| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 382,476 | A→C | 43.9% | E11A (GAG→GCG) | soxD → | Sarcosine oxidase subunit delta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 382,476 | 0 | A | C | 43.9% | 7.9 / 2.4 | 26 | E11A (GAG→GCG) | soxD | Sarcosine oxidase subunit delta |
| Reads supporting (aligned to +/- strand): ref base A (4/9); new base C (7/5); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCACTGGCCGCGCCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTT‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAACCCGCGCGGTATTCACC > KT2440/382345‑382607 | acccACGGGCCGCGCCGTTCCCCCTGGACCGTTTCAACAAGGGCGCACTGAGCCACGACCCCGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCt < 5:193356/133‑1 (MQ=255)aTCCACTGGCCGCGCCGTTCTCCATGGACCGTTTCTAAAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCt < 4:18695/135‑1 (MQ=255) gTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCCCTAACCGGAGACCCCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCGCTTCGCTCCGAAGAagag > 8:240054/1‑135 (MQ=255) gccccATGCAGCTTTTCAACACGGGCGCACTGTGCGACAACCCGGGCGCCGCCGCCGTCGCCCACTAGCGGGACACACCGTCTTGTTGCATATTTTCTGGCCCCACGGCGCCGCGCTGCGCTCCTAAGAAGAGtc < 6:243027/132‑2 (MQ=255) ccGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCAGCCGTCGCCCACTAACCGGAGACCCCGTCATGTTTCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGCATACTT‑CCCCCCct > 6:92414/1‑133 (MQ=255) gTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCGCTGCGCTCCGCAGAAGAGTT‑ACACGCCGCTgg > 8:56104/1‑135 (MQ=255) gTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCCCTGCGCTCCCAAGAAAAGGT‑CCAccccacagg > 8:290393/1‑130 (MQ=255) gTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTT‑CCACGCCTCTgg > 7:97165/1‑135 (MQ=255) gTTTCTACAACGGCGAACAGATCGACGAACACGGCGCCGCCGCCGTCGCCCCCTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGTGCTGCGCTCCCAAGAACAGTT‑ACCCCCctctct > 6:203250/1‑133 (MQ=255) tACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCGCAGCGCGCCCAAGCAGAGTTACCAC‑CCACTGGcccgc > 3:9458/1‑132 (MQ=255) agtGATCGCGGACCCCGGCGCCGCCGCCGTCGCCCACTACCCGGCCACACCGTCAGGTTCAATATTTTCTGCCCCCACTGCGGCGCGCTGCGCTCCGAACAAGCGTT‑CCACGCCTCTGGCCAGGCGCACATTgcc < 2:234/133‑1 (MQ=255) tCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCGCGGCGCTCCGCAGAAGAGTT‑CCACGCCTCTGGCCCGGCGCACATTgcccgccc > 7:27830/1‑135 (MQ=255) acacGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTT‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGAc < 1:13743/135‑1 (MQ=255) gccgccgTCGCCCACTAACCGGAGACACCTTCTTGTTGCATATTTTCTGTCCCCACTGCGGCGAGCGGCGCTCCGAAGAAGAGTT‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCt < 4:183509/135‑1 (MQ=255) cgccGCCCACTAACCGGAGACACCGTCAGGTTGGATTTTTTCTGTCCCGACTGCGCGGAGCTGCGCTCCGAAGACGAGTT‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTcc < 4:274679/132‑1 (MQ=255) ggcGCCAACAACCCGGACACCCCGTCTTGTTGCATTTTTGCGGCCCCCCCGGCGCCGAGCGGCGCCCCGAAGAAGGGTC‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCcg < 5:249521/133‑1 (MQ=255) cGCCCACTACCCGGAGACACCGGCAGGTTGCATATTTTCTGGCCCCACCGCGGCGCGCGGCGCGCCGAAGAAGAGTG‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCcgac < 6:86/135‑1 (MQ=255) acccGGAGACACCGTCATGTTGCATATTTTCTGCCCCCACTGCGGCGAGCGGCGCTCCGAAGAAGAGTG‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTg < 4:9458/133‑1 (MQ=255) cGGATACACCGTGATTTGGCATATTTTCTGGCCCCCCTGCGGCTCGCTGCGCGCCGAAAACGCGTG‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTgggg < 6:232152/135‑1 (MQ=255) cacGGTCATGTTGCATATTTGCTGTCCCCAGTCCGTCGCGCTGCGCACCGAAGACGAGCT‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTAcc < 6:146635/133‑1 (MQ=255) aTGTTGCATATTTTCTGTCCCCACTGCGGCGCGCTGCGCTCCGAAGAAGAGTT‑CCACGCCTCTGGCCCGGCGCACATTGCCCGCCCGCTGGCCCCTAACGCCTGCTCCGACGAGGCGTGGGGTACCTAAATGTTc > 6:48031/1‑135 (MQ=255) atTTTCTGTCCCCACTGCGGCGCGCTGCGCGCCGAAGAAGAGTT‑CCCCCCCTCTGGCCCCGCGCACATAGCCCCCCCCCGGGCCCCTAACGCCGGCTCCGACCCGGAGTGGGGTACCGACATGTTCTACCGTgc > 8:25439/1‑133 (MQ=255) cTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTT‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAAccc > 7:40463/1‑135 (MQ=255) tGTCCCCACTGCGGCGAGCTGCGCTCCGACGACGCGTG‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAAccc < 5:141261/134‑1 (MQ=255) cccACTGCGGCGAGCTGCGCTCCGAAGAAGAGTT‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGCCGAGGAGTGGGGTACCTACATGTTCTACCGGGCCAACCcgcgcg > 1:160162/1‑135 (MQ=255) gcgAGCTGCGCTCCGAAGAAGAGTC‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAACCCGCGCGGTATTCAcc < 6:98515/135‑1 (MQ=255) | ATCCACTGGCCGCGCCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTT‑CCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAACCCGCGCGGTATTCACC > KT2440/382345‑382607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |