Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | KT2440 | 4,874,567 | A→C | 35.8% | intergenic (+13/‑174) | CJ019_04568 → / → uacT_1 | hypothetical protein/Uric acid transporter UacT |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | KT2440 | 4,874,567 | 0 | A | C | 35.8% | 26.4 / 2.5 | 31 | intergenic (+13/‑174) | CJ019_04568/uacT_1 | hypothetical protein/Uric acid transporter UacT |
Reads supporting (aligned to +/- strand): ref base A (10/9); new base C (7/5); total (17/14) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGC > KT2440/4874436‑4874696 | gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGCCCGCACCTCGCCCgg > 8:123445/1‑134 (MQ=255) ttCGGGCCCATGGGGTTGCCAGCCAGAACATGCCGGTGGGAAAAATAACCCAGATGACAACCTAAGCGCCCACGCGGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc < 8:50418/135‑1 (MQ=255) cGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAg > 2:184447/1‑135 (MQ=255) ttGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCACGGGACCTGACCCCACCGCGCCCGGCCAcccccccccccacg > 1:265367/1‑129 (MQ=255) cacccagAACATGCCGCTGGGCAAAATCACCCAGATGACCCCCGAACCCCGCAAGCTCTTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCa < 6:116506/132‑1 (MQ=255) gCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCCCCTCGCCAGGCATCCCGCCCCCCAAGGGAATCCCCTTCCCCAGCAggc > 8:134073/1‑134 (MQ=255) ggCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAAATGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAAACACCTTCCCCTGCAGGAGCgg > 8:293325/1‑135 (MQ=255) gcaaacaacACCCAGATGCCCACCCAACCGCCCACGCTCTGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCgg < 2:7329/132‑1 (MQ=255) aCATCCCCCATATCCCCACCCACGCCCGCACGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCtt < 3:230477/135‑1 (MQ=255) cACCCAGATGACCACCGAAGCGCGCAAGCTCGTCGGCGACTGGATCCGCCAAGGCGCCCCCCGGCACAGCCCCCACCTCCCCAGGCAACACCCCCCCCAAGGGCATCCACATCCCCTGGCGGATCGGGGTGGccc > 3:102761/1‑135 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGCACGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCACGCAACCCGCCCCCCAAGGGAATCACCTACCCCAGCAGGAGCGGGCGTGCCCGCGCAGAGGc > 1:105992/1‑135 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCACCTTCCCCGGCAGGAGCGGGCTTGCCCGCGAAGAGGc > 8:29741/1‑135 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATACCCAAAGGCGCCCACGTCAACTCACACCACCTCGCCAGGCCAACCCCCCCCCAAGGGAATCCACATACCCAGCAGGACCGGGCATTGCCGCGAAGAAGc > 4:108790/1‑135 (MQ=255) agagCGCAAGCTCGTCGGCGACTGGCTCGCCAAACGCGCCCAGGTCCACGGACAGCACCGCGCCCGGCCACCCCCCCCCCAAGGGCATCCCCTTCCCCCGCACGCGCGGGCTTGGCCGCGCAGCGGCCCCTAGcc > 6:218305/1‑135 (MQ=255) cacgggcgtcgGCGACTGGAGCCCCAACGGCGCCCAGGACAACTCACAGCACCTCCCCCGGCAACCCGCCCCCCCAGGGAAACACCTTCCCCGGCAGGCGCGGGCTTGCCCGCTACGAGGCCGATAGCCCCAGcc < 4:296793/129‑1 (MQ=255) gCGACTGGGTAGCCCAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCCACCCGCCCCCCAAGCGAAGCACCATCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGAAAGCCCCCTCCTCAAGCTCCaa > 2:31415/1‑134 (MQ=255) gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCCACCCGCCCCCCAAGGGCATCACCTTCCCCTGCAGGCGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCCGCCTCAAGCTCCAt > 3:93738/1‑135 (MQ=255) agACTGGAGCGCCAATGGCGGCACGGTCATATGAACGAACCGCCCCCGGCACCCCGCCCCCCTGGTGATTCAGGTGCCCCTGCTGGAGGGGGGTGCCGCGGTATGAGGACGATAGCCCCAGCCTCAAGCTCCAtg < 6:144699/134‑1 (MQ=255) gggttCGCCAATGGCGCCCTGGTCAACTGACCGACCCGCGCCAGCCACCCCGCCCCACATGGTAAACCCTTTCCCAGGCAGGCGCGGGCTTGCCGGCTATGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGtt < 4:62342/131‑1 (MQ=255) agcGCCAACGGCGCCCAGGTCACCTGACAGCACCGCCCCCGGCACCCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg < 8:291539/133‑1 (MQ=255) tCGCCAAAGGCGCCCAGGTCAACTGACAGCCCCTCGCCCGGCCACCCCCCCCCCCACGGAATCACCTTCCCCGGCCGGCGCGGGCGTGCCCGCGAAGACGCCGATAGCCCCCGCCGCAAGCTCCAAGTGTTaag > 4:148064/1‑132 (MQ=255) ccAACGCCGCCCTGGTAAACTTACCCCACCGCCCCAGGCAACCCGCCCCCCAAGGTAATCACCTTCCCCGGCAGGTGCGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac < 2:235767/135‑1 (MQ=255) aaaGGCGCCCAGGGAAACTTACACCACCTCGCCAGGCAACCCGCCCCCCAAGGGAAGCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaacaa < 4:172230/135‑1 (MQ=255) tCAACTGACAGCACCTCGCCAGGCACCCCGCCCCCCACGGGAATCCCCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 4:62795/135‑1 (MQ=255) tCAACTCCCAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAAGCACCTGCCCGTGCAGCAGCGGGCTGGCCCGCGACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 7:231880/135‑1 (MQ=255) caaaCTTACACCACCTCGCCCGGAAACCCCCCCCCCACGGAAACCACCTACCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 8:297669/133‑1 (MQ=255) aCAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAgg > 1:151811/1‑135 (MQ=255) aCCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAt > 6:104223/1‑133 (MQ=255) aCCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAt > 4:146986/1‑133 (MQ=255) aCCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAt > 3:121948/1‑133 (MQ=255) tccccAGGCAACCCGCCCCCCAAGGGAATAACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc < 4:245594/132‑1 (MQ=255) | GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGC > KT2440/4874436‑4874696 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |