Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,125,178 T→C 5.5% Q60R (CAG→CGG)  copB_2 ← Copper resistance protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,125,1780TC 5.5% 241.1 / 3.2 73Q60R (CAG→CGG) copB_2Copper resistance protein B
Reads supporting (aligned to +/- strand):  ref base T (41/28);  new base C (2/2);  total (43/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GGTCCATCCCCTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTGGGCGCAGCAGCACCCAGCATGCTGAA  >  KT2440/6125044‑6125308
                                                                                                                                      |                                                                                                                                  
ggTCCATCCCCTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCt                                                                                                                                    >  3:197482/1‑135 (MQ=255)
ggTCCATCCCCTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCt                                                                                                                                    >  1:218920/1‑135 (MQ=255)
  tCCATCCCCTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAAGAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGa                                                                                                                                  <  1:153835/135‑1 (MQ=255)
  tCCATCCCCTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTg                                                                                                                                   <  2:279934/134‑1 (MQ=255)
  tCCATCCCCTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGa                                                                                                                                  <  2:171622/135‑1 (MQ=255)
     aTCCCCTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGa                                                                                                                                  >  3:77875/1‑132 (MQ=255)
     aTCCCCTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGa                                                                                                                                  <  4:77875/132‑1 (MQ=255)
       ccccTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTg                                                                                                                             >  5:211626/1‑135 (MQ=255)
       ccccTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTg                                                                                                                             >  4:143413/1‑135 (MQ=255)
       ccccTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTg                                                                                                                             <  7:9313/135‑1 (MQ=255)
       ccccTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGGTTGGCCACAGGTTGACTGTGTTTCAGTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTg                                                                                                                             >  7:95068/1‑135 (MQ=255)
         ccTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGt                                                                                                                           >  1:14232/1‑135 (MQ=255)
         ccTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGt                                                                                                                           <  6:104767/135‑1 (MQ=255)
         ccTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGt                                                                                                                           >  7:42274/1‑135 (MQ=255)
            gCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGAGCCCCTCTGAGCGTGGTCCa                                                                                                                        >  2:256886/1‑135 (MQ=255)
             cATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGAGCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATATGAGCGTGGTCCAt                                                                                                                       >  3:104619/1‑135 (MQ=255)
              aTGCTGGCATGATCCATCTTGCTGTGGTCCTTGGGCGCAGGTTTGGCCACAGGTTGTCTGTGTTTCATTTTGCTGTGTTCAACTGGCGCTGGTTTCTTCTTCTGGTTACTGGCGACCATCTGAGCGTGGTCCTTg                                                                                                                      <  4:192077/135‑1 (MQ=255)
              aTGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATg                                                                                                                      <  8:174286/135‑1 (MQ=255)
              aTGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATg                                                                                                                      >  3:55258/1‑135 (MQ=255)
              aTGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCCGa                                                                                                                                  >  4:181847/1‑123 (MQ=255)
              aTGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCCGa                                                                                                                                  >  4:185582/1‑123 (MQ=255)
              aTGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCCGa                                                                                                                                  <  3:185582/123‑1 (MQ=255)
              aTGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCCGa                                                                                                                                  <  3:181847/123‑1 (MQ=255)
              aTGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTGTCTTCTGCTGCTTACTCGCGCCCCTCTGCGCGTGGTCCATg                                                                                                                      >  1:217576/1‑135 (MQ=255)
               tgctgcCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGc                                                                                                                     >  3:44589/1‑135 (MQ=255)
                   gcCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTg                                                                                                                  >  2:166876/1‑134 (MQ=255)
                   gcCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTg                                                                                                                  >  2:120297/1‑134 (MQ=255)
                    ccATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGt                                                                                                                           >  1:216471/1‑124 (MQ=255)
                    ccATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGt                                                                                                                           <  2:216471/124‑1 (MQ=255)
                    ccATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGc                                                                                                                >  2:43098/1‑135 (MQ=255)
                     cATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCa                                                                                                               >  6:169294/1‑135 (MQ=255)
                        gATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCt                                                                                                            >  4:277109/1‑135 (MQ=255)
                          tCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGATTCTTCTGCTGCTTACTCGCGCCCATCTGCGCGTGGTCCATGCCTGGCATcag                                                                                                           >  5:108088/1‑132 (MQ=255)
                                 tGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCg                                                                                                   >  3:158295/1‑135 (MQ=255)
                                 tGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCg                                                                                                   >  7:147665/1‑135 (MQ=255)
                                   cTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGc                                                                                                     <  5:169294/131‑1 (MQ=255)
                                      tgGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGa                                                                                              >  4:126724/1‑135 (MQ=255)
                                         tCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGc                                                                                            >  8:199565/1‑134 (MQ=255)
                                           cATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTTCTGCTTACTCGCGCCCATCTGAGCGTGGGCCATGCCTGGCATCTGGTCTGCCGCCCGAGCGGa                                                                                         >  6:1702/1‑135 (MQ=255)
                                           cATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGa                                                                                                                                  <  8:99717/94‑1 (MQ=255)
                                           cATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGa                                                                                                                                  >  7:99717/1‑94 (MQ=255)
                                           cATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGa                                                                                         >  4:260899/1‑135 (MQ=255)
                                           cATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATATGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGCGCGGa                                                                                         >  2:288384/1‑135 (MQ=255)
                                              gtgCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATc                                                                                      <  1:219857/135‑1 (MQ=255)
                                              gtgCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATc                                                                                      <  2:27982/135‑1 (MQ=255)
                                                gcgcAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCa                                                                                    >  3:105053/1‑135 (MQ=255)
                                                gcgcAGGGTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATc                                                                                      <  1:281042/133‑1 (MQ=255)
                                                   cAGGTTTGGCCACAGGTTGAGTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCCTCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGCATCCAtt                                                                                  >  1:83421/1‑134 (MQ=255)
                                                      gTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCa                                                                              <  3:143413/135‑1 (MQ=255)
                                                      gTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCa                                                                              >  4:58049/1‑135 (MQ=255)
                                                        ttGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATg                                                                            <  3:214468/135‑1 (MQ=255)
                                                            ccACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCt                                                                         <  4:136978/134‑1 (MQ=255)
                                                            ccACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCt                                                                         <  1:43098/134‑1 (MQ=255)
                                                                   ttGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGcc                                                                  >  6:277814/1‑134 (MQ=255)
                                                                   ttGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTAGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGccc                                                                 >  3:76909/1‑135 (MQ=255)
                                                                         gtgtTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGc                                                           >  1:32453/1‑135 (MQ=255)
                                                                           gtTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGc                                                           <  6:206514/133‑1 (MQ=255)
                                                                           gtTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGc                                                           <  3:126724/133‑1 (MQ=255)
                                                                             ttCCTTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGGGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGaa                                                       <  2:218920/135‑1 (MQ=255)
                                                                             ttCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGaa                                                       >  8:27344/1‑135 (MQ=255)
                                                                                 ttttGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATgg                                                    <  8:42274/134‑1 (MQ=255)
                                                                                 ttttGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATgg                                                    <  7:12319/134‑1 (MQ=255)
                                                                                          gATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCAtctc                                            <  7:27344/133‑1 (MQ=255)
                                                                                                    cgcgGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCaaa                                <  6:32706/135‑1 (MQ=255)
                                                                                                      cgGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGc                              >  7:284631/1‑135 (MQ=255)
                                                                                                        ggTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTg                            >  8:116437/1‑135 (MQ=255)
                                                                                                                ctgctgCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTGGGCgca                      >  8:23014/1‑133 (MQ=255)
                                                                                                                      ctTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATc                                                                                      >  1:118395/1‑63 (MQ=255)
                                                                                                                      ctTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATc                                                                                      <  2:118395/63‑1 (MQ=255)
                                                                                                                      ctTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTGGGCGCAGCAGCAcc              >  3:225221/1‑135 (MQ=255)
                                                                                                                       ttACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTGGGCGCAGCAGCAc               <  5:1702/133‑1 (MQ=255)
                                                                                                                                cccATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTGGGCgcagcag                  >  3:170377/1‑121 (MQ=255)
                                                                                                                                cccATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTGGGCgcagcag                  <  4:170377/121‑1 (MQ=255)
                                                                                                                                   aTCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTGGGCGCAGCAGCACCCAGCATGCtgaa  >  6:153086/1‑134 (MQ=255)
                                                                                                                                   aTCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGCAGCGGAATCCCTTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTGGGCGCCGCCGCACCCCGCATGCtgaa  >  6:169403/1‑134 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GGTCCATCCCCTGCATGCTGCCATGATCCATCTTGCTGTGGTCCATGTGCGCAGGTTTGGCCACAGGTTGACTGTGTTTCATTTTGCTGTGATCAACAGGCGCGGGTTTCTTCTGCTGCTTACTCGCGCCCATCTGAGCGTGGTCCATGCCTGGCATCTGGTCTGCCGACGGAGCGGAATCCATTTCCATGGCTCCGTGCCCCATTGCCGAATGGTCCATCTCCTCAGCAGCAAAGCTGGGCGCAGCAGCACCCAGCATGCTGAA  >  KT2440/6125044‑6125308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: