Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	515,063	G→A	45.2%	D236D (GAC→GAT)	mepM ←	Murein DD‑endopeptidase MepM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	515,063	0	G	A	45.2%	8.0 / 53.6	42	D236D (GAC→GAT)	mepM	Murein DD‑endopeptidase MepM
Reads supporting (aligned to +/- strand):  ref base G (11/12);  new base A (6/13);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.25e-01

AGGTTTTGCCGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGCTGATT  >  KT2440/514933‑515192                                                                                                                                   |                                                                                                                                  aGGTTTTGCCGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCg                                                                                                                                >  4:186747/1‑134 (MQ=255)        gCCGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCa                                                                                                                         >  3:175619/1‑134 (MQ=255)        gCCGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAg                                                                                                                        >  1:75971/1‑135 (MQ=255)         ccGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAg                                                                                                                        <  7:59323/134‑1 (MQ=255)           gcgGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCAc                                                                                                                     >  6:197829/1‑135 (MQ=255)              gTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCAcgcg                                                                                                                  <  5:219855/135‑1 (MQ=255)               ttGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCgg                                                                                                                 <  1:122145/135‑1 (MQ=255)               ttGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCgg                                                                                                                 <  4:175619/135‑1 (MQ=255)               gtGGGGAAGCGCGAAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGTACTCGTCCCCAGTGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCgg                                                                                                                 <  6:152623/134‑1 (MQ=255)                tGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCggg                                                                                                                <  7:110573/135‑1 (MQ=255)                 ggTGAAGCGCGCAGATAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACACCGCACGCGGGc                                                                                                               >  8:142741/1‑135 (MQ=255)                                    aTGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCtg                                                                                            >  5:208349/1‑135 (MQ=255)                                            ggTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTATACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAg                                                                                    >  1:215589/1‑135 (MQ=255)                                            ggTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAg                                                                                    >  1:230506/1‑135 (MQ=255)                                            ggTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAg                                                                                    >  3:193856/1‑135 (MQ=255)                                                ccGACGACCTTGCCATCCATCACCTTCTGTTCGTAGCCCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCCGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACCGGCCg                                                                                >  6:199712/1‑135 (MQ=255)                                                  gacgacCTTGCCATCCATCTCCTTCTGTTAGTAGACCACGTCGAACTCGTCCCCCGGTCGAATATCATGTGCGACGTCTATATCGTCACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGgc                                                                              <  8:173226/135‑1 (MQ=255)                                                       ccTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCt                                                                         <  7:293419/135‑1 (MQ=255)                                                             catccatcACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCgg                                                                   <  4:145818/135‑1 (MQ=255)                                                             catccatcACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCg                                                                    >  4:47556/1‑134 (MQ=255)                                                                ccatcACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAg                                                                <  7:204685/135‑1 (MQ=255)                                                                  atcACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCGGTGGGACAGGCCGGCGCGCTGGGCGGAAGc                                                               <  8:30887/134‑1 (MQ=255)                                                                      ccTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACa                                                          <  5:139025/135‑1 (MQ=255)                                                                      ccTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACa                                                          <  7:53222/135‑1 (MQ=255)                                                                           tGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGa                                                      <  7:27322/134‑1 (MQ=255)                                                                           tGTTCGTAGACCACGACGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGGGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGa                                                      <  6:97633/134‑1 (MQ=255)                                                                            gTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGCAGCAGACAGCGAGc                                                    >  7:60647/1‑135 (MQ=255)                                                                            gTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGc                                                    >  3:122341/1‑135 (MQ=255)                                                                                      cACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAc                                          <  8:197890/135‑1 (MQ=255)                                                                                       ccGTCGACCTCTTCCCCAGGTCGAATATCCTGTGCGACGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCaac                                         <  8:34353/134‑3 (MQ=255)                                                                                       aCGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         <  7:228598/135‑1 (MQ=255)                                                                                       aCGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCAGGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         <  7:261280/135‑1 (MQ=255)                                                                                       aCGGCGACCTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGCCCATGGTCATGCTGTGGGACTGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         <  2:115996/135‑1 (MQ=255)                                                                                               cTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTggg                                  <  2:215589/134‑1 (MQ=255)                                                                                                      ccAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATgg                                                                                                    <  3:123784/61‑1 (MQ=255)                                                                                                      ccAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATgg                                                                                                    >  4:123784/1‑61 (MQ=255)                                                                                                               atatCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCTGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcccc                                       <  1:264753/113‑1 (MQ=255)                                                                                                               atatCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcccc                                       >  2:264753/1‑113 (MQ=255)                                                                                                                       tgCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGTGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         <  1:299583/103‑1 (MQ=255)                                                                                                                       tgCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGTGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         >  2:299583/1‑103 (MQ=255)                                                                                                                        gCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTg        >  6:155037/1‑135 (MQ=255)                                                                                                                              tCGATGTCGTAACCCAGCACGCGGGACATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGCTGAtt  <  4:74225/135‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  AGGTTTTGCCGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGCTGATT  >  KT2440/514933‑515192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑