Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,874,567 A→C 35.8% intergenic (+13/‑174) CJ019_04568 → / → uacT_1 hypothetical protein/Uric acid transporter UacT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,874,5670AC35.8% 26.4 / 2.5 31intergenic (+13/‑174)CJ019_04568/uacT_1hypothetical protein/Uric acid transporter UacT
Reads supporting (aligned to +/- strand):  ref base A (10/9);  new base C (7/5);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGC  >  KT2440/4874436‑4874696
                                                                                                                                   |                                                                                                                                 
gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGCCCGCACCTCGCCCgg                                                                                                                                 >  8:123445/1‑134 (MQ=255)
   ttCGGGCCCATGGGGTTGCCAGCCAGAACATGCCGGTGGGAAAAATAACCCAGATGACAACCTAAGCGCCCACGCGGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc                                                                                                                             <  8:50418/135‑1 (MQ=255)
               cGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAg                                                                                                                 >  2:184447/1‑135 (MQ=255)
                  ttGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCACGGGACCTGACCCCACCGCGCCCGGCCAcccccccccccacg                                                                                                                >  1:265367/1‑129 (MQ=255)
                      cacccagAACATGCCGCTGGGCAAAATCACCCAGATGACCCCCGAACCCCGCAAGCTCTTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCa                                                                                                          <  6:116506/132‑1 (MQ=255)
                                     gCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCCCCTCGCCAGGCATCCCGCCCCCCAAGGGAATCCCCTTCCCCAGCAggc                                                                                           >  8:134073/1‑134 (MQ=255)
                                         ggCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAAATGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAAACACCTTCCCCTGCAGGAGCgg                                                                                       >  8:293325/1‑135 (MQ=255)
                                         gcaaacaacACCCAGATGCCCACCCAACCGCCCACGCTCTGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCgg                                                                                       <  2:7329/132‑1 (MQ=255)
                                             aCATCCCCCATATCCCCACCCACGCCCGCACGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCtt                                                                                   <  3:230477/135‑1 (MQ=255)
                                                 cACCCAGATGACCACCGAAGCGCGCAAGCTCGTCGGCGACTGGATCCGCCAAGGCGCCCCCCGGCACAGCCCCCACCTCCCCAGGCAACACCCCCCCCAAGGGCATCCACATCCCCTGGCGGATCGGGGTGGccc                                                                               >  3:102761/1‑135 (MQ=255)
                                                           accaccGAAGAGCGCAAGCTCGTCGGCGACTGGCACGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCACGCAACCCGCCCCCCAAGGGAATCACCTACCCCAGCAGGAGCGGGCGTGCCCGCGCAGAGGc                                                                     >  1:105992/1‑135 (MQ=255)
                                                           accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCACCTTCCCCGGCAGGAGCGGGCTTGCCCGCGAAGAGGc                                                                     >  8:29741/1‑135 (MQ=255)
                                                           accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATACCCAAAGGCGCCCACGTCAACTCACACCACCTCGCCAGGCCAACCCCCCCCCAAGGGAATCCACATACCCAGCAGGACCGGGCATTGCCGCGAAGAAGc                                                                     >  4:108790/1‑135 (MQ=255)
                                                                   agagCGCAAGCTCGTCGGCGACTGGCTCGCCAAACGCGCCCAGGTCCACGGACAGCACCGCGCCCGGCCACCCCCCCCCCAAGGGCATCCCCTTCCCCCGCACGCGCGGGCTTGGCCGCGCAGCGGCCCCTAGcc                                                             >  6:218305/1‑135 (MQ=255)
                                                                         cacgggcgtcgGCGACTGGAGCCCCAACGGCGCCCAGGACAACTCACAGCACCTCCCCCGGCAACCCGCCCCCCCAGGGAAACACCTTCCCCGGCAGGCGCGGGCTTGCCCGCTACGAGGCCGATAGCCCCAGcc                                                       <  4:296793/129‑1 (MQ=255)
                                                                                    gCGACTGGGTAGCCCAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCCACCCGCCCCCCAAGCGAAGCACCATCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGAAAGCCCCCTCCTCAAGCTCCaa                                            >  2:31415/1‑134 (MQ=255)
                                                                                    gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCCACCCGCCCCCCAAGGGCATCACCTTCCCCTGCAGGCGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCCGCCTCAAGCTCCAt                                            >  3:93738/1‑135 (MQ=255)
                                                                                     agACTGGAGCGCCAATGGCGGCACGGTCATATGAACGAACCGCCCCCGGCACCCCGCCCCCCTGGTGATTCAGGTGCCCCTGCTGGAGGGGGGTGCCGCGGTATGAGGACGATAGCCCCAGCCTCAAGCTCCAtg                                           <  6:144699/134‑1 (MQ=255)
                                                                                         gggttCGCCAATGGCGCCCTGGTCAACTGACCGACCCGCGCCAGCCACCCCGCCCCACATGGTAAACCCTTTCCCAGGCAGGCGCGGGCTTGCCGGCTATGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGtt                                       <  4:62342/131‑1 (MQ=255)
                                                                                            agcGCCAACGGCGCCCAGGTCACCTGACAGCACCGCCCCCGGCACCCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                    <  8:291539/133‑1 (MQ=255)
                                                                                             tCGCCAAAGGCGCCCAGGTCAACTGACAGCCCCTCGCCCGGCCACCCCCCCCCCCACGGAATCACCTTCCCCGGCCGGCGCGGGCGTGCCCGCGAAGACGCCGATAGCCCCCGCCGCAAGCTCCAAGTGTTaag                                    >  4:148064/1‑132 (MQ=255)
                                                                                                ccAACGCCGCCCTGGTAAACTTACCCCACCGCCCCAGGCAACCCGCCCCCCAAGGTAATCACCTTCCCCGGCAGGTGCGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac                                <  2:235767/135‑1 (MQ=255)
                                                                                                  aaaGGCGCCCAGGGAAACTTACACCACCTCGCCAGGCAACCCGCCCCCCAAGGGAAGCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaacaa                              <  4:172230/135‑1 (MQ=255)
                                                                                                               tCAACTGACAGCACCTCGCCAGGCACCCCGCCCCCCACGGGAATCCCCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                 <  4:62795/135‑1 (MQ=255)
                                                                                                               tCAACTCCCAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAAGCACCTGCCCGTGCAGCAGCGGGCTGGCCCGCGACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                 <  7:231880/135‑1 (MQ=255)
                                                                                                               caaaCTTACACCACCTCGCCCGGAAACCCCCCCCCCACGGAAACCACCTACCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                 <  8:297669/133‑1 (MQ=255)
                                                                                                                      aCAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAgg          >  1:151811/1‑135 (MQ=255)
                                                                                                                           aCCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAt       >  6:104223/1‑133 (MQ=255)
                                                                                                                           aCCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAt       >  4:146986/1‑133 (MQ=255)
                                                                                                                           aCCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAt       >  3:121948/1‑133 (MQ=255)
                                                                                                                              tccccAGGCAACCCGCCCCCCAAGGGAATAACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc  <  4:245594/132‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGC  >  KT2440/4874436‑4874696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: