATGAGCAGATGCCGCCGAGCTGGATGCAGAAGCAGTTGTTGAAGCTGATCACGATTCTCTCTCGGGACGCCAATTTATAAATGAAGTTTCAAAACACGCCTCCAGCTTTgcga > 5:237751/1‑134 (MQ=255) gAAAAAAAGCGGATCAGATGTGATGCGCAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTgc > 7:92840/1‑133 (MQ=255) aaGCGGATCAGATGTGATGCGCAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTAcc > 1:147225/1‑135 (MQ=255) aaGCGGATCAGATGTGATGCGCAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTAcc > 7:155151/1‑135 (MQ=255) tgATGCGCATAAACCGCCGAGCTGGAGGCAGCCGCAGTGGTTGACGCTGAGCACGTGGCGCGCGCGGGCCGGCAATTGATACATGAAGTTTCAAACCACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTgcg < 2:3803/134‑1 (MQ=255) cgcAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGccc > 7:254822/1‑135 (MQ=255) cgcAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGccc > 4:260223/1‑135 (MQ=255) cgcAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGATGACGCTGAGAACGTGTCGCTCGCGGGCCGTCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGccc > 2:33050/1‑135 (MQ=255) aTGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCg > 2:158469/1‑134 (MQ=255) aTGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCg > 3:223758/1‑134 (MQ=255) cgccgAGCTGTATCCAGCCGCTGTGGTTGCCGCTGAGCACGTGTCGCGGGCGGGCCGGAATTTGAAACATGATGTTCAAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAgc < 7:296470/135‑1 (MQ=255) cgCTGGAGGCCGCCGCTGTGGTTGACGCTGCCCCCGGGGCGCGCGCGGGCCGGAAATTGATACATCAAGATCCAACCCACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCg < 3:117381/134‑1 (MQ=255) aGCTGGAGGCAGCCGCTGTGGTGGCCGCTGCGCACGGGGCGCGCGGGGGCCGCCAATTGATACATCAAGTTTCACACCACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCg < 6:255932/135‑1 (MQ=255) gaggccgCCGCAGTGGTGGACGCTCCGCCGGTGCCGCGCGCGGGCCGTAAATTGATACATGAAGTTCAAAACCACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAAccc < 1:17404/129‑1 (MQ=255) gATGCCGCCGCTGGGGTTGACGCTGCGCACGTGGGGCTCGGGGGCGGGAATTTTATACATCAATTTACAAACCACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAAccc < 1:158309/135‑1 (MQ=255) tGCAGCCGCTGTGGTTGCCGCTGAGCAGGTGGCGCGCGGGGGCCGGCAATTTAAACATCATGTTTCAAACCACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAg < 8:92840/135‑1 (MQ=255) aGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCa > 1:266/1‑135 (MQ=255) gCCGCTGTGGTTCCCGCTGAGCACGTGGCGCGCGCGGGCGGGCATTAGATACATTAATTTAAAAACCACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCaa < 1:187602/135‑1 (MQ=255) tGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACg > 6:115907/1‑132 (MQ=255) tGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACg > 3:185782/1‑132 (MQ=255) aCGCTGCGCACGGGTCGCGCGCGGGCCGGAAATTGATACATGAATTTTCAAACCACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTg < 8:254822/134‑1 (MQ=255) aCGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTg < 4:153778/134‑1 (MQ=255) aCGTGTCGCTCGCGGCCCGGCAAATGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTGAGTTAGCTTg > 1:107306/1‑135 (MQ=255) ctcgcGGGCCGGCAATTGATACATTAAGTTTCAAACCACTCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTGAGTTTGCTTGGGAGGaca > 8:72866/1‑135 (MQ=255) ctcgcGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTGAGTTTGCTTGGGAGGaca > 8:217881/1‑135 (MQ=255) cgcgGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTGAGTTTGCTTGGGAGGACACg > 3:63675/1‑135 (MQ=255) aaGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTGAGTTTGCTTGGGAGGACACGATCTGAAACACGACTTGTAGGat > 2:264144/1‑135 (MQ=255) tttCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTGAGTTTGCTTGGGAGGACACGATCTGAAACACGACTTGTAGGattat > 6:253579/1‑135 (MQ=255) | ACAGATTCAGGTTTATGGAAAAAAAGCGGATCAGATGTGATGCGCAGATGCCGCCGAGCTGGATGCAGCCGCAGTGGTTGACGCTGAGCACGTGTCGCTCGCGGGCCGGCAATTGATACATGAAGTTTCAAAACACGCCTCCAGCTTTGCGCAGTACCTGCGTTGAGTGCGCTGCCCCTCGAAAGCGCCCGAACCCAGCCAATACGGTAGATTGAGTTTGCTTGGGAGGACACGATCTGAAACACGACTTGTAGGATTAT > KT2440/4434135‑4434394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |