Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,336,288	G→A	7.0%	D258N (GAT→AAT)	panC →	Pantothenate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,336,288	0	G	A	7.0%	178.1 / 2.3	58	D258N (GAT→AAT)	panC	Pantothenate synthetase
Reads supporting (aligned to +/- strand):  ref base G (24/28);  new base A (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCATCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAA  >  KT2440/5336155‑5336416                                                                                                                                      |                                                                                                                                 gggCATCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGa                                                                                                                                 <  1:141858/135‑1 (MQ=255) gggCATCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGa                                                                                                                                 <  6:46980/135‑1 (MQ=255)  ggCATCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGAt                                                                                                                                <  6:90710/135‑1 (MQ=255)   gCATCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGAt                                                                                                                                >  5:218948/1‑134 (MQ=255)   gCATCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGAt                                                                                                                                >  7:233244/1‑134 (MQ=255)     aTCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGAt                                                                                                                                >  1:190253/1‑132 (MQ=255)     aTCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCga                                                                                                                             >  7:1630/1‑135 (MQ=255)     aTCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCga                                                                                                                             >  5:270898/1‑135 (MQ=255)     aTCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGAATACCTGGAAGTACGCCATGCCGAAAGCTTCCGCCCGGCAGTGATCAATGAt                                                                                                                                >  7:66499/1‑132 (MQ=255)     aTCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGGCGCTGAAGGCCAGGCGCAACTGAGCGCCGCGGGTTTCCGCCCGGATTACCTGGCAGTACTCCATGCCGTAAGCTTGCGCCCGGCAGTGCTCAATGATCga                                                                                                                             >  2:291317/1‑135 (MQ=255)         gCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGAc                                                                                                                          <  7:182922/134‑1 (MQ=255)                    cGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGAtt                                                                                                              <  7:63090/135‑1 (MQ=255)                       gATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATAATCGAGACCTGGTGGTGATTgcg                                                                                                           >  4:138063/1‑135 (MQ=255)                       gATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATAATCGAGACCTGGTGGTGATTgcg                                                                                                           >  4:133199/1‑135 (MQ=255)                              ccGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCt                                                                                                     <  4:2265/134‑1 (MQ=255)                                    tGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTgg                                                                                              <  5:254911/135‑1 (MQ=255)                                          cTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAaca                                                                                        <  5:240252/135‑1 (MQ=255)                                               ggCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCgg                                                                                   <  6:270898/135‑1 (MQ=255)                                                   aGGCGCAACAGAGCGCCGCGGGTTTCCGCCCGGATTAACTGGAAGTACGCCATGCCGTAAGCTTTCGCCCGGCAGTGATAAATTATAGAGACCTGGTGGTGATTGCGGCGGCGTACCTGGGGAACACGCGGGTgc                                                                               >  4:164581/1‑134 (MQ=255)                                                    ggCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGAt                                                                              <  3:78772/135‑1 (MQ=255)                                                    ggCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGAt                                                                              <  7:273085/135‑1 (MQ=255)                                                        cAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGa                                                                           >  5:74531/1‑134 (MQ=255)                                                        cAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGAc                                                                          <  4:88563/135‑1 (MQ=255)                                                        cAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGGTGATCGa                                                                           >  5:168953/1‑134 (MQ=255)                                                              aGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACCACctc                                                                    >  1:79873/1‑135 (MQ=255)                                                               gcgcAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACctc                                                                    <  3:213608/134‑1 (MQ=255)                                                                 gcAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTAc                                                                 >  8:97346/1‑135 (MQ=255)                                                                 gcAGAGGGTTTCCGCCCGGATTACCTTGAAGTACGCCATGCCGAAAGCTTGCGCCCGGCAGTGATAAATGATCGAGACCTGGTTGTGAATGCGGCGGCATACCTGGGGAACACGCGGTTGCTCGAAAACCTCTAc                                                                 >  7:126549/1‑135 (MQ=255)                                                                   aGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTTATTGCGGCGGCTTACCTGGGTAACACGCGGATGATCGACAACCTCTACCt                                                               >  2:8893/1‑135 (MQ=255)                                                                   aGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCt                                                               >  3:224560/1‑135 (MQ=255)                                                                   aGCGGGTTTCCGCCCGGATTAACTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGATGATAGACAACCTCTACCt                                                               >  5:59560/1‑135 (MQ=255)                                                                       ggTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCAt                                                           <  8:220228/135‑1 (MQ=255)                                                                               cccGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATAATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGgaaga                                                   <  3:133199/135‑1 (MQ=255)                                                                               cacGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATAATCGAGACATGGTGGGTATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTTCATTTGgaaga                                                   <  3:138063/133‑1 (MQ=255)                                                                                 gggATTACCTGGAAGTACGCCATGCGTTATGGTTGGGCCGGGCTGTTATAATTTTTCGAGACGTGGTGGTTTTTGCGGCGGATTACCTGGTTAACACGCTGTTTAGCGACAACCTATACATGCATTTGgaagaga                                                 <  6:176437/134‑1 (MQ=255)                                                                                 cGGATTACCTGGAAGTACGCCATGCCGTAAGATTGGGCGCGGCAGTGATCAATGATCGAGACGTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTTATCGACAACCTCTACCTGCTTTTGtaagaga                                                 <  3:226820/135‑1 (MQ=255)                                                                                       aCCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACATGGGTATCACTCGGTTGATCGCCACCCTCTAACTGCATTTGGAAGAGAAGAcc                                            >  6:200249/1‑134 (MQ=255)                                                                                         cTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCa                                         <  8:247865/135‑1 (MQ=255)                                                                                         cTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCa                                         <  8:23837/135‑1 (MQ=255)                                                                                         cTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCa                                         <  8:270791/135‑1 (MQ=255)                                                                                         cTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGAAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCa                                         <  4:124201/135‑1 (MQ=255)                                                                                          tGGAAGTACGCCATGCCGTAAGCTTGCTCCCGGCAGTGATGAATGATCGAGTCCTGGTGGTTATTGCTGCTGCTTACCTTGGTAAATCGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCa                                         <  1:8893/134‑1 (MQ=255)                                                                                           ggAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTAATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATa                                       >  2:187310/1‑135 (MQ=255)                                                                                                 aCGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTc                                 >  4:111325/1‑135 (MQ=255)                                                                                                     cATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCt                                                                                                     >  2:89597/1‑63 (MQ=255)                                                                                                     cATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCt                                                                                                     <  1:89597/63‑1 (MQ=255)                                                                                                     cATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGgaagaga                                                 >  6:27974/1‑115 (MQ=255)                                                                                                     cATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGgaagaga                                                 <  5:27974/115‑1 (MQ=255)                                                                                                     cATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTgg                             >  8:63941/1‑135 (MQ=255)                                                                                                     cATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTgg                             >  4:64900/1‑135 (MQ=255)                                                                                                           gTAAGCTTGCGCCCGGCAGGGATCAATGAGCGAGACCTGGTGGGGATGGCGGCGGCTTACCTGGGTAACACGCGGGTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGcc                       <  7:287490/135‑1 (MQ=255)                                                                                                                       ccGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGGTGGCGTGGAGccccat           >  4:125134/1‑132 (MQ=255)                                                                                                                          gCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTcgc        <  8:233244/135‑1 (MQ=255)                                                                                                                          gCAGTGATCAATGAGCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGTAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTcgc        <  6:218948/135‑1 (MQ=255)                                                                                                                             gTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTa                                                                  >  4:28576/1‑74 (MQ=255)                                                                                                                             gTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTa                                                                  <  3:28576/74‑1 (MQ=255)                                                                                                                                 tCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCaa  <  7:63941/134‑1 (MQ=255)                                                                                                                                 tCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTCATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCaa  <  1:187310/134‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 GGGCATCGGCCGTGGCCAGCGGGATTTTGCCGCGCTGGTCGCTGAAGGCCAGGCGCAACTGAGCGCAGCGGGTTTCCGCCCGGATTACCTGGAAGTACGCCATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAA  >  KT2440/5336155‑5336416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑