Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,116,475 T→A 6.6% intergenic (‑59/+31) CJ019_01056 ← / ← capB_1 hypothetical protein/Cold shock protein CapB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,116,4750TA 6.6% 189.1 / 3.9 61intergenic (‑59/+31)CJ019_01056/capB_1hypothetical protein/Cold shock protein CapB
Reads supporting (aligned to +/- strand):  ref base T (30/27);  new base A (2/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

AGAACATCGCTGCCAGGCGCCGGCCCAGGCCGACCGGTGGAAAATCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTTTCAGGCTCTTGAAGCCGTT  >  KT2440/1116341‑1116601
                                                                                                                                      |                                                                                                                              
aGAACATCGCTGCCAGGAGCCGGCCCAGTCCGACCGGTGGAAAATCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCAAAAAGACGAGCACACTATTCTACGGGCAAAAAAAAGCCCATGCACtt                                                                                                                                >  7:74604/1‑135 (MQ=255)
  aaCATCGCTGCCAGGCGCCGGCCCAGGCCGACCGGTGGAAAATCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTg                                                                                                                               >  8:237712/1‑134 (MQ=255)
  aaCATCGCTGCCAGGCGCCGGCCCAGGCCGACCGGTGGAAAATCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTg                                                                                                                               >  2:139124/1‑134 (MQ=255)
          tGCCAGGCGCCGGCCCAGGCCGACCGGTGGAAAATCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCt                                                                                                                      >  3:256925/1‑135 (MQ=255)
            ccAGGCGCGTGCGCAGGCCGACGGGTTTAAAATCCCCCTGGGTAGTGAGTAGAGGCTTGTACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCttt                                                                                                                    <  8:167327/135‑1 (MQ=255)
            ccAGGCGCCGGCCCAGGCCGACCGGGGGAAAATCCCCCTGGGTAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCttt                                                                                                                    <  8:248352/135‑1 (MQ=255)
                      gCCCAGGCCGACCGGTGGAAAATCCCCCGGGGGTGGTTGTCGCGGCTTGCACATGCAGGCCGCGCCAAACGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTg                                                                                                          <  4:214070/135‑1 (MQ=255)
                           ggCCGACCGGTGGAAAATCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCt                                                                                                     <  7:237712/135‑1 (MQ=255)
                            gCCGACCGGTGGAAAATCCCGAGTGGGTGTGATTAGAGTATTGCACATGCAGGACGCTCCAAAAGACGAGCCCACTATTATACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTg                                                                                                    <  8:74604/135‑1 (MQ=255)
                                aCCGGTGGAAAATCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATc                                                                                                <  6:173272/135‑1 (MQ=255)
                                         aactCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGtt                                                                                       <  6:186098/132‑1 (MQ=255)
                                         aaaTCCCCCTGGGGAGTTAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGtt                                                                                       <  2:304184/135‑1 (MQ=255)
                                         aaaTCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGtt                                                                                       <  7:201425/135‑1 (MQ=255)
                                          aaTCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTg                                                                                      <  8:197741/135‑1 (MQ=255)
                                                ccTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCtt                                                                                <  8:387143/135‑1 (MQ=255)
                                                ccTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCtt                                                                                <  6:353225/135‑1 (MQ=255)
                                                        gtgagtAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCt                                                                        <  2:295207/135‑1 (MQ=255)
                                                            gtAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCAt                                                                    >  7:231944/1‑135 (MQ=255)
                                                              agagGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGACCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGc                                                                  >  7:128257/1‑135 (MQ=255)
                                                                tggCTGGGACATGCCGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCt                                                                <  8:186938/134‑1 (MQ=255)
                                                                cggCTGGGACATGCAGGCCGCTCCAAACCACGAGCCCACTATTCTACGGCCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCt                                                                <  1:224517/134‑1 (MQ=255)
                                                                agGGTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCt                                                                <  6:77878/135‑1 (MQ=255)
                                                                agGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCt                                                                <  3:346857/135‑1 (MQ=255)
                                                                   cTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCCATTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGCACCTTGACCGCCTGCATGCCTTTc                                                             >  1:170628/1‑135 (MQ=255)
                                                                       gACATGCAGGACGCGCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTg                                                           <  5:71971/133‑1 (MQ=255)
                                                                        aCATGCAGGCCGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTg                                                                               <  8:36394/112‑1 (MQ=255)
                                                                        aCATGCAGGACGCTCCAAAAGACGAGCCCCCTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTg                                                                               >  7:36394/1‑112 (MQ=255)
                                                                        aCATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGcc                                                        >  8:10428/1‑135 (MQ=255)
                                                                        aCATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGcc                                                        >  7:192634/1‑135 (MQ=255)
                                                                        aCATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGcc                                                        >  7:235068/1‑135 (MQ=255)
                                                                        aCATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGcc                                                        >  6:201408/1‑135 (MQ=255)
                                                                          aTGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCtt                                                      >  3:6672/1‑135 (MQ=255)
                                                                          aTGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACACGTTGAACCTTGTCAGCCTGCATGCCTGTCTGGCAtt                                                      >  6:22641/1‑135 (MQ=255)
                                                                          aTGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTAGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCtt                                                      >  1:158730/1‑135 (MQ=255)
                                                                          aTGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGACACTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCtt                                                      >  7:69759/1‑135 (MQ=255)
                                                                             cAGGACGCTCAAAAAGACGAGCCCACTATTCTACGGGCAAAAACAAGCCCCTGCACTTGGCAGGGTCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGa                                                   <  8:295212/135‑1 (MQ=255)
                                                                               ggccGCTCCAAAACACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACt                                                 <  7:382776/132‑1 (MQ=255)
                                                                               ggACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACt                                                 >  6:326728/1‑135 (MQ=255)
                                                                               ggACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACt                                                 >  1:225437/1‑135 (MQ=255)
                                                                                   gCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCtt                                             >  6:103743/1‑135 (MQ=255)
                                                                                     tCCAAATGACGCGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCGTTTCAAAGTCTGGCGCTGGATCAGCCTGGTTGCAGCTTGTCCGCCTGCCTGCCGTTGGGGCCTTGGCCGGCTTc                                            >  6:61200/1‑134 (MQ=255)
                                                                                        aaaaGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAg                                        >  7:110687/1‑135 (MQ=255)
                                                                                             actaGCACAATATTCTTCGGGCAAAAAAAAGCCACTGCTCTTGGCAGGGGCTTTTCATAGTCTTGCGCTGGATCAGACAGTTTGAACCTTTTCAGACTGCATGCCTTTCTGGCCTTGGACTGCTTCGAATGTGAc                                   <  6:156397/132‑1 (MQ=255)
                                                                                               gAGCCCACTATTCTACGTGCAAAAAACAGCCCCTGCACTTGGCAGGGTCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACtt                                 <  4:90981/135‑1 (MQ=255)
                                                                                               gAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACtt                                 <  4:6672/135‑1 (MQ=255)
                                                                                                   ccACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTg                             <  4:121730/135‑1 (MQ=255)
                                                                                                    cACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGACTGGCCCTGGATCAGACAGGTTGAACCTTGACAGCCTGCATGCCTTTCTGGCCTTGGACTTCTTCGAAGCTGACTTGCTgg                            >  1:150002/1‑135 (MQ=255)
                                                                                                    cACTATTCTACGGGCAAAAAAAAGCCCCTGCACTAGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTgg                            >  4:237133/1‑135 (MQ=255)
                                                                                                            tACGGGCAAAAAAAAGCCCCTGCAGTTGGCAGTGGCTTTTCATAGTCTTGCGCTGGATCAGACAGGTTGTACCTTGTCAGCCTGCATGCATTTCTGGCCTTGGACTGCTTAGAAGGTGACTTGCTGGCcttctt                     <  4:259515/134‑1 (MQ=255)
                                                                                                             aCGGGAAAAAAAAAGCCCCTGCACTAGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTTTc                   <  2:158730/135‑1 (MQ=255)
                                                                                                               gggCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGCCCTTCTTTc                   <  4:359207/133‑1 (MQ=255)
                                                                                                               gggCAAAAAAAAGCCCCTGCACTAGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCttt                    <  3:237133/132‑1 (MQ=255)
                                                                                                                    aaaaaaaGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTTCTGGCCTTTTTTCAGGctct            >  6:29668/1‑135 (MQ=255)
                                                                                                                    aaaaaaaGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTTTCAGGctct            >  5:5441/1‑135 (MQ=255)
                                                                                                                    aaaaaaaGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTTTCAGGctct            >  8:169714/1‑135 (MQ=255)
                                                                                                                      aaaaaGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTTTCAGGCTCtt           >  6:168644/1‑134 (MQ=255)
                                                                                                                      aaaaaGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTTTCAGGCTCtt           >  7:288334/1‑134 (MQ=255)
                                                                                                                      aaaaaGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTGTCAGGCTCtt           >  6:44380/1‑134 (MQ=255)
                                                                                                                      aaaaaGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTGCGAAGGTGACTTGCTGGCCTTCTTTCCGGCTCtt           >  6:49524/1‑134 (MQ=255)
                                                                                                                      aaaaaGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACACGTTGAACCTTCTCAGCCGGCATGCCTTTCTGGCCTTGCACTGCATCGAAGGTGACTTGCTCGCCTTCTTTCAGGCTCtt           >  4:118796/1‑134 (MQ=255)
                                                                                                                      aaaaaGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCGGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGACTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTTTCCGGCTCtt           >  8:9361/1‑134 (MQ=255)
                                                                                                                              ccTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTTTCAGGCTCTTGAAGCCGtt  >  7:72368/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                              
AGAACATCGCTGCCAGGCGCCGGCCCAGGCCGACCGGTGGAAAATCCCCCTGGGGAGTGAGTAGAGGCTTGGACATGCAGGACGCTCCAAAAGACGAGCCCACTATTCTACGGGCAAAAAAAAGCCCCTGCACTTGGCAGGGGCTTTTCAAAGTCTGGCGCTGGATCAGACAGGTTGAACCTTGTCAGCCTGCATGCCTTTCTGGCCTTGGACTGCTTCGAAGGTGACTTGCTGGCCTTCTTTCAGGCTCTTGAAGCCGTT  >  KT2440/1116341‑1116601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑