| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,689,816 | A→C | 51.5% | S236R (AGC→CGC) | pyrC' → | Dihydroorotase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,689,816 | 0 | A | C | 51.5% | ‑4.2 / 8.9 | 21 | S236R (AGC→CGC) | pyrC' | Dihydroorotase‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (6/4); new base C (2/9); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01 | |||||||||||
AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCA > KT2440/5689692‑5689946 | aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCGTCAGCCCGCTCCCCCCCGccccggg > 4:99685/1‑131 (MQ=255) ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGGGGCCTTAAGCCCGATCACCACCCCCCCGGgcgcgc > 8:269758/1‑135 (MQ=255) ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGGGCACATGACCCCGATCACCAACGCCCCTGgcgcgc > 3:139334/1‑135 (MQ=255) ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGGCGACATCAGCCCACTCACCACCCCCCCTGgcgcgc > 1:50204/1‑135 (MQ=255) cgCTGCCGCGGCCGGCCGGGCATCCCGGAGCGCGCCGAAACGGTGGCCCTGGCGCGCAACCTGCGGCTGGGGGAACAGCGCGGCGGGCGTGCGCACTTCACCCAGATGACCAGCGCCCGGGGCGCGCGGCTGATc < 7:2449/134‑1 (MQ=255) cccGGCCTGCCGGGCACCCCGTACCGCGCCGAAACCGGGCCCCTCGCGCGCAACCTGCGGCTGGGGCACCAGCGGGGCGGGCGGGCGCAGTCCCCCCAGAACACCCGCGCCCGGGGCGCGCGGCTGATCgcccag < 2:170727/133‑1 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCCCGTCCGCCACCTCACCCCCGCCCCTGGCGCGCCGCGGCTCGCCCCCGCCCCCGCCGTGGGGCGGCCCGGAAccgc > 8:33862/1‑135 (MQ=255) aCCGGGGCCCGGGCGCGCAACCTGCTGCTGGTGCAACAGAGCGGCGTGCGTGCGCACTCCACCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg < 7:269758/133‑1 (MQ=255) ggggCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:73870/133‑1 (MQ=255) gTGGCCCTGGCGCGCAACATGCTGCTGGTGGAACAGCGCGGCGGGGGGGCGAACTTCAGCCAGAACCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 2:50204/135‑1 (MQ=255) gTGGCCCTGCCGCGCAACCGGCTGCCGGGGCCACAGAGCGGCGGGCGTGCGCACTTAAGCCATACCACCAGCGCCGGTGGCGCGCGTCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 1:20619/135‑1 (MQ=255) cgtGGGGCGCACCCTGGGGGTGGTGCACCCGCGCGGGGGGCGGGCGCACTGCCCCCAGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt < 1:235883/133‑1 (MQ=255) tgtaaCAGCGCGGGGGGCTGGCGCACTTCCGCCTGATCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACg < 7:227820/132‑1 (MQ=255) cggcggGCGTGCGCACTGCAGCCAGAACCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 4:259153/135‑1 (MQ=255) tgcgtgcgCACTTCACCCATAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 4:46421/135‑1 (MQ=255) tgcgggcgCACTTCCCCCAGAACACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 2:11372/135‑1 (MQ=255) ggcgtgcgCACTTCAGCCACAACCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 8:272633/134‑1 (MQ=255) gcgcACTTCCGCCACACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 4:278152/135‑1 (MQ=255) ttCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGGTCTCCAGCCTg > 1:196058/1‑135 (MQ=255) cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCa > 6:288282/1‑135 (MQ=255) cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCGTGGGCCTGCCGGTAACCGCCGCTGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCa > 4:252854/1‑135 (MQ=255) | AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCA > KT2440/5689692‑5689946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |