Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,986,317 A→T 9.1% I150F (ATC→TTC)  bkdA1 → 2‑oxoisovalerate dehydrogenase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,986,3170AT 9.1% 114.8 / 4.4 44I150F (ATC→TTC) bkdA12‑oxoisovalerate dehydrogenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (20/20);  new base T (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

CTACATGCAAAGCCTGGGCGAAGAAGCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGCGACCCAGTTCGTGCAGGCGGTCGG  >  KT2440/4986184‑4986447
                                                                                                                                     |                                                                                                                                  
cTACATGCAAAGCCTGGGCGAAGAAGCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCTCTGGTCGAgatgat                                                                                                                                   >  2:203040/1‑135 (MQ=255)
        aaaGCCTGGGCGAAGAAGCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAAc                                                                                                                           >  5:49402/1‑135 (MQ=255)
         aaGCCTGGGCGAAGAAGCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAAct                                                                                                                          >  6:163444/1‑135 (MQ=255)
             cTGGGCGAAGAAGCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGCCCTCCCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAActgctg                                                                                                                      >  6:4478/1‑135 (MQ=255)
                ggCTAAGAAGCCATCGGCAGCGGCCTGGGGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCTACGTGTCGCTGGTCGAGATTATCTGCCAACTGCTGTcc                                                                                                                   <  6:287250/135‑1 (MQ=255)
                   gaagaaGCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAAc                                                                                                                >  8:36943/1‑135 (MQ=255)
                   gaagaaGCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAAc                                                                                                                >  5:234578/1‑135 (MQ=255)
                        agCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAgcg                                                                                                           <  5:97103/135‑1 (MQ=255)
                        agCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAgcg                                                                                                           >  5:104736/1‑135 (MQ=255)
                         gCCATCGGCAGCGGCCAGGCGCTGGCGCTGCACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAgcgc                                                                                                          >  8:197077/1‑135 (MQ=255)
                         gCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGTTCTGCCAACTGCTGTCCAACGAgcgc                                                                                                          >  6:75452/1‑135 (MQ=255)
                         gCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGTTCTGCCAACTGCTGTCCAACGAgcgc                                                                                                          >  6:71197/1‑135 (MQ=255)
                         gCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAgcgc                                                                                                          >  5:149436/1‑135 (MQ=255)
                         gCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAgcgc                                                                                                          >  5:248143/1‑135 (MQ=255)
                         gCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGGCGAGATGATCTGCCAACTGCTGTCCAACGAgcgc                                                                                                          <  6:261914/135‑1 (MQ=255)
                         gCCAGCGGCAGCGGCCAGGCGCTGGCGCTGTAGCGCACCGACTTGTGCTTCCCGAACTACCGCCAGCAAAGCATCCTGATGGCCCGCGACTTGTCGCTGGTCTAGATGATATGCCAACTTCTTTCCAACGAgcgc                                                                                                          <  8:137352/135‑1 (MQ=255)
                             tCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGAcc                                                                                                      >  5:268883/1‑135 (MQ=255)
                                gaggCGGCCAGGCGGGGGCGCTGAACCCCACCTACATGTGCTTCCAGACCTACCGCCACCAAAGCAGCCTGAGGGCCCGCGACGTGTCGCTGGTCTATATGATCTGCCAACTGCTGGCAAACGAGCGCGAccccc                                                                                                   <  6:191071/132‑1 (MQ=255)
                                gCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGTTCTGCCAACTGCTGTCCAACGAGCGCGAccccc                                                                                                   <  5:71197/135‑1 (MQ=255)
                                gCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGTTCTGCCAACTGCTGTCCAACGAGCGCGAccccc                                                                                                   <  5:75452/135‑1 (MQ=255)
                                    cGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCaa                                                                                               <  2:172925/135‑1 (MQ=255)
                                    cGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCaa                                                                                               >  3:165238/1‑135 (MQ=255)
                                            cgctggcgctgAACCGCACCGACATGGGCTACCCGACCAACCGCCACCAAACCAGCCTGATGGCCCGCGACGTGGCGGTGGGCGAGATGATCTGCCAACTGCTGTCCACCGAGCGCGACCCCCTCAAGGgccgcc                                                                                       <  5:174717/135‑1 (MQ=255)
                                               tggcgctgAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGt                                                                                    <  6:115446/135‑1 (MQ=255)
                                                 gcgctgAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTg                                                                                  <  7:84157/135‑1 (MQ=255)
                                                   gctgAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGCCGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCACGGGCAGCCAGTTGcc                                                                                >  3:65391/1‑135 (MQ=255)
                                                      gAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCACCTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGt                                                                                    >  1:195951/1‑128 (MQ=255)
                                                      gAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGGCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGt                                                                                    <  2:195951/128‑1 (MQ=255)
                                                         ccGCCCCGACATGTGCGTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGCGATGATCTGCCACCTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCAt                                                                          >  5:209562/1‑135 (MQ=255)
                                                         ccGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCAt                                                                          <  5:120256/135‑1 (MQ=255)
                                                             aCCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTAt                                                                      <  6:268837/135‑1 (MQ=255)
                                                                       gCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCa                                                                                                                             >  6:175784/1‑70 (MQ=255)
                                                                       gCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCa                                                                                                                             <  5:175784/70‑1 (MQ=255)
                                                                                 cTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGGCGAGATGATCTGCCAACTGCGGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGctt                                                  <  7:197077/135‑1 (MQ=255)
                                                                                        cAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCCGTTGCCGCACATGTATTCGGTGCGCGAAGCCGGgttcggccc                                            >  5:190023/1‑129 (MQ=255)
                                                                                                    cTGATGGCCCGCGACGTGGCGCGGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAAc                               <  7:223004/135‑1 (MQ=255)
                                                                                                           cccGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGCg                         <  8:218963/134‑1 (MQ=255)
                                                                                                           cccGCGACGGGGCGCGGGTCGAGATGATCTGCCAACTGCTGTCCACCGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGc                          <  5:275372/133‑1 (MQ=255)
                                                                                                                  cGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGCTCATGTATTCGGTGCGCGAAGCCGGCGTCTTCACCATCAGCGGCACCCTGGCGACCCAGtt                 >  1:38137/1‑135 (MQ=255)
                                                                                                                    tgtCGCTGGTCGAGATGATCTGCAAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGCGACCCAGTTCg               <  3:287965/135‑1 (MQ=255)
                                                                                                                       cGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATGATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGCGACCCAGTTCGTGc            <  4:165238/135‑1 (MQ=255)
                                                                                                                       cGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGCGACCCAGTTCGt              >  6:26246/1‑133 (MQ=255)
                                                                                                                        gCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCACCCTGGCGCCCCAGTTCGTGCa           >  2:261701/1‑135 (MQ=255)
                                                                                                                               gagaTGATCTGCCAACTGCTGTCCAACTAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGCGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGc                          <  5:281/113‑1 (MQ=255)
                                                                                                                               gagaTGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGc                          >  6:281/1‑113 (MQ=255)
                                                                                                                                 gatgatCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGCGACCCAGTTCGTGCAGGCGGTCgg  >  3:238929/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                  
CTACATGCAAAGCCTGGGCGAAGAAGCCATCGGCAGCGGCCAGGCGCTGGCGCTGAACCGCACCGACATGTGCTTCCCGACCTACCGCCAGCAAAGCATCCTGATGGCCCGCGACGTGTCGCTGGTCGAGATGATCTGCCAACTGCTGTCCAACGAGCGCGACCCCCTCAAGGGCCGCCAGTTGCCGATCATGTATTCGGTGCGCGAAGCCGGCTTCTTCACCATCAGCGGCAACCTGGCGACCCAGTTCGTGCAGGCGGTCGG  >  KT2440/4986184‑4986447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑