Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,149,443 G→A 5.8% A26T (GCC→ACC)  yhaJ_3 → HTH‑type transcriptional regulator YhaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,149,4430GA 5.8% 226.6 / 2.2 69A26T (GCC→ACC) yhaJ_3HTH‑type transcriptional regulator YhaJ
Reads supporting (aligned to +/- strand):  ref base G (27/38);  new base A (2/2);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTTCTTCATGGGCCGCTCTCCTTGAAAGAGCGCAGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCGGCAATGTGCTGC  >  KT2440/5149309‑5149575
                                                                                                                                      |                                                                                                                                    
caTTTCTTCATGGGCCGCTCTCCTTGAAAGAGCGCAGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAgg                                                                                                                                      >  5:378056/1‑135 (MQ=255)
   ttcttcATGGGCCGCTCTCCTGGAAAGAGCGCACGTTAGGACACAAGGAGCGAACGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGgccg                                                                                                                                   <  8:136015/135‑1 (MQ=255)
   ttcttcATGGGCCGCGCTCCTTCAAAGAGCGCAGCTTAGGACAACCGGAGCGAAACATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGgccg                                                                                                                                   <  8:257362/135‑1 (MQ=255)
      ttcATGGGCCGCTCTCCTTGAAAGAGCGCAGCTTAGGACACCAGGAGCGAAAGATCAAAGCGCCGCGCGTAACCCTTGACCAGGGGCGAACCCTGCAAGCAGTGGGCGATCACGGCGGGTTTGCCCAGgccgcc                                                                                                                                 <  4:61417/134‑1 (MQ=255)
        cATGGGCCGCTCTCCTTGAAAGAGCGCAGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAg                                                                                                                              >  6:15322/1‑135 (MQ=255)
             gCCGCTCTCCTTGAAAGAGCGCAGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCtt                                                                                                                         >  4:242630/1‑135 (MQ=255)
             gCCGCTCTCCTTCAAACAGCGCAGCTTAGCACACCAGGACCCAAACATCAACGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGGCGAGCACGGCGGGTTTGCCCAGGCCGCCGAGGCCtt                                                                                                                         <  5:307242/135‑1 (MQ=255)
             gCCGCTCGCCTTGAACGCGCGCAGCTTCGCACACCCGGCGCGAAAGAGCAACGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGGCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCtt                                                                                                                         <  1:57980/135‑1 (MQ=255)
                             gcgggcAGCTTAGGACAACAGGAGCGAAATATAAATGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCa                                                                                                         <  8:169663/131‑1 (MQ=255)
                             gcgcgcATGTTAGGAAAACAGGAGCTAAAGATGAAAGCGCCGCGCGTAACCCTTGACCTGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCa                                                                                                         <  7:355038/133‑1 (MQ=255)
                             gcgcgcAGCTTAGCACACCCGGAGCCACAGTTCACCGCGCGGGGCGTAACCCTTGACCCGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCa                                                                                                         <  2:169891/133‑1 (MQ=255)
                             gagCGCAGCTTAGTAAAACAGGCGCGAAAGATGAACGCGCCGCGCGTAACCCTTGACCAGGGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCa                                                                                                         <  2:348444/135‑1 (MQ=255)
                             gagCGCAGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCa                                                                                                         <  7:381600/135‑1 (MQ=255)
                             gagCGCAGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCa                                                                                                         <  3:331107/135‑1 (MQ=255)
                             gagCGCAGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCa                                                                                                         <  7:386392/135‑1 (MQ=255)
                               gcgcAGCTTAGGACAACCGGAGCGAAAGATGACAGCGCCGCGCGTAACCCTGGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATc                                                                                                       <  4:117852/135‑1 (MQ=255)
                                 gcAGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCAGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCgg                                                                                                     >  7:169663/1‑135 (MQ=255)
                                   aGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCgg                                                                                                     >  5:167039/1‑133 (MQ=255)
                                   aGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCgg                                                                                                     >  1:101240/1‑133 (MQ=255)
                                   aGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCgg                                                                                                     >  5:326408/1‑133 (MQ=255)
                                   aGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCgg                                                                                                     >  6:175160/1‑133 (MQ=255)
                                   aGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCgg                                                                                                     >  2:331795/1‑133 (MQ=255)
                                   aGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCgg                                                                                                     >  1:348444/1‑133 (MQ=255)
                                   aGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTc                                                                                                   >  7:301859/1‑135 (MQ=255)
                                               caGGAGCGAAAGATGAATGCGCCTCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTg                                                                                       <  3:304634/135‑1 (MQ=255)
                                                 ggAGCTAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCGCGCAGTCATCGGTCAGCTACACCGTGGc                                                                                     <  8:83962/135‑1 (MQ=255)
                                                  gAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGcc                                                                                    >  7:39171/1‑135 (MQ=255)
                                                  gAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGAGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCATGCACCGCTCGCACTCATCGGTCAGCTACCCCGTGGcc                                                                                    >  2:175959/1‑135 (MQ=255)
                                                   aGCCAAAGATGAACGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGGCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGccc                                                                                   <  2:369629/135‑1 (MQ=255)
                                                     cGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGc                                                                                 <  8:39171/135‑1 (MQ=255)
                                                       aaaGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCAt                                                                               >  3:340666/1‑135 (MQ=255)
                                                                cgcgcCGCGAGTAACCGTTGACCAGTGGGGACCCCTGCAAGCAGTTGTCGATCACGGCGGGTTTGCCCAGGCCGACGAGGCCTTGCACCGCTCTCAGTCATAGGTCAGCTACACCGTGGCCCGAATGCAGGagca                                                                      <  2:223132/134‑1 (MQ=255)
                                                                        gcgTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCg                                                              <  6:326408/135‑1 (MQ=255)
                                                                        gcgTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGACCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTgg                                                                 >  3:112186/1‑132 (MQ=255)
                                                                        gcgTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGACCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTgg                                                                 >  3:117927/1‑132 (MQ=255)
                                                                        gcgTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGACCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTgg                                                                 <  4:117927/132‑1 (MQ=255)
                                                                        gcgTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGACCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTgg                                                                 <  4:112186/132‑1 (MQ=255)
                                                                          gTAACCCTTGCCCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTc                                                            <  3:64286/135‑1 (MQ=255)
                                                                                     ccAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATc                                                                                                       >  4:103999/1‑81 (MQ=255)
                                                                                     ccAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATc                                                                                                       <  3:103999/81‑1 (MQ=255)
                                                                                     ccAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTgc                                                  >  6:65286/1‑134 (MQ=255)
                                                                                        gTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCAt                                              <  8:383598/135‑1 (MQ=255)
                                                                                         tGGCGAACCCTGCAAGCAGTGGTCGTTCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATc                                             <  1:327672/135‑1 (MQ=255)
                                                                                           gCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGa                                           <  4:26941/135‑1 (MQ=255)
                                                                                            cGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGAt                                          >  8:335472/1‑135 (MQ=255)
                                                                                            cGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGAt                                          >  6:219245/1‑135 (MQ=255)
                                                                                            cGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGAt                                          >  3:170583/1‑135 (MQ=255)
                                                                                            cGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGAt                                          >  3:316080/1‑135 (MQ=255)
                                                                                            cGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATCCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGAt                                          >  8:232863/1‑135 (MQ=255)
                                                                                              aaCCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTgg                                                                 <  1:294013/110‑1 (MQ=255)
                                                                                              aaCCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTgg                                                                 >  2:294013/1‑110 (MQ=255)
                                                                                              aaCCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATgg                                        <  5:129267/135‑1 (MQ=255)
                                                                                                cccTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGGCCCGCTGTTGCGCATCGATGGcc                                      <  7:31332/135‑1 (MQ=255)
                                                                                                 ccTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGACGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCCTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGcc                                      >  5:98932/1‑134 (MQ=255)
                                                                                                   tGCAAGCAGGGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCa                                   <  1:91865/135‑1 (MQ=255)
                                                                                                      aaGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAgg                                >  4:282360/1‑135 (MQ=255)
                                                                                                        gCAGTGGTCGATCACGGCGGGTTCGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGGCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCg                              <  4:87672/135‑1 (MQ=255)
                                                                                                             ggTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGc                          >  4:227176/1‑134 (MQ=255)
                                                                                                              ggcGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTg                        <  4:261083/133‑1 (MQ=255)
                                                                                                              gTCGATCACGGCGGGTTTGCCCAGGCCGACGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTg                        <  6:98932/135‑1 (MQ=255)
                                                                                                                cGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGAc                      <  2:247302/135‑1 (MQ=255)
                                                                                                                   tCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGa                   <  3:18082/135‑1 (MQ=255)
                                                                                                                   gcACGGCGGGTTTGCCCAGGCCGGCTAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGa                   <  6:312963/134‑1 (MQ=255)
                                                                                                                      cggcggGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATCCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGc                <  7:232863/135‑1 (MQ=255)
                                                                                                                       ggcggGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCATGCCCCGCATGCAGGAGCAGCTGGGCGTCCCGCAGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGcc               >  7:172101/1‑135 (MQ=255)
                                                                                                                        gcggGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCg              <  3:180758/135‑1 (MQ=255)
                                                                                                                        gcggGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCg              <  8:279024/135‑1 (MQ=255)
                                                                                                                         cggGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCgg             >  5:228684/1‑135 (MQ=255)
                                                                                                                           ggTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACCCCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCGGCa           <  2:3568/135‑1 (MQ=255)
                                                                                                                           ggTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCGGCa           <  6:179193/135‑1 (MQ=255)
                                                                                                                           ggTGTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGGCATCGGGCAGCTACACCGTGGCCCGCATTCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCGGCa           <  6:238811/135‑1 (MQ=255)
                                                                                                                                 cccAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCGGCAAtgtg      <  5:65286/134‑1 (MQ=255)
                                                                                                                                    aGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCCGCTACACCGTGGCCCGCATGCAGGAGCAGCAGGGCGTCCCGCGGGTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCGGCAATGtgctgc  >  4:354061/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                    
CATTTCTTCATGGGCCGCTCTCCTTGAAAGAGCGCAGCTTAGGACAACAGGAGCGAAAGATGAAAGCGCCGCGCGTAACCCTTGACCAGTGGCGAACCCTGCAAGCAGTGGTCGATCACGGCGGGTTTGCCCAGGCCGCCGAGGCCTTGCACCGCTCGCAGTCATCGGTCAGCTACACCGTGGCCCGCATGCAGGAGCAGCTGGGCGTCCCGCTGTTGCGCATCGATGGCCGCAAGGCGGTGCTGACCGAGGCCGGCAATGTGCTGC  >  KT2440/5149309‑5149575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑