Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,097,340 C→A 7.6% A113D (GCC→GAC)  CJ019_05710 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,097,3400CA 7.6% 145.8 / 3.6 53A113D (GCC→GAC) CJ019_05710hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/29);  new base A (2/2);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

CCCACTGGCCGGCCTCGCCGCCGGTGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACCTGCTGCCACAGCCGCAC  >  KT2440/6097212‑6097471
                                                                                                                                |                                                                                                                                   
ccccCTGGCCGGCCTCGCCGCCGGTGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCcgcgcg                                                                                                                               <  5:235326/135‑1 (MQ=255)
  cACTGGCCGGCCTCGCCGCCGGTGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGcc                                                                                                                             >  5:17853/1‑135 (MQ=255)
   aCTGGCCGGCCTCGCCGCCGGTGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCg                                                                                                                            <  3:279171/135‑1 (MQ=255)
       gccggccTCGCCGCCGGTGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGc                                                                                                                        >  5:270656/1‑135 (MQ=255)
       gccggccTCGCCGCCGGTGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGc                                                                                                                        >  2:225299/1‑135 (MQ=255)
       gccggccTCGCCGCCGGTGGCCTGCTGGCTTCCATGTTCAGGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCGTGATCGTGGCGGTCCTCGCCGTGCGCGTGTTCGGCGTCATCGCCGCGCGCCGgcgc                                                                                                                        >  4:188191/1‑135 (MQ=255)
                  cgccgGTGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCGTGGACTTCCTGGTCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGACGCGAGCAGCAGcc                                                                                                             >  7:101123/1‑135 (MQ=255)
                       gTGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGCGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCaaa                                                                                                        <  2:170521/135‑1 (MQ=255)
                        tGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAAt                                                                                                       <  5:216820/135‑1 (MQ=255)
                            ctgctgGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGcc                                                                                                   <  7:25834/135‑1 (MQ=255)
                                tgGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCAt                                                                                                 <  7:40254/133‑1 (MQ=255)
                                tgGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCAt                                                                                                 <  1:54309/133‑1 (MQ=255)
                                    ttCCCTGTTCCTGGGCGACGGGTTCGAAGGCTGCCAGATCCTGGACTTCCTGATCGTGGCGCGCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAgg                                                                                           <  1:185247/135‑1 (MQ=255)
                                    ttCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAgg                                                                                           <  4:81117/135‑1 (MQ=255)
                                        aTGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCAt                                                                                       <  3:279632/135‑1 (MQ=255)
                                        aTGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCAt                                                                                       <  4:210057/135‑1 (MQ=255)
                                               tGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCg                                                                                 <  4:276038/134‑1 (MQ=255)
                                                   cGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTACCTCCTGTTCCGCTTCCTCGCCGCGCGCCGTCGCCAGCAGCCGCCGCAAGTGGCCATGCCCGGCCATGCGCCGATGCa                                                                            >  7:59656/1‑135 (MQ=255)
                                                     aCGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGc                                                                          <  1:77889/135‑1 (MQ=255)
                                                      cGGTTTCGAAGGCTTCCAGATCATTGACTTCCTGAGCGTGGCGCTCATCGCCTTCCTCGGGTTCCGCTTCATCGCCGCGCGCCGTCGACAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCg                                                                         <  8:227679/135‑1 (MQ=255)
                                                      cGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCg                                                                         >  8:33531/1‑135 (MQ=255)
                                                      cGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCg                                                                         <  3:113624/135‑1 (MQ=255)
                                                      cGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGGCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCg                                                                         <  5:277644/135‑1 (MQ=255)
                                                        ggtgcGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTg                                                                       <  3:158390/131‑1 (MQ=255)
                                                          ttCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAg                                                                     <  5:153959/135‑1 (MQ=255)
                                                          ttCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAg                                                                     <  3:149976/135‑1 (MQ=255)
                                                               aGGCATCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCa                                                                <  8:283236/135‑1 (MQ=255)
                                                                ggCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCAc                                                               <  3:280110/135‑1 (MQ=255)
                                                                 gCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCAGCGCCTGCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCAcg                                                              <  5:46364/135‑1 (MQ=255)
                                                                      cAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAg                                                         >  4:132455/1‑135 (MQ=255)
                                                                         aTCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCt                                                      <  7:82053/135‑1 (MQ=255)
                                                                               gACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCCGCCTGCGCAg                                                >  7:142768/1‑135 (MQ=255)
                                                                               gACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAg                                                >  5:95064/1‑135 (MQ=255)
                                                                                aCTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGc                                               <  8:101123/135‑1 (MQ=255)
                                                                                aCTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGACGCGCGCTGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGc                                               >  8:111883/1‑135 (MQ=255)
                                                                                aCTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGACGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGc                                               >  6:49594/1‑135 (MQ=255)
                                                                                       gATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGAt                                        >  2:284442/1‑135 (MQ=255)
                                                                                       gATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGAt                                        >  1:66670/1‑135 (MQ=255)
                                                                                               cgcTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCggtg                                >  8:237191/1‑135 (MQ=255)
                                                                                                 cTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTAggttgt                              >  3:184792/1‑135 (MQ=255)
                                                                                                     tCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCgg                          >  4:226262/1‑135 (MQ=255)
                                                                                                             cTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCAc                    <  8:81277/133‑1 (MQ=255)
                                                                                                              tCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCCCctg                 >  3:46398/1‑135 (MQ=255)
                                                                                                               cGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCAAGGCCATGAGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACct                  >  8:202621/1‑133 (MQ=255)
                                                                                                                 tgtTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGTGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACctgctg              <  1:149008/135‑1 (MQ=255)
                                                                                                                 tgtTCCGCTTCATCGACGCGCGCTGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACctgc                <  7:111883/133‑1 (MQ=255)
                                                                                                                 tgtTCCGCTTCATCGACGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACctgc                <  5:49594/133‑1 (MQ=255)
                                                                                                                  gtTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACCtgctgc             <  1:190990/135‑1 (MQ=255)
                                                                                                                       gCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACCTGCTGCCACAg        <  6:17853/135‑1 (MQ=255)
                                                                                                                         ttCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACCTGCTGCCACAGcc      <  5:282446/135‑1 (MQ=255)
                                                                                                                           cATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTGGATTTTGGGTGGTTAGGCCGCGCCAGCTGCCCCcgccgc    >  1:51323/1‑135 (MQ=255)
                                                                                                                           cATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGGTCGGCCGCCCCGGCTGCCACAGCCGc    >  8:274938/1‑135 (MQ=255)
                                                                                                                             tCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACCTGCTGCCACAGCCCCAc  >  7:39541/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                   
CCCACTGGCCGGCCTCGCCGCCGGTGGCCTGCTGGCTTCCATGTTCATGGGCGACGGTTTCGAAGGCTTCCAGATCATGGACTTCCTGATCGTGGCGCTCATCGCCTTCCTCGTGTTCCGCTTCATCGCCGCGCGCCGTCGCCAGCAGCAGCCGCAAATGGCCATGCCAGGCCATGCGCCGATGCAGCGTGAGGCTCACGCGCAGCCTGCGCAGCCGTCGATTTTCGGTGGTTCGGCCGCACCTGCTGCCACAGCCGCAC  >  KT2440/6097212‑6097471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑