Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,624,914 C→T 12.2% N205N (AAC→AAT)  putA → Bifunctional protein PutA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,624,9140CT12.2% 113.7 / 6.7 41N205N (AAC→AAT) putABifunctional protein PutA
Reads supporting (aligned to +/- strand):  ref base C (20/16);  new base T (2/3);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCC  >  KT2440/5624782‑5625046
                                                                                                                                    |                                                                                                                                    
ggCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAAcgc                                                                                                                                    >  1:197902/1‑135 (MQ=255)
ggCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAAcgc                                                                                                                                    >  4:67320/1‑135 (MQ=255)
     tGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCa                                                                                                                               <  4:267678/135‑1 (MQ=255)
          tgCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTgg                                                                                                                          >  3:28275/1‑135 (MQ=255)
                 cggAAGCGTTGTTGCGTATTCCCGACAAAGCCACCCGCGACGCGTTAATTCGCGACAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGTAGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGgctgc                                                                                                                   >  8:95645/3‑135 (MQ=255)
                        ccTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGAc                                                                                                            >  6:73547/1‑135 (MQ=255)
                            ctgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGc                                                                                                        <  8:203806/135‑1 (MQ=255)
                             tgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGc                                                                                                        <  3:67320/134‑1 (MQ=255)
                                    ccccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGt                                                                                                <  6:211271/135‑1 (MQ=255)
                                    ccccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTCGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTgg                                                                                                 >  5:125754/1‑134 (MQ=255)
                                       cGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGAACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCAATGTTCGTCAACGCCGCCACCTGTGCCCTGCTGCTGCACGGCAAGCTGCTCAg                                                                                             >  7:12614/1‑135 (MQ=255)
                                                  cccGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACg                                                                                  <  4:199381/135‑1 (MQ=255)
                                                     gTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGaaa                                                                               >  7:217478/1‑135 (MQ=255)
                                                       gATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGTCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAAcc                                                                             >  8:116209/1‑135 (MQ=255)
                                                            gcTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGcc                                                                         >  2:181822/1‑134 (MQ=255)
                                                              tGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCa                                                                      >  4:265839/1‑135 (MQ=255)
                                                                   cgcgACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCCTAACGAAACCGGCCTCAcctcc                                                                 >  1:135487/1‑135 (MQ=255)
                                                                   cgcgACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctcc                                                                 >  8:267732/1‑135 (MQ=255)
                                                                     cgACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACctcctc                                                               <  3:275241/135‑1 (MQ=255)
                                                                       aCAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGc                                                             <  6:7225/135‑1 (MQ=255)
                                                                        cAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGTAGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACg                                                                                  <  5:265375/113‑1 (MQ=255)
                                                                        cAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGTAGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACg                                                                                  >  6:265375/1‑113 (MQ=255)
                                                                             tCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCAccc                                                       <  8:110751/135‑1 (MQ=255)
                                                                                   ccGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGcatca                                                 >  7:183757/1‑135 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctcc                                                                 <  8:257289/113‑1 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctcc                                                                 >  7:257289/1‑113 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCCTCGGCa                                           >  3:166808/1‑135 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCgg                                             >  1:181731/1‑133 (MQ=255)
                                                                                         aCTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCgg                                             >  2:170548/1‑133 (MQ=255)
                                                                                            ggCAGCCGCTCCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTCGTCAGCACCCATAACGCAACCGGCCTCACCACCTCGCTCACCCGCATCATCGGCAaga                                        >  6:267141/1‑135 (MQ=255)
                                                                                                     aCCTGGGCAACAGCCCGTCACTGTTCCTCTCCGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCGGGTCAGCACCCAAAACGAACCCGGCCTCACCTCCACGCTCACCCGCATCATCGGCAAGCGCGGCCAGc                               >  4:162553/1‑135 (MQ=255)
                                                                                                             cgtaGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACGAAGCCAGCCTCTCCCGCTCGCTGAACCGCATTATCGGTAAAAGCGGTGAACCGCTGATc                       <  1:290358/132‑1 (MQ=255)
                                                                                                              aCAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATcc                      <  7:51485/135‑1 (MQ=255)
                                                                                                              aCAGCCCGTCACTGTTCGGCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATcc                      <  1:68486/135‑1 (MQ=255)
                                                                                                                  cccGTCACTGGTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCaa                  <  2:197902/135‑1 (MQ=255)
                                                                                                                   ccGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTTTCCACCCATAACGAAGCCAGCCTCTCCCGCTCGCTGAACCGCATTATCGGTAAAAGCGGTGAACCGCTGATCCGCaaa                 <  7:95645/135‑2 (MQ=255)
                                                                                                                    cGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCCGCGAGCCGATGATCCGCAAgg                >  4:275137/1‑135 (MQ=255)
                                                                                                                     gTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAggg               <  6:125754/135‑1 (MQ=255)
                                                                                                                          tGTTCTTAAACGGCGCGACCTGGGTTCTGCTTCTGTCCGGCAAGCTTGTCAGCACCCATAACGAAACCGGCGTCAGCTCTTCGCTCACCCGCATCATCTTCAAGAGCGGCGAGCCGATGATCCGCAAGGGcttag          <  6:131531/135‑5 (MQ=255)
                                                                                                                               gTCAACGCCCCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCCCCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCCCAAGGGCGTCGCCaag     >  5:72776/1‑133 (MQ=255)
                                                                                                                                tCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATgg    <  2:181731/135‑1 (MQ=255)
                                                                                                                                tCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATgg    >  4:233976/1‑135 (MQ=255)
                                                                                                                                   aCGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGcc  <  1:170548/134‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GGCGCTGATGTGCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCC  >  KT2440/5624782‑5625046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑