Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,789,443	A→C	31.1%	V230G (GTG→GGG)	dat ←	Diaminobutyrate‑‑2‑oxoglutarateaminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,789,443	0	A	C	31.1%	42.7 / 6.9	53	V230G (GTG→GGG)	dat	Diaminobutyrate‑‑2‑oxoglutarateaminotransferase
Reads supporting (aligned to +/- strand):  ref base A (21/13);  new base C (13/4);  total (35/18)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCA  >  KT2440/4789318‑4789575                                                                                                                               |                                                                                                                                      cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCTCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGAAACAC‑TTCGATGATCACGGCGGCGGGCAGCGGCACCCCGCctt                                                                                                                              >  4:265460/1‑135 (MQ=255) cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCttt                                                                                                                              >  6:29932/1‑135 (MQ=255) cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCgtt                                                                                                                              >  5:129597/1‑135 (MQ=255)      aTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑ATCGAGGATCACGGCGGCGGGCACCGCCACGCCGCTTTACGGa                                                                                                                         >  3:137231/1‑135 (MQ=255)          acgccacgccCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGGAGCGGCCCGCCGCCTGCCCGATCgc                                                                                                                     >  8:268268/1‑134 (MQ=255)                   ccgccTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGt                                                                                                            >  8:106044/1‑135 (MQ=255)                             ggTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGAGCAGGAGGTTTTCCAGGTa                                                                                                  >  3:61464/1‑135 (MQ=255)                               tAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCCCCCTGCACCAC‑TTCGAGGAGCACGGCGGCGGGCCGCCGCCCGCCGCTGTCCGGATCGCGCCGCCTGGTTTGCAGGGAAt                                                                                                >  6:192000/1‑135 (MQ=255)                                  tGCGCCGCACGCCTTTGAGCCATGCGATGTGGCCCGTAACCACCCCGCCCACCCCCTCCCCCAA‑TTCGAGCACCAGGGCGGGGGGCAGCGCCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa                                                                                             <  6:132047/135‑1 (MQ=255)                                  tGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCCCCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa                                                                                             <  7:75638/135‑1 (MQ=255)                                       cgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTg                                                                                        >  1:162515/1‑135 (MQ=255)                                       cgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCA‑CCCGCCCTCACCCTGCACCAC‑TTCGAGGCTCACGGCGGCGGGCCGCGGGCCGCCGCGTTCCGGATCGCGCCGCAGGGTTTGCAGGTGATGGAGGTTgt                                                                                       >  5:194142/1‑134 (MQ=255)                                        ccACGCCCTTGAGCCATTCGATGGCGGCCGCAACCACCCCCCCCTCCCCCGCCACCAC‑TGCGAGCACCCCGGCGGGGGGCCGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTgg                                                                                       <  2:162515/134‑1 (MQ=255)                                          aCGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCCGCATGCCGCCTTCCGGATCGAGCCCGCGGTGTTCCAGGGAAATCATGTTgggc                                                                                     >  1:267668/1‑133 (MQ=255)                                            gCCCTTGAGCCATTCGATGTCGGCCGGAACCCCCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCCGCCCGCCCCGTACCGGTTAGAGCAGCACGGTTTACACGTATTGCAGGTTgggcga                                                                                   >  5:246209/1‑131 (MQ=255)                                                 tGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCGTTCCGGGTCGAGCAGCCGGTTTTCCAGGTAATGCAGGTTTGCCGTG‑Accc                                                                             >  4:264717/1‑135 (MQ=255)                                                   cccccttCGAGGGCGGCCGCACCCCCCCCGCCCGCCCCCTGCACCAC‑TGCGAGGACCCCGGCGGCGGGCAGCGCCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑Accc                                                                             <  7:70113/128‑1 (MQ=255)                                                     ccATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCCGCACACCCCATTACGGTTCGCGCAGCATCTTTTCCAGGTAATGCAGGATTGCCTTGTACCCCgc                                                                          >  4:204066/1‑134 (MQ=255)                                                       tttCGAGGTCGCCCGCAACCCCCCCGCCCTCCCCCTGCACAAG‑TGCGAGGACACCGGCGGCGGCCTGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCt                                                                       <  2:212785/134‑1 (MQ=255)                                                       aTTCGAGGGCGGCCGCACCCACCCC‑CCCGCCCCCCGGCCCACGTTCGAGCAGCCGGGCGCCGGGCCGGGCCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCt                                                                       <  3:36767/135‑1 (MQ=255)                                                           gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGCGCCCCCCCGGTGCCGCGCCAGCAGCCCGTGTTACACGTAATGCAGCTTGGCCTTT‑ACCCCCGCCCCAcc                                                                   >  6:174963/1‑135 (MQ=255)                                                           gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCCCGGCCCGCCCCCTTACGGCTAGCGCACCAGGGTTTGAAGGTTATGCAGGTTTGCCGTG‑ACCCCGcccgcccc                                                                   >  2:228841/1‑130 (MQ=255)                                                           gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGCTCGAGCAGCAGGGTTTCCAGGTAATGCAGGTTGGCCTTT‑ACCCCGGCCTCAcc                                                                   >  8:248505/1‑135 (MQ=255)                                                           gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGCGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCCGCCTcccc                                                                   >  6:140482/1‑135 (MQ=255)                                                           gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGATCGAGCAGCAGGTTTTGCAGGTAATGCAGGTTGGGCTTG‑ACCCCGGccgcccc                                                                   >  3:19592/1‑130 (MQ=255)                                                           gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGGTTTGCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCAcc                                                                   >  8:249684/1‑135 (MQ=255)                                                           gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGCGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCAcc                                                                   >  7:228854/1‑135 (MQ=255)                                                           gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCCGCACGCCGCCTTGCGGATCGAGCAGCAGGTTTTACAGGTAATGCAGGTTGGGCATT‑ACCCCCGCCTAAcc                                                                   >  7:184959/1‑135 (MQ=255)                                                                     ggAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGGCGCGCCGCGGTGCGGCGCGCGCCGCCGGGTTTGCAGGTTATGCAGGGTGGGCGTG‑GCCCCCGCCGCCCCCCCCCGGccc                                                         >  2:128526/1‑134 (MQ=255)                                                                     ggAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCGTTCCGGGTCGAGCAGCAGGTTTTCCAGGGAATTGAGGTTGGGCTTG‑ACCCCGGCCTCACCCCCCAGGccc                                                         >  8:13302/1‑134 (MQ=255)                                                                               cGCCCACACCCTCCACCCT‑TGCGTGCATCACGGGGGCGGGCCGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGc                                               <  8:282734/135‑1 (MQ=255)                                                                                gCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATAGAGCAGCAGGGTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGCCCACACCCCCCAGGCCCAACGGGCAGCg                                              >  7:282734/1‑135 (MQ=255)                                                                                      ccccgccaccag‑TTCGCGGTACCCGGCGGCGGGCTGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                        <  4:135511/129‑1 (MQ=255)                                                                                         cTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCTTTCCGGGTCGCGCAGCAGGTTTTCCAGGTTATGCCGGGTGGGCGTG‑ACCCCGGCCTCACCCCCCCGGCCGACCCGGCCGCGGTAGTCGTa                                     >  5:19902/1‑135 (MQ=255)                                                                                             accac‑TTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCGTTGCGGATCGCGCAGCAGGTTTTGCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCGCCCCGCCCACGCCGCACGGGCCGCGGGAGGCGGACggg                                 >  2:95773/1‑135 (MQ=255)                                                                                              accctttCGAGGATCCCGGCGGGGGGCAGCGGCCGCCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                   <  7:192581/128‑1 (MQ=255)                                                                                               cac‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACggg                                 >  8:154004/1‑133 (MQ=255)                                                                                               cac‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTTGCCTTG‑ACCCCCGCCTCACCGCCCCGGCCGCACCGGCAGCGGTAGTGGTACggg                                 >  3:14162/1‑133 (MQ=255)                                                                                                ac‑TTCGAGGAGCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTaa                              <  6:129597/135‑1 (MQ=255)                                                                                                    tCGAGGATCACGGCGGCGGGGAGCGGCACCCCGCTTTACGCATAGAGCACCAGGTTTTCCAGGTAATGCAAGTTGGCATTT‑AAACCCGCCACACCCCCCAGGCCGAACCGGCAGCGGTAGACGTACGGGTAAgcc                           >  6:95773/1‑133 (MQ=255)                                                                                                     cGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCATG‑ACCCCGGCCTCACCGCCCAGGCCGAAAGGGCAGCGGTAGTCGTACGGGGAAGGCa                          >  3:54438/1‑135 (MQ=255)                                                                                                      gAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCCGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCCCCCTCCCCGCCCAGGCCGCACCGGCAGCGGTAGTCGTACGGGTAAgg                            >  3:39401/1‑132 (MQ=255)                                                                                                      ctgaaTCCCGGGGGGGGGCTGCGGAAGGCGGATTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt                         <  1:86208/131‑1 (MQ=255)                                                                                                        ggATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGa                       >  7:3361/1‑135 (MQ=255)                                                                                                         gcgcccggcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGGTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGaa                      <  7:149192/130‑1 (MQ=255)                                                                                                           tCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACt                    <  7:106044/135‑1 (MQ=255)                                                                                                              cggccgcggGCACCGCCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAAGGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCa                 <  2:252966/135‑1 (MQ=255)                                                                                                               ggcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCAc                >  7:29592/1‑135 (MQ=255)                                                                                                                   gcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCg            <  4:145767/135‑1 (MQ=255)                                                                                                                    cggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGt           >  4:107120/1‑135 (MQ=255)                                                                                                                     gggCCGCGGCTCGCGGCTTTCCGGATCGAGCAGGAGGTGTTCCAGGTAATGCAGGTTGGCGTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGGGGTAGTCGTACGGGTAATGCATGAACTGCACGCCGtt          <  7:11428/135‑1 (MQ=255)                                                                                                                     gggCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGtt          <  3:153990/135‑1 (MQ=255)                                                                                                                             gaaCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCa  <  1:193941/133‑1 (MQ=255)                                                                                                                             gCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCa  <  5:151899/135‑1 (MQ=255)                                                                                                                               |                                                                                                                                      CGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCA  >  KT2440/4789318‑4789575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑