Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,440,130 C→A 6.7% Q360H (CAG→CAT)  nicC ← 6‑hydroxynicotinate 3‑monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,440,1300CA 6.7% 161.8 / 2.2 57Q360H (CAG→CAT) nicC6‑hydroxynicotinate 3‑monooxygenase
Reads supporting (aligned to +/- strand):  ref base C (23/30);  new base A (2/2);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

AGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCG  >  KT2440/4440001‑4440255
                                                                                                                                 |                                                                                                                             
aGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCACGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCt                                                                                                                          >  7:139118/1‑135 (MQ=255)
aGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCt                                                                                                                          >  2:5233/1‑135 (MQ=255)
aGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCt                                                                                                                          >  6:145253/1‑135 (MQ=255)
    cAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTag                                                                                                                      <  6:94257/135‑1 (MQ=255)
     aGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTaga                                                                                                                     <  2:6709/135‑1 (MQ=255)
        tAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGcc                                                                                                                  <  5:246728/135‑1 (MQ=255)
          aTGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAg                                                                                                                >  1:286630/1‑135 (MQ=255)
            gCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGgt                                                                                                              >  2:45175/1‑135 (MQ=255)
               ggCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGgtg                                                                                                             >  6:178163/1‑133 (MQ=255)
               ggCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGgtg                                                                                                             <  5:178163/133‑1 (MQ=255)
                   gATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCg                                                                                                       >  8:107742/1‑135 (MQ=255)
                    aTGCCATTGGCGCTGCCGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGt                                                                                                      >  1:38924/1‑135 (MQ=255)
                      gCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGtt                                                                                                     >  4:92457/1‑134 (MQ=255)
                        cATTGGCGCTGACGTTACCACCAGCGAAGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTgg                                                                                                   <  7:250756/134‑1 (MQ=255)
                         aTTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCCTGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGCGCCAGGTGTTGGCGTTGGAt                                                                                                 >  2:115199/1‑135 (MQ=255)
                                cTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGAtt                                                                                          <  2:21461/135‑1 (MQ=255)
                                     gTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCAcc                                                                                     <  8:290784/135‑1 (MQ=255)
                                           accaGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATCCCGATTGCACCTGCGAt                                                                               >  3:118154/1‑135 (MQ=255)
                                                cgacgaAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCg                                                                          >  1:117529/1‑135 (MQ=255)
                                                        gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGc                                                                  <  2:8974/135‑1 (MQ=255)
                                                        gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGc                                                                  <  5:167917/135‑1 (MQ=255)
                                                        gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGc                                                                  <  4:290844/135‑1 (MQ=255)
                                                        gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGc                                                                  <  2:168034/135‑1 (MQ=255)
                                                        gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGc                                                                  <  1:51905/135‑1 (MQ=255)
                                                          gCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGc                                                                                                                           <  2:37050/76‑1 (MQ=255)
                                                          gCTCATGCAGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGTATCTTCCTGGc                                                                                                                           >  1:37050/1‑76 (MQ=255)
                                                              aTGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGtt                                                              >  2:258259/1‑133 (MQ=255)
                                                              aTGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGtt                                                              <  1:258259/133‑1 (MQ=255)
                                                              aTGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTgg                                                            >  4:84467/1‑135 (MQ=255)
                                                              aTGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGAGACCGATTGCCCCTGCGATGCCCGCTCTTTGCGGTTgg                                                            >  2:246287/1‑135 (MQ=255)
                                                               tGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTg                                                             <  3:292646/133‑1 (MQ=255)
                                                                   gccTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATAGCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCa                                                       <  3:113548/135‑1 (MQ=255)
                                                                   gccTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTc                                                        >  3:168822/1‑134 (MQ=255)
                                                                      tCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTTAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATa                                                     >  3:651/1‑134 (MQ=255)
                                                                      tCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGGGGTTGGCTTCATa                                                     >  4:201560/1‑134 (MQ=255)
                                                                          ctcTTTCACGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAAcg                                                <  2:89161/133‑1 (MQ=255)
                                                                             tttCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGa                                             <  2:38924/135‑1 (MQ=255)
                                                                                   gctgctGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCg                                       <  2:117529/135‑1 (MQ=255)
                                                                                             aTAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTc                             <  8:264755/135‑1 (MQ=255)
                                                                                             aTAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTc                             <  3:84336/135‑1 (MQ=255)
                                                                                             aTAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTc                             <  8:160572/135‑1 (MQ=255)
                                                                                                  ggTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACa                        >  3:171973/1‑135 (MQ=255)
                                                                                                  ggTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACa                        <  5:233160/135‑1 (MQ=255)
                                                                                                    tCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGCTACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACACCGCGAACGCCGTGCGGTGGTCGGACAGc                      <  8:81456/135‑1 (MQ=255)
                                                                                                    tCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGc                      <  8:39574/135‑1 (MQ=255)
                                                                                                    tCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGc                      <  8:25023/135‑1 (MQ=255)
                                                                                                       tAGCCGTAAACCCAGGCTGGATCTTCATGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCg                   >  2:35191/1‑135 (MQ=255)
                                                                                                       tAGCCGTAAACCCAGGCGGGATCTTCATGGCTGTAGAGACAGGTGTTGGCGTTGGAGACAGATTGCACCTGCGATTCCCGCTCTTTGCGGGTGGCTTCATACAACGCCAACGCCGTGCGGTGGTCGGACAGCCCg                   >  2:30079/1‑135 (MQ=255)
                                                                                                        aGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCgg                  >  3:198475/1‑135 (MQ=255)
                                                                                                            gTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGtctc              >  6:73723/1‑135 (MQ=255)
                                                                                                              aaaCCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCt            <  1:5233/135‑1 (MQ=255)
                                                                                                                  ccAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTgcag        <  2:286630/135‑1 (MQ=255)
                                                                                                                  ccAGGCTGGATCTTCATGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCTGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACATCCCGGTCTCCTgcag        <  1:30079/135‑1 (MQ=255)
                                                                                                                  ccAGGCTGGATCTTCATGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTgcag        <  1:35191/135‑1 (MQ=255)
                                                                                                                   cAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTgcagg       <  3:162876/135‑1 (MQ=255)
                                                                                                                             cTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCg  >  4:90725/1‑130 (MQ=255)
                                                                                                                             cTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCg  <  3:90725/130‑1 (MQ=255)
                                                                                                                                 |                                                                                                                             
AGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCG  >  KT2440/4440001‑4440255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑