Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,624,911	C→T	12.0%	V204V (GTC→GTT)	putA →	Bifunctional protein PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,624,911	0	C	T	12.0%	117.2 / 6.6	42	V204V (GTC→GTT)	putA	Bifunctional protein PutA
Reads supporting (aligned to +/- strand):  ref base C (22/14);  new base T (2/3);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGG  >  KT2440/5624782‑5625044                                                                                                                                  |                                                                                                                                      ggCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAAcgc                                                                                                                                  >  4:67320/1‑135 (MQ=255) ggCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAAcgc                                                                                                                                  >  1:197902/1‑135 (MQ=255)      tGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCa                                                                                                                             <  4:267678/135‑1 (MQ=255)           tgCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTgg                                                                                                                        >  3:28275/1‑135 (MQ=255)                  cggAAGCGTTGTTGCGTATTCCCGACAAAGCCACCCGCGACGCGTTAATTCGCGACAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGTAGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGgctgc                                                                                                                 >  8:95645/3‑135 (MQ=255)                         ccTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGAc                                                                                                          >  6:73547/1‑135 (MQ=255)                             ctgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGc                                                                                                      <  8:203806/135‑1 (MQ=255)                              tgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGc                                                                                                      <  3:67320/134‑1 (MQ=255)                                     ccccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGt                                                                                              <  6:211271/135‑1 (MQ=255)                                     ccccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTCGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTgg                                                                                               >  5:125754/1‑134 (MQ=255)                                        cGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGAACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCAATGTTCGTCAACGCCGCCACCTGTGCCCTGCTGCTGCACGGCAAGCTGCTCAg                                                                                           >  7:12614/1‑135 (MQ=255)                                                   cccGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACg                                                                                <  4:199381/135‑1 (MQ=255)                                                      gTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGaaa                                                                             >  7:217478/1‑135 (MQ=255)                                                        gATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGTCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAAcc                                                                           >  8:116209/1‑135 (MQ=255)                                                             gcTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGcc                                                                       >  2:181822/1‑134 (MQ=255)                                                               tGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCa                                                                    >  4:265839/1‑135 (MQ=255)                                                                    cgcgACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCCTAACGAAACCGGCCTCAcctcc                                                               >  1:135487/1‑135 (MQ=255)                                                                    cgcgACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctcc                                                               >  8:267732/1‑135 (MQ=255)                                                                      cgACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACctcctc                                                             <  3:275241/135‑1 (MQ=255)                                                                        aCAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGc                                                           <  6:7225/135‑1 (MQ=255)                                                                         cAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGTAGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACg                                                                                >  6:265375/1‑113 (MQ=255)                                                                         cAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGTAGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACg                                                                                <  5:265375/113‑1 (MQ=255)                                                                              tCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCAccc                                                     <  8:110751/135‑1 (MQ=255)                                                                                    ccGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGcatca                                               >  7:183757/1‑135 (MQ=255)                                                                                          aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctcc                                                               <  8:257289/113‑1 (MQ=255)                                                                                          aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctcc                                                               >  7:257289/1‑113 (MQ=255)                                                                                          aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCCTCGGCa                                         >  3:166808/1‑135 (MQ=255)                                                                                          aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCgg                                           >  1:181731/1‑133 (MQ=255)                                                                                          aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCgg                                           >  2:170548/1‑133 (MQ=255)                                                                                             ggCAGCCGCTCCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTCGTCAGCACCCATAACGCAACCGGCCTCACCACCTCGCTCACCCGCATCATCGGCAaga                                      >  6:267141/1‑135 (MQ=255)                                                                                                      aCCTGGGCAACAGCCCGTCACTGTTCCTCTCCGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCGGGTCAGCACCCAAAACGAACCCGGCCTCACCTCCACGCTCACCCGCATCATCGGCAAGCGCGGCCAGc                             >  4:162553/1‑135 (MQ=255)                                                                                                              cgtaGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACGAAGCCAGCCTCTCCCGCTCGCTGAACCGCATTATCGGTAAAAGCGGTGAACCGCTGATc                     <  1:290358/132‑1 (MQ=255)                                                                                                               aCAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATcc                    <  7:51485/135‑1 (MQ=255)                                                                                                               aCAGCCCGTCACTGTTCGGCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATcc                    <  1:68486/135‑1 (MQ=255)                                                                                                                   cccGTCACTGGTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCaa                <  2:197902/135‑1 (MQ=255)                                                                                                                    ccGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACGAAGCCAGCCTCTCCCGCTCGCTGAACCGCATTATCGGTAAAAGCGGTGAACCGCTGATCCGCaaa               <  7:95645/135‑2 (MQ=255)                                                                                                                     cGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCCGCGAGCCGATGATCCGCAAgg              >  4:275137/1‑135 (MQ=255)                                                                                                                      gTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAggg             <  6:125754/135‑1 (MQ=255)                                                                                                                           tGTTCTTAAACGGCGCGACCTGGGTTCTGCTTCTGTCCGGCAAGCTTGTCAGCACCCATAACGAAACCGGCGTCAGCTCTTCGCTCACCCGCATCATCTTCAAGAGCGGCGAGCCGATGATCCGCAAGGGcttag        <  6:131531/135‑5 (MQ=255)                                                                                                                                gTCAACGCCCCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCCCCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCCCAAGGGCGTCGCCaag   >  5:72776/1‑133 (MQ=255)                                                                                                                                 tCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATgg  <  2:181731/135‑1 (MQ=255)                                                                                                                                 tCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATgg  >  4:233976/1‑135 (MQ=255)                                                                                                                                  |                                                                                                                                      GGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGG  >  KT2440/5624782‑5625044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑