| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,689,794 | T→G | 48.2% | R228R (CGT→CGG) | pyrC' → | Dihydroorotase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,689,794 | 0 | T | G | 48.2% | 5.9 / 3.9 | 28 | R228R (CGT→CGG) | pyrC' | Dihydroorotase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (4/10); new base G (8/6); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTC > KT2440/5689663‑5689926 | gACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGCTGGGGGACCAGAGCggcgggcgggcg > 8:282549/1‑135 (MQ=255) aCCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGgcgc > 4:194278/1‑135 (MQ=255) ccTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACTGAGCGGCGtgggtgcggc > 1:231763/1‑133 (MQ=255) ccTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCa > 4:201176/1‑135 (MQ=255) cccAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTc < 1:56910/134‑1 (MQ=255) ggcCTGGCCCCCGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACAGCGCGGCGTGGGGGCGCCCGTCAGccccctc > 8:243351/1‑130 (MQ=255) tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCCAATCACCa > 1:281836/1‑135 (MQ=255) tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCGTCCGCCCGCTCAccc > 7:295123/1‑134 (MQ=255) cACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTAAGCCCCATCACCAGcccc > 7:386253/1‑135 (MQ=255) aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGCCCGTGCGCCCCATCACCACCCCCCCTgg > 6:318894/1‑135 (MQ=255) aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCACATCACCAGCGCCCGTgg > 4:254717/1‑135 (MQ=255) gggCCAGGCCCTGCTTCGGCGGCCTGCGGGGCATCCCGGAGAGCGCCGAAACCTTGGCCCGGGCGCGAAACCGGCTGCTGGGTGAACCGAGGGGCGTGCGTGCGCAATTCAGCCAGTTCACCTGCGCCGGGGgcg < 8:389258/133‑1 (MQ=255) tgaccAGCTGCCTCGGCCGGCGGGGAAGCCCGGAGAGCGCCTAAACCGTGGCCCGGGCGCGCAACCTGCTGCTGGTGCAACAGAGCGGCGTGCGGGCGCACTTCACCCAGATCACCAGCGCCCGGGGCGCGCGGc < 8:360956/132‑1 (MQ=255) ccAGCTTCCGCGGCCTGCCGGGCACCCCGGAGAGCGCCGAACCCGGGGCCCGGCCGCGCAACCTGCTGCTGGTGCAACAGAGCGGCGGGCGTGCGCACTTCCGCCAGAGCCCCCGCGCCCGGGGCGCGCGGCt < 1:66459/133‑1 (MQ=255) ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCCCTTCACCCACATCACCAGCCCCCCGGGCGCGCGGCGGCTCgcccc > 7:383690/1‑134 (MQ=255) ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGGGGGGGGCCGTCAGCCCGCTCACCCACGCCCGGGGGGCGCGGGGGGTCgcccc > 5:126251/1‑134 (MQ=255) gcggccggcCGGGCATCCCGGAGAGCGCCGAACCCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTGCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCgcccagg < 8:295123/134‑1 (MQ=255) ccggccggcCGGGCACCCCGGCGCCCCCCTAAACCGGGGCCGTGGCGCCCACCCGGCTGGGGGGGCACCAGCGCGGCGGGCGGGCGAACTCCAGCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCgcccagg < 4:279543/134‑1 (MQ=255) cTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGACCACCAGCGCCCGTGGCGCGCGGCTGATCgcccaggcccag < 3:316518/135‑1 (MQ=255) gCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGGGCCCTTAAGGCCCCACAACACCGCCCGGTGCGCCGGGGGGATCgcccacgcccccgc > 1:317526/1‑131 (MQ=255) cgcCGAAAGGGGGGCCGTGGCGGGCAACGGGCGGGTGGGGCAACTCCGCGGGGGGCTGGCGCAGTACCCCCTGAACCCCAGCGCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 5:254794/135‑1 (MQ=255) accccGTGGCCCGGCCGCGCACCCTGATCCGGGGGGAACAGAGGGGCGGGCGTGGGATATTCCGCCCGCGCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCGCAGGCCTTGGGCCTGCCGGTAAccgccg < 8:326820/132‑1 (MQ=255) aCCGTGGCCGTGGCGCGCAACGTGCTGGTGGTGCAACTGCGCGGCGGGCGGGGGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg < 1:36502/133‑1 (MQ=255) gTGGCCCTGGGGCGCAACCTGGGGCTGGTGTAACTGTGGGGCGGGCGTGGGCAGTCCTCCCTCAACACCCCGGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:128558/135‑1 (MQ=255) gTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 3:194278/135‑1 (MQ=255) gTGGCCCTGGCGCGCAACCTGCTGCGGGTGGACCAGAGCGGCGGGCGTGCGAACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 2:13697/135‑1 (MQ=255) cccTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGCCCATACCCCCGATCCCCCGCGCCCCGGGCGCGCGGCGGGTCGCCCCCGCCCCCGCCGTGGGCCGGGCCGGAACCCCCGCTGTAgc > 3:354595/1‑133 (MQ=255) ccTGGCGCGCAACCTGCTGCTGGTGCAACAGCGCGGCGTGCGTGCGCACTTCAGCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt < 3:254717/135‑1 (MQ=255) cAACCTGGTGCTGGTGCAACAGAGGGGCGTGCGTGGGAATTTACGCCATAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt < 8:310938/135‑1 (MQ=255) cggcggGCGTGCGCACTTCCGCCCTATCCCCAGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 8:269116/135‑1 (MQ=255) cgtgcgCACTGCAGCCCGAGCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc < 8:363758/135‑1 (MQ=255) | GACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTC > KT2440/5689663‑5689926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |