Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,036,532 C→A 8.9% Q183K (CAG→AAG)  CJ019_05653 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,036,5320CA 8.9% 145.1 / 5.4 54Q183K (CAG→AAG) CJ019_05653hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/26);  new base A (2/3);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

TGGCCGAACTCAAGGCCGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACG  >  KT2440/6036398‑6036658
                                                                                                                                      |                                                                                                                              
tGGCCGAACTCAAGGCCGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGAGCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGc                                                                                                                                <  1:20851/135‑1 (MQ=255)
gggCCGAACTCAAGGCTGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGc                                                                                                                                <  6:205567/134‑1 (MQ=255)
ggcccGAACTCACGGCCGGGCGCCAGCGCGAAGGCCCGGACCGCCCCCGGCGGAGCAACCACCGCCTGCACCACATGGCCCGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCGGGCGGCGCAGCGGc                                                                                                                                <  5:247570/132‑1 (MQ=255)
          cAAGGCCGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATtct                                                                                                                      >  7:31065/1‑135 (MQ=255)
           aaGGCCGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATtctc                                                                                                                     <  7:98159/135‑1 (MQ=255)
           aaGGCCGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATtctc                                                                                                                     <  8:38888/135‑1 (MQ=255)
            aGGCCGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATtctc                                                                                                                     <  2:96193/134‑1 (MQ=255)
            aGGCCGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATtctc                                                                                                                     <  8:59166/134‑1 (MQ=255)
               ccGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGAcc                                                                                                                 >  1:114742/1‑135 (MQ=255)
                      ccAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCgg                                                                                                          >  8:16514/1‑135 (MQ=255)
                              aaGGCCAGGACCTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGc                                                                                                  <  6:250005/135‑1 (MQ=255)
                                             cccGGCTGATCAACGAACGCCGGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGAcc                                                                                   <  5:35363/135‑1 (MQ=255)
                                              ccGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGAccc                                                                                  >  8:197050/1‑135 (MQ=255)
                                               ccgCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCCACCCCTTCGGCGACATGCAAGCCGAGCAGGACCca                                                                                 >  1:174698/3‑135 (MQ=255)
                                                   tGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAgc                                                                             <  7:8123/135‑1 (MQ=255)
                                                   tGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAgc                                                                             <  5:124281/135‑1 (MQ=255)
                                                    gATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAgcg                                                                            >  3:286136/1‑135 (MQ=255)
                                                        aacgaacgCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCt                                                                         >  4:132835/1‑134 (MQ=255)
                                                         acgaacgCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGGGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTg                                                                        <  6:118049/134‑1 (MQ=255)
                                                          cgaacgCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGa                                                                      <  1:28918/135‑1 (MQ=255)
                                                           gaacgCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAg                                                                     <  1:277765/135‑1 (MQ=255)
                                                             ccgCCTGGACAACATGGCCAGCTTAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCTACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCa                                                                   <  8:158358/134‑1 (MQ=255)
                                                               gCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAgg                                                                 <  4:270347/135‑1 (MQ=255)
                                                               gCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAgg                                                                 <  1:32257/135‑1 (MQ=255)
                                                                  tGGACAACATGGCCAGCGAAGTCACTCCCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCATAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGgaga                                                              <  1:11846/135‑1 (MQ=255)
                                                                  tGGACAACATGGCCAGCGAAGTCACTACACTGCGCGCCCTGGTGCCGCAGATGCTGTCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGgaga                                                              >  7:158358/1‑135 (MQ=255)
                                                                            ggCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGCAGCAGGAGATGGTActgct                                                    >  1:186619/1‑135 (MQ=255)
                                                                            ggCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGAAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCa                                                                   >  2:46022/1‑120 (MQ=255)
                                                                            ggCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGAAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCa                                                                   >  2:41248/1‑120 (MQ=255)
                                                                            ggCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGAAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCa                                                                   <  1:46022/120‑1 (MQ=255)
                                                                            ggCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGAAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCa                                                                   <  1:41248/120‑1 (MQ=255)
                                                                                    aaGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAg                                             >  1:151909/1‑134 (MQ=255)
                                                                                    aaGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGATGGCGGAGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAg                                             >  5:129875/1‑134 (MQ=255)
                                                                                        cACTCCCCTGCGCGCCCTGGTGCCGCAGATGCGGGCGGCGCTGCGGAATAAGATTCTCGACCGCTGCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAg                                        <  8:147971/135‑1 (MQ=255)
                                                                                        cACTACCCTGCGCGCCCTGTTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGATTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAg                                        <  4:51395/135‑1 (MQ=255)
                                                                                        cACTACCCTGCGCGCCCTGGGGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAg                                        <  6:247744/135‑1 (MQ=255)
                                                                                                tGCGCGCCGTGGTGCGGCAGATGCGGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTgg                                <  1:301712/135‑1 (MQ=255)
                                                                                                 gcgcgcCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTg                                 >  5:31874/1‑133 (MQ=255)
                                                                                                   gcgcCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGCCCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGCCGTGGc                               >  8:90348/1‑133 (MQ=255)
                                                                                                   gcgcCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGc                               >  4:226054/1‑133 (MQ=255)
                                                                                                    cgcCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCaga                            >  3:220835/1‑135 (MQ=255)
                                                                                                     gcCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCACGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCagag                           >  6:253150/1‑135 (MQ=255)
                                                                                                     gcCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCCCAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCaga                            >  8:154718/1‑134 (MQ=255)
                                                                                                     gcCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACg                                   >  7:65665/1‑127 (MQ=255)
                                                                                                     gcCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACg                                   <  8:65665/127‑1 (MQ=255)
                                                                                                     gcCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCaga                            >  4:226793/1‑134 (MQ=255)
                                                                                                          ggTGCCGCAGATGCTGGAGGCGCAGCGGCAGAAGATTCTCGAACGCTTCCGCGACATGCAACCCGAGCTGGCCCCACAGAGCCTGCAGCTCGAGATGGGACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACt                      >  3:104621/1‑135 (MQ=255)
                                                                                                           gggCCGCAGTGGCGGGGGGCGCAGCGGCTGACGTTTCTCGACCGCTGCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTc                     <  8:123753/133‑1 (MQ=255)
                                                                                                           gggCCGCAGATGCGGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTc                     <  8:258730/133‑1 (MQ=255)
                                                                                                              ccGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGCCCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGACCTCGAc                  >  4:43622/1‑135 (MQ=255)
                                                                                                                  tgttGCGGGCGGGGCTGCGGCAGATTATTCTCGAGCGCTTCGGGGTCTTGCAAGCCGAGCTGGCCCCTCAGCGCGGGGCGCAGGCCAGGGAACTGTTGGCCCATATAAGCTAGGTGGGAGAGGAACTCGACCGcc              <  4:104621/132‑1 (MQ=255)
                                                                                                                    aTGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGcc              >  7:123753/1‑133 (MQ=255)
                                                                                                                    aTGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGcc              >  6:298769/1‑133 (MQ=255)
                                                                                                                         ggcggcGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCAc       <  1:191682/135‑1 (MQ=255)
                                                                                                                           cggcgCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCAc       <  4:220835/133‑1 (MQ=255)
                                                                                                                              cgcAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACg  <  7:197050/135‑1 (MQ=255)
                                                                                                                              cgcAGCGGCAGAAGAGTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACg  <  8:209153/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                              
TGGCCGAACTCAAGGCCGGGCGCCAGCGCGAAGGCCAGGAACTGGCCCGGCTGATCAACGAACGCCTGGACAACATGGCCAGCGAAGTCACTACCCTGCGCGCCCTGGTGCCGCAGATGCTGGCGGCGCAGCGGCAGAAGATTCTCGACCGCTTCGGCGACATGCAAGCCGAGCTGGACCCACAGCGCCTGGAGCAGGAGATGGTACTGCTGGCCCAGAAAAGCGACGTGGCAGAGGAACTCGACCGCCTCAGCACCCACG  >  KT2440/6036398‑6036658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑