Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,624,908 C→T 12.8% F203F (TTC→TTT)  putA → Bifunctional protein PutA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,624,9080CT12.8% 105.6 / 7.3 39F203F (TTC→TTT) putABifunctional protein PutA
Reads supporting (aligned to +/- strand):  ref base C (20/14);  new base T (2/3);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

GGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCG  >  KT2440/5624782‑5625038
                                                                                                                              |                                                                                                                                  
ggCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAAcgc                                                                                                                            >  1:197902/1‑135 (MQ=255)
ggCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAAcgc                                                                                                                            >  4:67320/1‑135 (MQ=255)
     tGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCa                                                                                                                       <  4:267678/135‑1 (MQ=255)
          tgCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTgg                                                                                                                  >  3:28275/1‑135 (MQ=255)
                 cggAAGCGTTGTTGCGTATTCCCGACAAAGCCACCCGCGACGCGTTAATTCGCGACAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGTAGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGgctgc                                                                                                           >  8:95645/3‑135 (MQ=255)
                  cGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTc                                                                                                                                    <  8:267616/109‑1 (MQ=255)
                  cGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTc                                                                                                                                    >  7:267616/1‑109 (MQ=255)
                        ccTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGAc                                                                                                    >  6:73547/1‑135 (MQ=255)
                            ctgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGc                                                                                                <  8:203806/135‑1 (MQ=255)
                             tgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGc                                                                                                <  3:67320/134‑1 (MQ=255)
                                    ccccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGt                                                                                        <  6:211271/135‑1 (MQ=255)
                                    ccccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTCGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTgg                                                                                         >  5:125754/1‑134 (MQ=255)
                                       cGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGAACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCAATGTTCGTCAACGCCGCCACCTGTGCCCTGCTGCTGCACGGCAAGCTGCTCAg                                                                                     >  7:12614/1‑135 (MQ=255)
                                                  cccGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACg                                                                          <  4:199381/135‑1 (MQ=255)
                                                     gTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGaaa                                                                       >  7:217478/1‑135 (MQ=255)
                                                       gATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGTCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAAcc                                                                     >  8:116209/1‑135 (MQ=255)
                                                            gcTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGcc                                                                 >  2:181822/1‑134 (MQ=255)
                                                              tGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCa                                                              >  4:265839/1‑135 (MQ=255)
                                                                   cgcgACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCCTAACGAAACCGGCCTCAcctcc                                                         >  1:135487/1‑135 (MQ=255)
                                                                   cgcgACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctcc                                                         >  8:267732/1‑135 (MQ=255)
                                                                     cgACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACctcctc                                                       <  3:275241/135‑1 (MQ=255)
                                                                       aCAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGc                                                     <  6:7225/135‑1 (MQ=255)
                                                                        cAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGTAGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACg                                                                          <  5:265375/113‑1 (MQ=255)
                                                                        cAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGTAGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACg                                                                          >  6:265375/1‑113 (MQ=255)
                                                                             tCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCAccc                                               <  8:110751/135‑1 (MQ=255)
                                                                                   ccGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGcatca                                         >  7:183757/1‑135 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctcc                                                         <  8:257289/113‑1 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctcc                                                         >  7:257289/1‑113 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCCTCGGCa                                   >  3:166808/1‑135 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCgg                                     >  1:181731/1‑133 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCgg                                     >  2:170548/1‑133 (MQ=255)
                                                                                            ggCAGCCGCTCCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTCGTCAGCACCCATAACGCAACCGGCCTCACCACCTCGCTCACCCGCATCATCGGCAaga                                >  6:267141/1‑135 (MQ=255)
                                                                                                     aCCTGGGCAACAGCCCGTCACTGTTCCTCTCCGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCGGGTCAGCACCCAAAACGAACCCGGCCTCACCTCCACGCTCACCCGCATCATCGGCAAGCGCGGCCAGc                       >  4:162553/1‑135 (MQ=255)
                                                                                                             cgtaGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACGAAGCCAGCCTCTCCCGCTCGCTGAACCGCATTATCGGTAAAAGCGGTGAACCGCTGATc               <  1:290358/132‑1 (MQ=255)
                                                                                                              aCAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATcc              <  7:51485/135‑1 (MQ=255)
                                                                                                              aCAGCCCGTCACTGTTCGGCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATcc              <  1:68486/135‑1 (MQ=255)
                                                                                                                  cccGTCACTGGTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCaa          <  2:197902/135‑1 (MQ=255)
                                                                                                                   ccGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACGAAGCCAGCCTCTCCCGCTCGCTGAACCGCATTATCGGTAAAAGCGGTGAACCGCTGATCCGCaaa         <  7:95645/135‑2 (MQ=255)
                                                                                                                    cGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCCGCGAGCCGATGATCCGCAAgg        >  4:275137/1‑135 (MQ=255)
                                                                                                                     gTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAggg       <  6:125754/135‑1 (MQ=255)
                                                                                                                          tGTTCTTAAACGGCGCGACCTGGGTTCTGCTTCTGTCCGGCAAGCTTGTCAGCACCCATAACGAAACCGGCGTCAGCTCTTCGCTCACCCGCATCATCTTCAAGAGCGGCGAGCCGATGATCCGCAAGGGcttag  <  6:131531/135‑5 (MQ=255)
                                                                                                                              |                                                                                                                                  
GGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCG  >  KT2440/5624782‑5625038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑