Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,066,751 T→A 5.6% K14* (AAA→TAA)  CJ019_04758 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,066,7510TA 5.6% 261.9 / 3.8 88K14* (AAA→TAA) CJ019_04758hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/56);  new base A (2/3);  total (29/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

AAAGGCTGAGGCTGCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCTTTTACACACTCTAAGATAATCCGTATCGTTCGA  >  KT2440/5066620‑5066884
                                                                                                                                   |                                                                                                                                     
aaaGGCTGAGGCTGCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAAtttttt                                                                                                                                     >  8:305231/1‑134 (MQ=255)
aaaGGCTGAGGCTGCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAAtttttt                                                                                                                                     >  6:305148/1‑134 (MQ=255)
  aggctgaggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGa                                                                                                                                  <  5:170107/135‑1 (MQ=255)
  aggctgaggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGa                                                                                                                                  <  8:144187/135‑1 (MQ=255)
  aggctgaggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGa                                                                                                                                  <  3:50931/135‑1 (MQ=255)
  aggctgaggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGa                                                                                                                                  <  3:226333/135‑1 (MQ=255)
  aggctgaggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGa                                                                                                                                  <  1:318164/135‑1 (MQ=255)
     ctgaggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCa                                                                                                                               <  2:185585/135‑1 (MQ=255)
         ggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTATAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAg                                                                                                                             <  1:58412/133‑1 (MQ=255)
         ggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAg                                                                                                                             <  6:177010/133‑1 (MQ=255)
         ggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAg                                                                                                                             <  6:273926/133‑1 (MQ=255)
         ggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAg                                                                                                                             <  3:236158/133‑1 (MQ=255)
         ggctgCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAg                                                                                                                             <  2:370514/133‑1 (MQ=255)
            ttctTTGAATTTAACCCTGGGCCTGCAATTCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGAtt                                                                                                                        <  6:7068/133‑1 (MQ=255)
            tgctTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAATGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGAtt                                                                                                                        <  5:193437/135‑1 (MQ=255)
                ttGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATtata                                                                                                                     >  3:384624/1‑134 (MQ=255)
                 tGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATAc                                                                                                                    >  3:84225/1‑134 (MQ=255)
                 tGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATAc                                                                                                                    >  8:284678/1‑134 (MQ=255)
                    aTTTACCCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACaaa                                                                                                                 <  6:71700/134‑1 (MQ=255)
                    aTTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACaaa                                                                                                                 <  7:287598/134‑1 (MQ=255)
                    aTTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACaaa                                                                                                                 <  6:77334/134‑1 (MQ=255)
                          cccTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGc                                                                                                          <  5:321751/135‑1 (MQ=255)
                           ccTGGGCCTGCAATGCTTGCTAGAAGTCATACCGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTAGACAAATgaga                                                                                                            >  6:144357/1‑132 (MQ=255)
                           ccTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGAtt                                                                                                                        >  5:245099/1‑120 (MQ=255)
                           ccTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGAtt                                                                                                                        <  6:245099/120‑1 (MQ=255)
                           ccTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATgaga                                                                                                            >  6:264865/1‑132 (MQ=255)
                                gcatGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGTTAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAATGGATTATACAAATGAGAGCagag                                                                                                      <  6:21723/130‑1 (MQ=255)
                                gCCTTCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGaggg                                                                                                    <  2:216801/135‑1 (MQ=255)
                                gCCTGCAATGCTTGCTAGAAGTCATAACGCTCTAAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGaggg                                                                                                    <  7:359783/135‑1 (MQ=255)
                                  cTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGagggg                                                                                                   >  7:222018/1‑134 (MQ=255)
                                  cTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGaggg                                                                                                    >  1:378033/1‑133 (MQ=255)
                                  cTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGaggg                                                                                                    <  2:378033/133‑1 (MQ=255)
                                   ttcAATGCTTGCTAGAAGTCATAACTCTCTCAATAATTTGCTCTTTCTGATAGTTACGGTTCGTTTAGTCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGTATTATACATATGAGAGCAGagtggct                                                                                                 <  8:175082/133‑2 (MQ=255)
                                        tgcttgctAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGaggggcaagggg                                                                                            <  2:305374/135‑1 (MQ=255)
                                        tgcttgctAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATATTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGaggggcaaggg                                                                                             >  5:248441/1‑134 (MQ=255)
                                             gctAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACAAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGa                                                                                       >  8:157263/1‑135 (MQ=255)
                                                aGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGTGCAAGGGGCAAGAATc                                                                                    >  2:153653/1‑135 (MQ=255)
                                                  aaGTCATAACGCTCTCAAAAATTTGCTCTTGCTTATAGAAACGTTTCGTTTCTGCAAACCTTGAGCGATTTTCTTAAATTATTTTGACCAAGGGATTATACAAATGAGATCAGAGGGGCAAGGTGCAAGAATCCt                                                                                  >  2:210458/1‑135 (MQ=255)
                                                       aTAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATgaga                                                                                                            >  3:377388/1‑104 (MQ=255)
                                                       aTAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATgaga                                                                                                            <  4:377388/104‑1 (MQ=255)
                                                          aCGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTcgcc                                                                           >  6:266642/1‑134 (MQ=255)
                                                          aCGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTcgcc                                                                           >  4:318030/1‑134 (MQ=255)
                                                          aCGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTAGAGCGAATTTCTGAAATTTTGTTGACCAAGCGATTATACAAATGAGAGCAGAGCGGCAACGGGAAAGAATCCTGGTcgcc                                                                           >  4:61855/1‑134 (MQ=255)
                                                                tcAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGTGATTATAAAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCaaaaa                                                                    >  8:244887/1‑135 (MQ=255)
                                                                  aaaaaTTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCaaaaaa                                                                   >  8:157387/1‑134 (MQ=255)
                                                                  aaaaaTTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCaaaaaa                                                                   >  2:221125/1‑134 (MQ=255)
                                                                  aaaaaTTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAAt                                                                  >  8:265485/1‑135 (MQ=255)
                                                                   aaaaTTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAAt                                                                  >  2:42184/1‑134 (MQ=255)
                                                                    aaaTTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTc                                                                <  4:84225/135‑1 (MQ=255)
                                                                             ctTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGATGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTaa                                                        >  7:56521/1‑134 (MQ=255)
                                                                             ctTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTaa                                                        >  1:353597/1‑134 (MQ=255)
                                                                                gCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTaaaaa                                                     <  1:42184/134‑1 (MQ=255)
                                                                                 cTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTaaaaaa                                                    <  5:294131/134‑1 (MQ=255)
                                                                                  tGATATAAACGCTTCGTTGAGGCAAACATTGTGCTATTTTCTGAAATTTTTTTGACCAATTGATTATACAAATGAGAGCAGAGGGGCATGGGGCAAGATTCCTGGTCGGCGCAAAAAATTCAGACGTAAAAAAg                                                   <  6:249995/134‑1 (MQ=255)
                                                                                  tGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAg                                                   <  5:264865/134‑1 (MQ=255)
                                                                                  tGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAg                                                   <  1:11379/134‑1 (MQ=255)
                                                                                  tGATAGAAACGGGTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGc                                                  <  4:84827/135‑1 (MQ=255)
                                                                                          ccggggcGTTTCGGCACACCTTTAGAGATTTTGTGAAATTTTTTTGACCAAGGGATTATACAAAGGAGACCCGCGGGCCAAGGGGCAACAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTa                                          <  6:248441/129‑1 (MQ=255)
                                                                                             gTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTATTTGACCAAGGGATTATACAAATGAGAGCAGaggg                                                                                                    >  4:268782/1‑74 (MQ=255)
                                                                                             gTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTATTTGACCAAGGGATTATACAAATGAGAGCAGaggg                                                                                                    >  4:263797/1‑74 (MQ=255)
                                                                                             gTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTATTTGACCAAGGGATTATACAAATGAGAGCAGaggg                                                                                                    <  3:268782/74‑1 (MQ=255)
                                                                                             gTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTATTTGACCAAGGGATTATACAAATGAGAGCAGaggg                                                                                                    <  3:263797/74‑1 (MQ=255)
                                                                                                 gTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCt                                   >  4:184136/1‑135 (MQ=255)
                                                                                                   ttCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtt                                  >  5:241717/1‑134 (MQ=255)
                                                                                                    tctgCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  6:101570/132‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  3:116591/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  8:222018/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  3:158316/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  3:285639/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  8:146776/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  7:70680/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  3:307076/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  3:47901/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  3:78172/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  7:213456/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  5:266642/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  6:37535/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  1:315000/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  2:57168/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  2:372051/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGACAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  5:215525/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGCGATTATACAACTGAGAGCCGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  5:144357/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTTTCTGAAATTTATTTGACCAAGCGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  1:231152/134‑1 (MQ=255)
                                                                                                     cGGCAAACCTTGAGCGATTATCTGAAATTTTGTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCtttt                                <  4:249656/134‑1 (MQ=255)
                                                                                                       gCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCTTTTac                              <  7:30325/134‑1 (MQ=255)
                                                                                                        cAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCTTTTacac                            <  3:285170/135‑1 (MQ=255)
                                                                                                               tGATCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATACTGGGCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCTTTTACACACTCTaa                     <  6:216243/135‑1 (MQ=255)
                                                                                                                   cGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCTTTTACACACTCTAAGATa                 >  6:18334/1‑135 (MQ=255)
                                                                                                                       tttCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCTTTTACACACTCTAAGATAATcc             <  7:284678/135‑1 (MQ=255)
                                                                                                                         tCTGAAATATTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCTTTTACACACTCTAAGATAATCCGt           >  6:217845/1‑135 (MQ=255)
                                                                                                                                   ttttGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCTTTTACACACTCTAAGATAATCCGTATCGTTCGa  <  1:153653/134‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
AAAGGCTGAGGCTGCTTTGAATTTAACCCTGGGCCTGCAATGCTTGCTAGAAGTCATAACGCTCTCAAAAATTTGCTCTTGCTGATAGAAACGGTTCGTTTCGGCAAACCTTGAGCGATTTTCTGAAATTTTTTTGACCAAGGGATTATACAAATGAGAGCAGAGGGGCAAGGGGCAAGAATCCTGGTCGCCGCAAAAAATTCAGACGTAAAAAAGCCCCAGCTAAGGGGCTTTTACACACTCTAAGATAATCCGTATCGTTCGA  >  KT2440/5066620‑5066884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑