Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,749,286 T→G 26.8% S294A (TCG→GCG)  macB → Macrolide export ATP‑binding/permease proteinMacB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,749,2860TG26.8% 23.2 / 5.7 29S294A (TCG→GCG) macBMacrolide export ATP‑binding/permease proteinMacB
Reads supporting (aligned to +/- strand):  ref base T (9/12);  new base G (3/5);  total (12/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-01

CGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCG  >  KT2440/4749157‑4749413
                                                                                                                                 |                                                                                                                               
cGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtg                                                                                                                            <  8:240377/135‑1 (MQ=255)
     tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCGTGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCgggggtgggggt                                                                                                                       >  1:294014/1‑135 (MQ=255)
     tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtggtggt                                                                                                                       >  2:227475/1‑135 (MQ=255)
     tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtggtgg                                                                                                                        >  2:50505/1‑134 (MQ=255)
     tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCgggggtggt                                                                                                                       >  3:123051/1‑135 (MQ=255)
           ccAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGtt                                                                                                                 <  1:146117/135‑1 (MQ=255)
                aCGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGGGGTGGGGATGTTGGGTg                                                                                                            >  2:146117/1‑135 (MQ=255)
                       cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGt                                                                                                       >  8:3168/1‑133 (MQ=255)
                       cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGt                                                                                                       >  4:87078/1‑133 (MQ=255)
                       cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGGGGTGGGGGTGTTTGGTGTgggg                                                                                                       >  7:137095/1‑132 (MQ=255)
                            atggTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTTATGTTGGCTGTCGGTGAAGgca                                                                                                <  8:58998/133‑1 (MQ=255)
                              ggTGAACTGCTCGAAGCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGgcagc                                                                                              <  8:163955/135‑1 (MQ=255)
                                 gAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCaaa                                                                                           <  7:184929/135‑1 (MQ=255)
                                   aCTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACg                                                                                         >  8:95651/1‑135 (MQ=255)
                                           aaTCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTaa                                                                                 <  3:290869/135‑1 (MQ=255)
                                                 tGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGCTGGCGGTGAAGGCAGCAAACGCCAGGTAATGGcac                                                                           <  4:123051/135‑1 (MQ=255)
                                                 ggCAGGGGGCTGGGCGAGTGAGGTGGATCAACCGCGCCCGCACGGCAGTGACCCGGCGGGGAAGCATCATGGGAGTGGCGGCGGGGGTGGTGATGTTGGCTGTCGGTCAAGCCAGCAAACGCCAGGTAATGGcac                                                                           <  2:294014/134‑1 (MQ=255)
                                                    aGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGa                                                                        <  3:264222/135‑1 (MQ=255)
                                                              gCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTGGGTGGTGGTGATGTTGGCTGTCGGTGCAGGCCGCCAACGCCAGGTAATGGCACAGAAGgccgccgt                                                              >  4:297864/1‑133 (MQ=255)
                                                                    gATGTTCATCACCCGCTTCCGCACGCAACTGACCCTGCTCGGAATCATAATCGGTGTCGCGGCGGTGGTGGTTATGTTGGCTGTCGGTGAAGGCAGAAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCt                                                         <  5:95565/134‑1 (MQ=255)
                                                                                        gCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAt                                     <  7:3168/134‑1 (MQ=255)
                                                                                         cACGGCACTGCACATGCTCGTAATCATCATCTGAGTCGGGTGGGTGGTGGTGTTGTTGGCTGTGGTTGATGGCAGAAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                   <  1:148430/135‑1 (MQ=255)
                                                                                                           cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGGTGTGGGTGAAGGGCGCAAACCCCCGGTTATGGGCCAGATGGCCCCCTTCGGCTCGAACCTCCGATAACGCAATGGCCGCCCCCCCACCCGgcg                 >  5:143716/1‑135 (MQ=255)
                                                                                                           cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAAAGGCACAGATGTCCGCCTTCGGCTCCAACATACTATACCTCCATTGCCCCCCCCCGAcccatcg                 >  7:180444/1‑131 (MQ=255)
                                                                                                           cGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGGTGTAGGGGTAGGCCGCCAACGCCCGGGTATGGCCCAGATGGCCCCCGTCGGCGGCAACAACCGATACCTCAATGGCCGCCCCCCGCCCCcgcg                 >  3:264195/1‑135 (MQ=255)
                                                                                                                   tcatcGGAGTCGTGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCACGAACCg          <  8:96054/134‑1 (MQ=255)
                                                                                                                         gAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc   <  7:95651/135‑1 (MQ=255)
                                                                                                                         gAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGGAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc   <  5:277693/135‑1 (MQ=255)
                                                                                                                         gAGTCGCGGGGGGGGTGGTGAGGGTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgc   <  8:129303/135‑1 (MQ=255)
                                                                                                                          aGTCGCGGCGGTGGTGGTGATGTTGGCGGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgcg  <  4:264195/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                               
CGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCG  >  KT2440/4749157‑4749413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: