Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,670,287 C→T 7.5% R645Q (CGG→CAG)  adiA ← Biodegradative arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,670,2870CT 7.5% 169.1 / 3.5 53R645Q (CGG→CAG) adiABiodegradative arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (19/30);  new base T (2/2);  total (21/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01

TACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGCCCCCACACTGGGCAGGCAGCTGCTGAGTGGGG  >  KT2440/4670155‑4670419
                                                                                                                                    |                                                                                                                                    
tACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGc                                                                                                                                    >  4:196601/1‑135 (MQ=255)
       ccAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAgct                                                                                                                             <  7:11602/135‑1 (MQ=255)
             tCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTgg                                                                                                                       <  4:47341/135‑1 (MQ=255)
             tCACCGCAGCCACACGCCCCAGCAATGCCTGGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCGTGGACGGGCTGACTGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCATCTTCTTgt                                                                                                                       <  2:33585/135‑2 (MQ=255)
                              cccAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCgg                                                                                                      <  7:164008/135‑1 (MQ=255)
                               ccAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGt                                                                                                     <  8:141338/135‑1 (MQ=255)
                               ccAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGt                                                                                                     <  2:4294/135‑1 (MQ=255)
                                cAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCCGCTGCTTGGCCGTGGCATTGGCggggga                                                                                                    >  1:155538/1‑133 (MQ=255)
                                 aGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTa                                                                                                    >  3:263563/1‑134 (MQ=255)
                                 aGCAACGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCCTCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAg                                                                                                   <  4:209431/135‑1 (MQ=255)
                                   cAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCa                                                                                                 <  5:290155/135‑1 (MQ=255)
                                         cTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATATGCCTGGGCCGGGCTGACGGCCACCTCCGGCACACGGGTGAACAGCCGCTTCAGCTGCGTGGCCGTGGCATTGGCGCGGTGGCAGTCag                                                                                            >  8:132147/1‑133 (MQ=255)
                                           cGATCGGCACCGCCTCCACTTCGCCCCGCCCCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTa                                                                                         >  3:274901/1‑135 (MQ=255)
                                           cGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTa                                                                                         >  3:237908/1‑135 (MQ=255)
                                           cGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTa                                                                                         >  3:240373/1‑135 (MQ=255)
                                           cGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTa                                                                                         >  3:41935/1‑135 (MQ=255)
                                           cGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCCGCACACGCGTGAACAGCCGCTTCAGCTGCTTGGCCGTCGCCTTGGCGCGGTAGAAGTCAAGTa                                                                                         >  7:114034/1‑135 (MQ=255)
                                             aTCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTaa                                                                                        <  2:172746/134‑1 (MQ=255)
                                                  cACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTc                                                                                  <  4:35756/135‑1 (MQ=255)
                                                      gCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGcac                                                                              <  3:196601/135‑1 (MQ=255)
                                                      gCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGcac                                                                              <  4:240373/135‑1 (MQ=255)
                                                          ccACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGATTGGCCGTGGCCTTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGt                                                                          >  8:28658/1‑135 (MQ=255)
                                                            aCTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTc                                                                         <  4:49274/134‑1 (MQ=255)
                                                                  ccACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAg                                                                   <  4:237908/134‑1 (MQ=255)
                                                                   cACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGc                                                                 >  6:80275/1‑135 (MQ=255)
                                                                   cACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGc                                                                 <  1:9137/135‑1 (MQ=255)
                                                                        aCCAGCTTGGCATAGGCCGGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGGGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATg                                                            <  2:144964/135‑1 (MQ=255)
                                                                           aTCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGccc                                                         <  3:58580/135‑1 (MQ=255)
                                                                               tGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTg                                                       >  5:266655/1‑133 (MQ=255)
                                                                               tGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTCGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCccctgccctg                                                       >  7:132224/1‑133 (MQ=255)
                                                                                  cATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAg                                                   <  2:219479/134‑1 (MQ=255)
                                                                                    tAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAgcg                                                 >  8:190990/1‑134 (MQ=255)
                                                                                        ccTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAgg                                            <  8:197410/135‑1 (MQ=255)
                                                                                            ggCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTc                                        <  4:247022/135‑1 (MQ=255)
                                                                                            ggCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTc                                        <  2:10067/135‑1 (MQ=255)
                                                                                            ggCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGGCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTc                                        <  1:195950/135‑1 (MQ=255)
                                                                                             gCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCg                                       <  6:86189/135‑1 (MQ=255)
                                                                                               cGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCg                                       >  3:115906/1‑133 (MQ=255)
                                                                                               cGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCg                                       >  1:95998/1‑133 (MQ=255)
                                                                                                 ggCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAg                                   <  7:8326/135‑1 (MQ=255)
                                                                                                    tGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGccc                                >  1:131853/1‑135 (MQ=255)
                                                                                                     gACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGcccc                               >  3:47819/1‑135 (MQ=255)
                                                                                                           cACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGCCCCCACACt                         <  1:39134/135‑1 (MQ=255)
                                                                                                                  ggCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGCCCCCACACTGggcagg                  <  4:201111/135‑1 (MQ=255)
                                                                                                                     aaaCGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGCCCCCACACTGGgcaggcag               <  5:18948/135‑1 (MQ=255)
                                                                                                                      aaCGGGTGAACAGCTGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAg                                                                                                   >  7:225399/1‑50 (MQ=255)
                                                                                                                      aaCGGGTGAACAGCTGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAg                                                                                                   >  7:229307/1‑50 (MQ=255)
                                                                                                                      aaCGGGTGAACAGCTGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAg                                                                                                   <  8:225399/50‑1 (MQ=255)
                                                                                                                      aaCGGGTGAACAGCTGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAg                                                                                                   <  8:229307/50‑1 (MQ=255)
                                                                                                                           gTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGCCCCCACACTGGGCAGGCAgctgc           >  1:171264/1‑133 (MQ=255)
                                                                                                                            tGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGCCCCCACACTGGGCAGGCAGCTGCTGa        <  5:80275/135‑1 (MQ=255)
                                                                                                                            tGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACGGGTCGCACAGGGCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGCCCCCACACTGGGCAGGCAGCTGCTGa        <  3:224065/135‑1 (MQ=255)
                                                                                                                                  gCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGCCCCCACACTGGGCAGGCAGCTGCTGAGTgggg  <  2:95998/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
TACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGCCACCTCCGGCAAACGGGTGAACAGCCGCTTCAGCTGCTTGGCCGTGGCATTGGCGCGGTAGCAGTCATGTAACTGGTCGCACAGGTCGCGCAGGCCCATGCCCTGGTAGCGCAAGGCGTCGGCAGCCCCCACACTGGGCAGGCAGCTGCTGAGTGGGG  >  KT2440/4670155‑4670419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑